Preparation method of novel injectable polypeptide hydrogel

A hydrogel, a new type of technology, applied in the field of preparation of injectable polypeptide hydrogel, can solve the problems of complex bone defect shape, high price, restrictions on popularization and application, etc., and achieve the effect of good bone tissue compatibility

Inactive Publication Date: 2011-06-22
THE STOMATOLOGIAL HOSPITAL OF ZHEJIANG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the shape of bone defects is often very complex, especially in the oral and maxillofacial region, such as alveolar bone defects caused by periodontitis, bone resorption caused by cysts and tumors, and irregular gaps and congenital bone defects during jaw restoration. The ideal scaffold model cannot be prefabricated due to gaps, etc., which

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] First, high-purity polypeptides with osteogenic activity were synthesized (in powder form, the amino acid sequences of which are RADARDARADARADA, RADARGDARADARGDA, R is arginine, A is alanine, D is aspartic acid, and G is glycine). Prepare the polypeptide material with deionized water to make a solution of 1% concentration (such as 10mg of polypeptide dissolved in 1ml of deionized water), then mix the two solutions at a ratio of 1:1 (volume ratio), and finally add a small amount of The alpha-MEM medium solution, under the conditions of 37°C and pH 7, after standing still for several minutes, the gel gradually formed. In this way, a biomimetic self-assembled polypeptide hydrogel material with osteogenic activity is obtained, which is also an excellent biomimetic scaffold material for three-dimensional culture of osteoblasts.

Embodiment 2

[0016] First, high-purity polypeptides with osteogenic activity were synthesized (in powder form, the amino acid sequences of which are RADARDARADARADA, RADARGDARADARGDA, R is arginine, A is alanine, D is aspartic acid, and G is glycine). Prepare the polypeptide material with deionized water to make a solution with a concentration of 1% (such as 10mg of polypeptide dissolved in 1ml of deionized water), then mix the two solutions at a ratio of 1:1 (volume ratio), and use a sterile syringe The mixed solution is injected into the irregularly shaped bone defect area, and under the induction of tissue fluid (such as blood), self-assembles in situ to form a hydrogel, which is used as a scaffold material to promote the repair of bone defects.

Embodiment 3

[0018] First, high-purity polypeptides (in powder form, whose amino acid sequences are RADA16, R is arginine, A is alanine, and D is aspartic acid) are synthesized first. The polypeptide material is made into a solution with a concentration of 1% with deionized water, and then a certain amount of rhOP-1 is added to form an OP-1 polypeptide hydrogel slow-release system (such as 10 mg of polypeptide is dissolved in 1 ml of deionized water, and then added 2ug rhOP-1), under the conditions of 37°C and pH 7, hydrogel is formed after standing for several minutes, and the polypeptide hydrogel system has the function of OP-1 sustained release.

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Abstract

The invention discloses a preparation method of a novel injectable polypeptide hydrogel. A polypeptide hydrogel bionic scaffold with osteogenic activity is formed by a polypeptide solution with a specific sequence under a condition with a temperature of 37 DEG C and a pH of 7 based on the self-assembly principle. The method has a simple preparation process; the hydrogel has simple and controllable components and high safety, is injectable before complete self-assembly, can perform in-situ self assembly at gaps with any form between bone defects; the hydrogel can be used as a bionic scaffold material to promote bone repair, and after bone growth factors are added, the hydrogel can be used as a carrier to control the release of the growth factors so as to shorten the treatment course. A culture medium can be added into the system as required, and the system can be used as a bionic scaffold for the three-dimensional culture of osteoblasts.

Description

technical field [0001] The invention relates to a preparation method of a novel injectable polypeptide hydrogel. In particular, it relates to a polypeptide hydrogel scaffold with osteogenic activity formed by the principle of self-assembly. The system is injectable and can fill the gap of bone defect of any shape to form a biomimetic active scaffold that promotes bone regeneration. Background technique [0002] Tissue engineering is a new interdisciplinary subject proposed and established in the middle and late 1980s. The establishment and development of bone tissue engineering has opened up a new research field for exploring and seeking ideal bone regeneration. Since Bagambisa discovered in 1994 that osteoblasts are selective to materials, people have been working hard to find a scaffold material that is more suitable for cell growth. At present, bone tissue engineering scaffold materials can be divided into bioactive ceramics, bio-derived bone scaffold materials, and deg...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/52A61L27/58A61L27/54C12N5/077
Inventor 谢志坚张锋胡飞琴张小磊石珏吴铭芳
Owner THE STOMATOLOGIAL HOSPITAL OF ZHEJIANG UNIV SCHOOL OF MEDICINE
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