Composite fungicide for rapid degradation of organic waste and applications thereof

A technology of organic waste and compound bacterial agent, which is applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of unbalanced culture medium nutrients, unbalanced growth of strains, easy contamination of miscellaneous bacteria, etc., and shorten the maturity time , enhance the activity, and accelerate the effect of the combined composting process

Active Publication Date: 2011-06-15

王杰

2 Cites 18 Cited by

AI-Extracted Technical Summary

Problems solved by technology

At present, the production process of this kind of bacterial agent products on the market is simple and extensive, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Abstract

The invention discloses a composite fungicide for rapid degradation of organic waste and applications thereof. The composite fungicide is a liquid fungicide prepared by mixing a functional fungicide and a characteristic fungicide in a volume ratio of (2-4):1, and is used in the technical field of treatment of municipal solid waste and livestock and poultry manure and sludge reduction. The composite fungicide disclosed by the invention has the advantage that the functional fungicideand the characteristic fungicide are obtained by pure culture, so that the function of the fungicide is enhanced and simultaneously the stability of the fungicide is improved. When the product is used in waste treatment in a composting plant, a refuse treatment plant or a personal treatment yard, the activity of the total microflora and lignocellulose-degrading microorganisms in a composting pile can be enhanced, the combined composting process of vegetables, flowers and straws can be distinctly accelerated, and the decomposing time is shortened.

Application Domain

BacteriaMicroorganism based processes +1

Technology Topic

FungicideLivestock +9

Image

Examples

Experimental program(4)

Example Embodiment

[0013] Example 1 1. Materials and source strains: Perchlorate degrading bacteria (Azospira oryzae; DSMZ13638) and Caldilinea aerophila (DSMZ14535) were purchased from the German Collection of Microbial Cultures (DSMZ).

[0014] Methylocella silvestris (Methylocella silvestris; ATCC700799), Nitrobacter winogradskyi (ATCC25391), Nitrosomonas europaea (ATCC25391) were purchased from the American Microbial Species Collection (ATCC).

[0015] Bacillus subtilis (ACCC10619), Brevibacillus brevis (ACCC10248), Cellulomonas cartae (ACCC10527), Pseudomonas sp. (ACCC10677) were purchased from Chinese agricultural microorganisms Kind of resource library (ACCC).

[0016] Acetobacterium wieringae (Acetobacterium wieringae; CGMCC 1.2033) was purchased from China Common Microbial Species Resource Bank (CGMCC). Rhodanobacter fulvus (CICIM B1950) was purchased from Jiangnan University Microbial Species Resource Bank (CICIM) 2. Cultivation Base and preparation slant seed preparation: Streak and separate the strain from the original strain under aseptic conditions, put it in an incubator at 25-35℃, and select a single colony to connect to the slant medium to continue cultivation.

[0017] Bacillus subtilis and Bacillus brevis use LB medium: tryptone 10g/l, yeast extract 5g/L, NaCl10g/l. Nitrate culture medium: beef extract 3g/L, peptone 5g/L, KNO 3 1g/L, Nitrosomonas using nitrite medium: KNO 3 2g/L, Mg 2 SO 4 ? ? 7H 2 O 0.2g/L, K 2 HPO 0.5g/L, potassium sodium tartrate 20g/L.

[0018] Cellulomonas faecalis culture medium: maltose 10g/L, peptone 5g/L, yeast powder 2g/L, K 2 HPO 4 2g/L, Mg 2 SO 4 ? ? 7H 2 O 0.5g/L.

[0019] Acetobacter wiskeri culture medium: KCl 0.33g/L, MgCl 2 ? ? 6H 2 O 0.52g/L, CaCl 2 ? ? 2H 2 O 0.22g/L, NH 4 Cl 0.33g/L, KH 2 PO 4 0.33g/L, yeast extract 0.5g/L, NaHCO 3 1g/L, fructose 10g/L, Na 2 S? ? 9H 2 O 0.7g/L.

[0020] Medium for perchlorate degrading bacteria and Microbacterium Luohe: yeast extract 0.5g/L, peptone 0.5g/L, casein 0.5g/L, glucose 0.5g/L, soluble starch 0.5g/L, K 2 HPO 4 0.3g/L, MgSO 4 ·7H 2 O 0.05g/L.

[0021] Pseudomonas and Bacillus brevis culture medium: peptone 5.0g/L, beef extract 3g/L, MnSO4·H2O 0.01g/L, pH 7.0.

[0022] Warm rope fungus culture medium (g/l): KH 2 PO 4 0.14g/L, MgCl 2 ·6H 2 O 0.2g/L, CaCl 2 ·2H 2 O 0.15g/L, NH 4 Cl 0.54g/L, yeast extract 2.3g/L, glucose 2.2g/L, NaHCO 3 2.5g/L, pH7.0.

[0023] Methanoxidizing bacteria culture medium: KNO 3 0.25g/l, KH 2 PO4 0.1g/L, MgSO 4 ·7H 2 O 0.05g/L, CaCl 2 ·2H 2 O 0.01g/L, yeast extract 1g/L, methanol 5g/L.

[0024] Both the shake flask and the expansion culture medium use PDA medium: peel the potatoes and cut into pieces; weigh 200g, add water and boil for 30min (pay attention to the control of firepower, and add water appropriately), filter with eight layers of gauze, and add 20g glucose to the filtrate. Make up to 1000ml of water.

[0025] Three, compound bacteria and preparation such as figure 1 As shown, a preparation method of a composite bacterial agent for rapidly degrading organic waste is as follows: The composite bacterial agent includes two parts: a functional bacterial agent and a characteristic bacterial agent. The functional bacterial agent and the characteristic bacterial agent are compatible in a volume ratio of 3:1.

[0026] Compound bacteria expansion method: After PDA medium is used as the liquid fermentation medium, the inoculum is 1-5%, 15-38℃, after 1-2 days of anaerobic fermentation in a closed container, aeration is carried out with an aeration pump. Above 0.15vvm, culture for 5-10 days.

[0027] Mixing the liquid bacterial agent prepared by compound mixing with activated sludge can be made into a mud-like bacterial agent; mixing the liquid bacterial agent prepared by compound mixing with sawdust can make a powdery bacterial agent.

[0028] Bacillus subtilis (Bacillus subtilis), Nitrobacter winogradskyi (Cellulomonas cartae), Pseudomonas (Pseudomonas sp.) cultivation method: maintain the temperature at 15-38 ℃, ventilation rate 0.15vvm , Cultivate for 2-4 days.

[0029] The culturing method of Nitrosomonas europaea: maintain the temperature at 15-38°C and anaerobic fermentation in a closed container for 3-5 days.

[0030] Functional inoculum mixed and expanded culture: The above-mentioned functional inoculum is connected to the PDA medium at 5% of the inoculum, and the temperature is maintained at 15-38°C with a ventilation rate of 0.15 vvm for 3-5 days.

[0031] The characteristic microbial agents in the microbial complex are composed of Acetobacterium wieringae, Azospira oryzae, Brevibacillus brevis, Caldilinea aerophila, and Methylocella silvestris) and Rhodanobacter fulvus (Rhodanobacter fulvus) any 3 or more than 3 kinds of bacteria.

[0032] For example: the seed liquid contains three kinds of bacteria: Acetobacterium wieringae, Azospira oryzae and Brevibacillus brevis; or they contain perchlorate degradation Bacteria (Azospira oryzae), Brevibacillus brevis, Caldilinea aerophila, Methylocella silvestris 4 species; or contain Acetobacterium wieringae, perchlorate Degrading bacteria (Azospira oryzae), Brevibacillus brevis, Caldilinea aerophila and Methylocella silvestris 5 strains; or containing Acetobacterium wieringae, perchloric acid Salt-degrading bacteria (Azospira oryzae), Brevibacillus brevis, Caldilinea aerophila, Methylocella silvestris and Rhodanobacter fulvus 6 species, etc.

[0033] Characteristic bacterial preparation method, (refer to the preparation flow chart, the mixed seed liquid can be compounded by equal amount of pure culture seed liquid of each strain, or the mixed mixed seed liquid can be used as the seed to be connected to the fermentation medium for expansion culture ) Prepare the seed culture medium of each strain according to the conventional method. According to the requirements, part (≥3) or all of the above-mentioned 6 kinds of characteristic bacteria are connected to the above-mentioned characteristic culture medium for mixed culture, and the temperature is maintained at 15 -38℃, incubate in a closed container for 3-5 days. The extended culture of the characteristic bacteria compound solution can also connect 5-10% of the characteristic bacteria compound solution to the optimized PDA medium for compound cultivation, keeping the temperature at 15-38℃, and culturing in a closed container for 3-5 days.

Example Embodiment

[0034] Example 2 Application of the compound bacterial agent in the degradation rate of sludge. The bacterial agent of the above example 1 was applied to the sludge reduction of the Ministry of Environment and Forestry of Gifu Prefecture, Japan. The operation method was to add 20g of the sludge containing the compound bacterial agent to the test tube. The group added 4g of normal sludge, added sludge for 36 consecutive days, and removed the supernatant liquid. The following data is obtained. After the addition of bacterial agents, the sludge degradation rate is significantly accelerated, and the added sludge is almost completely degraded, and the maximum sludge reduction load is 0.041d -1 , Only 0.018d without adding bacteria -1.

[0035]

Example Embodiment

[0036] Example 3 Application of composite bacterial agents in the treatment of sewage from pig farms and rural villages. Liquid active bacterial agents are mixed with activated sludge to prepare sludge bacterial agents. The activated sludge method is used to treat sewage from pig farms and rural villages. Table 2 and Table 3 show that the mud-like active bacteria agent has a good treatment effect on the sewage of pig farms and rural villages. Among them, the organic components are almost completely degraded, the BOD removal rate is more than 99%, and the removal of nitrogen and phosphorus is also good. Effect.

[0037] Item

[0038] Item

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to view more

PUM

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to view more

Classification and recommendation of technical efficacy words

High activity

Composite fungicide for rapid degradation of organic waste and applications thereof

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Image

Examples

Example Embodiment

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

Similar technology patents

Lensing spherical vaterite calcium carbonate crystal with high purity and preparation method thereof

Subtilases

Method for preparing high-performance solid desulfurizing agent by carbide slag slurry

Cytoplasmic Localization Dna and Rna

Catalyst composition preparation and use

Classification and recommendation of technical efficacy words

Automated cooking control via enhanced cooking equipment

Commerce-enabled environment for interacting with simulated phenomena

Ethylidene acenaphthene (alpha-diimine) nickel olefin catalyst, and preparation method and application thereof

Method for preparing high-concentration biological deodorant

Fluorinated and phosphor-contained hydrogenation catalyst with silicon oxide-alumina as carrier and its production