Preparation method of novel injectable polypeptide hydrogel

Abstract

The invention discloses a preparation method of a novel injectable polypeptide hydrogel. A polypeptide hydrogel bionic scaffold with osteogenic activity is formed by a polypeptide solution with a specific sequence under a condition with a temperature of 37 DEG C and a pH of 7 based on the self-assembly principle. The method has a simple preparation process; the hydrogel has simple and controllable components and high safety, is injectable before complete self-assembly, can perform in-situ self assembly at gaps with any form between bone defects; the hydrogel can be used as a bionic scaffold material to promote bone repair, and after bone growth factors are added, the hydrogel can be used as a carrier to control the release of the growth factors so as to shorten the treatment course. A culture medium can be added into the system as required, and the system can be used as a bionic scaffold for the three-dimensional culture of osteoblasts.

Description

technical field [0001] The invention relates to a preparation method of a novel injectable polypeptide hydrogel. In particular, it relates to a polypeptide hydrogel scaffold with osteogenic activity formed by the principle of self-assembly. The system is injectable and can fill the gap of bone defect of any shape to form a biomimetic active scaffold that promotes bone regeneration. Background technique [0002] Tissue engineering is a new interdisciplinary subject proposed and established in the middle and late 1980s. The establishment and development of bone tissue engineering has opened up a new research field for exploring and seeking ideal bone regeneration. Since Bagambisa discovered in 1994 that osteoblasts are selective to materials, people have been working hard to find a scaffold material that is more suitable for cell growth. At present, bone tissue engineering scaffold materials can be divided into bioactive ceramics, bio-derived bone scaffold materials, and deg...

AI Technical Summary

Problems solved by technology

However, the shape of bone defects is often very complex, especially in the oral and maxillofacial region, such as alveolar bone defects caused by periodontitis, bone resorption caused by cysts and tumors, and irregular gaps and congenital bone defects during jaw restoration. The ideal scaffold model cannot be prefabricated due to gaps, etc., which seriously restricts the application prospect of bone tissue engineering in stomatology

[0003] At present, the bone filling materials used in clinical oral cavity in China are all monopolized by foreign brands, such as granular bio-oss, etc., but the price is expensive and the operation is complicated, which seriously restricts its clinical application.