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Nanoparticle-mediated microwave treatment methods

A nanoparticle, microwave technology, applied in microwave therapy, microwave surgical instruments, X-ray/γ-ray/particle irradiation therapy, etc., can solve the problem of no investigation, no research on therapeutic applications, etc.

Inactive Publication Date: 2011-05-11
CORNELL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However these aforementioned studies did not investigate whether enhanced heating from nanoparticles could be achieved in vivo, i.e. whether microwave irradiation generates more heat in tissue targeted with nanoparticles than in tissue alone
They also did not investigate whether the thermal differential achieved by microwaves was sufficient for therapeutic applications while maintaining safe temperatures in non-targeted tissues

Method used

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  • Nanoparticle-mediated microwave treatment methods

Examples

Experimental program
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Effect test

example 2

[0237] 6.2 Example 2: Functionalization of Nanoparticles to Make Functional Nanoparticles

[0238] The carboxy-terminated functionalized nanoparticles obtained from phospholipid functionalization described above can be further modified by covalent attachment of J591 antibody. In both cases, EDC (1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride), and thio-NHS (N- Hydroxysulfosuccinimide) (Pierce Biotechnology, Rockford, IL USA) converted the carboxyl groups on the nanoparticles into primary amine-reactive NHS-esters.

[0239] J591 was then added to the NHS-ester-modified particles suspended in phosphate-buffered saline at 10-fold concentration relative to the nanoparticle concentration. The mixture is allowed to react for 2 hours, during which time the NHS-esters will react with primary amines on the protein to form stable amide bonds. Excess unbound protein was then coupled to nanoparticles by size exclusion chromatography using an FPLC system (Superdex 200 size e...

example 3

[0241] 6.3 Example 3: In Vitro Cell Culture Studies

[0242] In vitro studies on prostate epithelial cells can be performed in order to assess the targeting capabilities of the nanoparticle conjugates as well as nanoparticle directed polymer formation. For these experiments, functionalized nanoparticles were stained with the hydrophobic fluorescent dye acridine orange, which was loaded into hydrophobic regions of phospholipids that encapsulated the nanoparticles and allowed visualization of the nanoparticles by fluorescent confocal microscopy. Aggregates.

[0243] According to the manufacturer's protocol, in their respective media at 75 cm 2 Immortal benign prostatic hyperplasia endothelial cells expressing PSMA (BPH-1, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany) and prostate endothelial carcinoma cells not expressing PSMA (PC-3, American Type Culture Collection) were cultured in 'T-flasks'. , Rockville, MD, USA). These cells were then subc...

example 4

[0249] 6.4 Example 4: Synthesis of Nanoparticles

[0250] 6.4.1 Growth of nanoparticles

[0251] This example demonstrates the successful synthesis and functionalization of magnetic nanoparticles (MNPs).

[0252] Nanoparticles were synthesized according to the method of Jun et al. (2005, Nanoscale Size Effect of Magnetic Nanocrystals and Their Utilization for Cancer Diagnosis via Magnetic Resonance Imaging. JACS, 2005.127: p.5732-5733). In short, the 4-nm Fe 3 o 4 Nanoparticles and Fe(acac) 3 Mix under nitrogen in phenyl ether, 1,2-hexadecanediol, oleic acid, and oleylamine, then heat to 260° C. and reflux for 30 minutes. After cooling to room temperature, black magnetite crystals were isolated by addition of excess ethanol followed by centrifugation. This produces monodisperse MNPs with a coating of oleic acid ( Figure 1A -C).

[0253] 6.4.2 Nanoparticle coatings

[0254] The platform functionalization strategy used in this work followed the method of Benoit Dubertret...

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Abstract

A method is provided for using magnetic nanoparticles to enhance microwave therapies for treating cells and tissues. The nanoparticles are designed to transduce microwave radiation into heat and furthermore, the nanoparticles may include specific tissue targeting and other functionality for enhancing in situ effects. In one embodiment, nanoparticles are introduced into a tissue system and a microwave field is applied. The nanoparticles react to the microwave energy by releasing heat thus heating the tissue and inducing hyperthermia (below 50 DEG C) or thermotherapy (above 50 DEG C). The nanoparticles can be designed for optimal heat production response at specific microwave frequencies and / or ranges of microwave frequencies where these frequencies may span the entire microwave spectrum, namely 300 MHz (3108 Hz) to 300 GHz (31011 Hz).

Description

[0001] Cross References to Related Applications [0002] This application claims priority and Interest, this application is hereby incorporated by reference in its entirety. 1. Technical field [0003] The present invention relates to magnetic nanoparticles and nanoparticle-mediated microwave treatment methods. The present invention also relates to methods for treating tumors and cancers using nanoparticle-mediated microwave thermotherapy. The invention further relates to systems for administering nanoparticle-mediated microwave therapy. 2. Technical background [0004] 2.1 Benign prostatic hyperplasia (BPH) [0005] The prostate of a healthy human male is slightly larger than a walnut and generally increases in size with age. More than half of men in the United States between the ages of 60 and 70 and as many as 90 percent of men between the ages of 70 and 90 have symptoms of benign prostatic hyperplasia (BPH), also known as benign prostatic hypertrophy or enlarged pros...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B18/18A61N5/02
CPCA61B18/18A61B18/1815A61B2017/00274A61B2018/00547A61K9/0009A61K9/5094A61N1/406A61N5/02A61N2005/1098A61P35/00
Inventor A·E·特C·A·拜特D·A·雷伊
Owner CORNELL UNIVERSITY
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