Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation and application of low-toxicity effective fraction for suppressing angiogenesis in cowherb seed

A technology of effective parts and angiogenesis, applied in the field of low-toxic effective parts, can solve the problems of large dosage and long treatment time, and achieve the effect of small side effects and high curative effect

Inactive Publication Date: 2011-05-25
JIANGNAN UNIV
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the currently marketed drugs that inhibit tumor angiogenesis can only be administered intravenously, and the dosage is large and the treatment time is long.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation and application of low-toxicity effective fraction for suppressing angiogenesis in cowherb seed
  • Preparation and application of low-toxicity effective fraction for suppressing angiogenesis in cowherb seed
  • Preparation and application of low-toxicity effective fraction for suppressing angiogenesis in cowherb seed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Extraction and Separation Process of Effective Fractions of Wang Buliuxing and Identification of Physicochemical Properties

[0030] 50 g of blancia, 10 times the amount of 75% ethanol was heated and extracted twice under continuous reflux, the extracts were combined, the solvent was recovered under reduced pressure, and the total extract was obtained after freeze-drying. Take 500 mg of the ethanol reflux extract and add water to suspend it, then extract with petroleum ether, dichloromethane, ethyl acetate, and water-saturated n-butanol. The obtained n-butanol component was rotary evaporated to an extract, redissolved in water, and freeze-dried, with a yield of 1.26% of the original medicinal material. The Molish reaction of the effective part is purple, the Libermann-Buchard reaction is purple, and the color of antimony trichloride is red. This result shows that this effective part contains at least one or more glycoside compounds.

Embodiment 2

[0031] Example 2: Inhibition of HMEC Cell Proliferation by Effective Fractions of Buliuxing

[0032] HMECs were incubated with MCDB-131 medium containing 1 mmol / L glutamine, 1 mg / L hydrocortisone, 10 μg / L EGF and 150 mL / L calf serum at 37°C in 50 mL / L CO 2 Subculture in an incubator. Take HMEC cells in the logarithmic growth phase, inoculate 8000 cells per well in a 96-well plate, and place in 37°C, 50mL / L CO 2 Incubate in an incubator for 24 hours, add different concentrations of drugs and act for 48 hours, take the group without drug addition as the negative control group, set 3 replicate wells for each group, and repeat the test 3 times for each batch of samples. Incubate for 72 hours after adding the drug, remove the supernatant, gently add 100 μL of 100 g / L trichloroacetic acid to each well for fixation, let it stand for 5 minutes, move to 4 ° C and place it for 40 minutes, pour off the fixation solution, and wash 5 times with deionized water , air dry. Add 100μL of 4g...

Embodiment 3

[0034] Example 3: Inhibitory effect of effective fractions of bulicula xyloides on HMEC migration in vitro

[0035] Using streak burn method to study cell migration [6. Qiao Hongwang, Kang Pengcheng, Tian Mohan et al. Effect of angiostatin on gastric cancer cells [J]. Advances in Anatomical Science, 2010, 16(34): 315-318], conventionally cultured Digest suspension of HMEC cells at 10 x 10 per well 4 cells / mL were added to a 24-well plate, 1 mL per well, and placed at 37°C, 5% CO 2 Incubate in an incubator with saturated humidity for 24 hours. After the cells adhere to the wall, use a pipette plastic tip to draw a burn zone about 1 mm wide in the center of each well, then wash off the exfoliated cells with PBS, and add 10 μg / mL drug concentration For cell culture medium, an equal amount of complete medium was added to the control group. Then take a picture under a 100× microscope. At this moment, it is a picture of cell migration at 0h. It was taken at the same field of view ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method and application of a low-toxicity effective fraction for suppressing angiogenesis in cowherb seed. The preparation method comprises the following steps of: heating and extracting cowherb seed with 75 percent of ethanol; and extracting the extract with petroleum ether, dichloromethane, ethyl acetate and water-saturated normal butanol in turn so as to obtain the effective fraction for suppressing angiogenesis in cowherb seed. Angiogenesis is taken as a target spot for treating a disease, and the medicinal curative effect and mechanism of the effective fraction in cowherb seed are searched and explained, so that scientific basis and important information are provided for the development of new application and finding of a new target spot with a medicinal effect. The effective fraction can be mixed with a pharmaceutically-applicable carrier so as to prepare various preparations. The effective fraction can be used for treating or preventing diseases related to angiogenesis and can be applied to treatment such as tumor chemotherapy and / or adjuvant chemotherapy.

Description

technical field [0001] The preparation process and application of the effective part of inhibiting angiogenesis in Buliuxing. The invention relates to a low-toxic and effective part extracted and separated from the traditional Chinese medicine Buliu Xing, which has the activity of inhibiting angiogenesis. Its preparation method and its new application in medicine, food and cosmetics are clarified. Background technique [0002] When a malignant tumor is larger than 1 mm in diameter and tends to metastasize, new tumor blood vessels must be formed first. Inhibition of neovascularization in and around tumors can effectively reduce or even eliminate tumors. In recent years, tumor angiogenesis has become an attractive hotspot in tumor therapy. At present, many angiogenesis inhibitors have been developed and are being studied in Phase I-III clinical trials, such as angiostatin (Angiostatin), endostatin (Endostatin), fumagillin analogues, metalloproteinase inhibitors, urokinase, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/36A61K8/97A61P9/00A61P35/00A61P27/06A61P9/10A61P27/02A61P43/00A61P17/06A61P19/02A61P29/00A61P9/14A61P17/00A61P17/02A61P17/08A61P17/10A61Q19/00A23L1/29A61K131/00A23L33/00
Inventor 金坚邱丽颖冯磊高越颖杜芳芳
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com