Nanomagnetic beads of coupled fluorescein and preparation method thereof

A technology of nano-magnetic beads and fluorescein, applied in the field of chemical synthesis, can solve the problems of unseen magnetic nano-products and inability to compete with nano-magnetic bead products.

Inactive Publication Date: 2013-06-12
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few related patented products in China, and it is still unable to compete with foreign imported nano magnetic bead products
Nanomagnetic beads coupled with fluorescein are a new compound, and there is no domestic patent disclosure. There are already imported series of fluorescently labeled nanoprobes in the domestic market, such as the Nanoprobe nano fluorescent probe series products, which use a new generation of quantum dot fluorescent labels technology, but no fluorescently labeled magnetic nano-products

Method used

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  • Nanomagnetic beads of coupled fluorescein and preparation method thereof
  • Nanomagnetic beads of coupled fluorescein and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) Divide the concentration of 100ml into 0.3mol L -1 of aniline with 0.5 mol L -1 Mix the hydrochloric acid solution, add 2g of γ-iron oxide into the mixed solution, and keep stirring. During this process, slowly add ammonium persulfate with a molar ratio of 1:1 to aniline, continue to stir for 6 hours, filter with two Wash with water and ethanol until the filtrate is colorless, and then use a concentration higher than 0.1mol L -1 The ammonia water was dedoped for not less than 2 hours to obtain intrinsic polyaniline. The product was suction filtered, washed with secondary water until the filtrate was colorless, and dried in vacuum to obtain γ-iron oxide / polyaniline. The pure nano-magnetic beads prepared in this step do not have fluorescent properties ( figure 1 B), its poor dispersion, easy to agglomerate, because of its superparamagnetic ( figure 2 ).

[0014] (2) Add γ-iron oxide / polyaniline and sodium fluorescein in step (1) to 0.05mol L at a mass ratio of 1:...

Embodiment 2

[0017] (1) Divide 100ml concentration into 0.1mol L -1 of aniline with 0.5 mol L -1 Mix the hydrochloric acid solution, add 1g of γ-iron oxide into the mixed solution, and keep stirring. During this process, slowly add ammonium persulfate with a molar ratio of 1:1 to aniline, continue stirring for 2-40 hours, and filter with suction. Wash with secondary water and ethanol until the filtrate is colorless, and then use a concentration higher than 0.1mol L -1 The ammonia water was dedoped for not less than 2 hours to obtain intrinsic polyaniline, the product was suction filtered, washed with secondary water until the filtrate was colorless, and dried in vacuum to obtain γ-iron oxide / polyaniline;

[0018] (2) Add γ-iron oxide / polyaniline and sodium fluorescein at a mass ratio of 1:0.5 to 0.05mol L -1 Protonic acid solution (such as hydrochloric acid solution), stirred for 10-48 hours, suction filtered, washed with water until the filtrate was colorless, and vacuum-dried at 80°C t...

Embodiment 3

[0020] (1) Divide 100ml concentration into 1.5mol L -1 of aniline with 0.5 mol L -1 Mix the hydrochloric acid solution, add 5g of γ-iron oxide into the mixed solution, and keep stirring. During this process, slowly add ammonium persulfate with a molar ratio of 1:1 to aniline, continue stirring for 2-40 hours, and filter with suction. Wash with secondary water and ethanol until the filtrate is colorless, and then use a concentration higher than 0.1mol L -1 The ammonia water was dedoped for not less than 2 hours to obtain intrinsic polyaniline, the product was suction filtered, washed with secondary water until the filtrate was colorless, and dried in vacuum to obtain γ-iron oxide / polyaniline;

[0021] (2) Add γ-iron oxide / polyaniline and sodium fluorescein at a mass ratio of 1:3 to 2mol L -1 in the protonic acid solution, stirred for 10-48 hours, suction filtered, washed with water until the filtrate was colorless, and vacuum-dried at 80° C. to obtain nano magnetic beads coup...

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Abstract

The invention relates to nanomagnetic beads of a coupled fluorescein and a preparation method thereof. The method comprises the following specific steps: mixing aniline with a hydrochloric acid solution, adding gamma-iron oxide in the mixed solution, stirring continuously, slowly adding aniline in ammonium persulfate, stirring again, performing suction filtering, washing filtrate with secondary water and ethanol until the filtrate is colorless, then doping with ammonia water to obtain polyaniline in an eigen state, performing suction filtering on the product, washing filtrate with secondary water until the filtrate is colorless, and performing vacuum drying to obtain gamma-iron oxide / polyaniline; and adding gamma-iron oxide / polyaniline and fluorescein sodium in a protonic acid solution, stirring, performing suction filtering, washing filtrate with water until the filtrate is colorless, and performing vacuum drying to obtain the nanomagnetic beads of the coupled fluorescein. By using the nanomagnetic beads for fluorescent tracing provided by the invention, the distribution of the nanomagnetic beads under a complicated internal environment in an animal experiment of the nanomagneticbeads can be detected; and in the further medical application stage, the nanomagnetic beads can be used for the tracing of a nanomagnetic bead medicament, thus the site of action and concentration ofthe medicine can be determined.

Description

technical field [0001] The invention mainly belongs to the technical field of chemical synthesis, and in particular relates to a nanometer magnetic bead coupled with fluorescein and a preparation method thereof. Background technique [0002] Nanomagnetic beads are a new type of biomedical material, which currently has good research uses, and its medical potential is even more attractive. Researchers in many fields are stepping up research and development. Almost all the series of reagent nano-magnetic beads in the domestic market come from foreign pharmaceutical companies, and a large amount of domestic research funds are spent on such expensive reagents every year. At present, there are few related patented products in China, and they cannot compete with imported nano-magnetic bead products. Nanomagnetic beads coupled with fluorescein are a new compound, and there is no domestic patent disclosure. There are already imported series of fluorescently labeled nanoprobes in the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G73/02
Inventor 冀德君郭晓霞阚锦晴
Owner YANGZHOU UNIV
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