Method for preparing catechin monomers

A technology of catechins and monomers, which is applied in the direction of anti-toxic agents, metabolic diseases, anti-viral agents, etc., can solve the problems of difficult large-scale promotion, high cost, and large pollution, and achieve environmental friendliness, low cost, and high safety Effect

Inactive Publication Date: 2011-07-06
温尧林
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] The technical problem to be solved by the present invention is to provide a method for preparing monomeric catechins. The method described in the prior art needs to solve the problems of high cost, heavy pollution and difficulty in extracting monomeric catechins. Technical Issues for Large-Scale Rollout

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  • Method for preparing catechin monomers
  • Method for preparing catechin monomers
  • Method for preparing catechin monomers

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1 The preparation of green tea extract

[0035] Add 80L of pure water to 10kg of green tea, heat to 70°C, stir (speed 20r / min) to extract for 40min, filter, the filtrate is cooled to 30°C by heat exchange, add 80L of pure water to the filter residue, stir and elute at room temperature for 30min, and combine the extracts; 10000r / min continuous centrifugation to obtain a clarified green tea extract, which is concentrated and stored in a storage tank. Part of the extract was weighed after drying, and the calculated extraction rate was 26.8%. According to high performance liquid chromatography analysis, the EGCG content was 8.29%; the ECG content was 1.83%.

Embodiment 2

[0036] Embodiment 2 Preparation of monomer EGCG and ECG compounds of the present invention

[0037] The green tea extract in Example 1 is adsorbed by macroporous resin; according to the amount of 1kg tea extract 1.5L resin (fully swollen in 25°C water) for adsorption, after sample loading, wash 10CV (column bed volume) Finally, three column volumes were eluted with 95% ethanol, and the enriched catechin mixture weighed 78 g, wherein the EGCG content was 28.5%, and the ECG content was 6.29%.

[0038] The above-mentioned catechin mixture is adsorbed by reversed-phase chromatography filler RP-C18, and eluted with 15%, 25%, 35%, and 95% concentration of ethanol aqueous solution for 3 times of column volume respectively, and the eluted fractions of 25% and 35% are collected respectively After concentration, the 25% part weighs 22g, and the EGCG content is 90%; after the concentration, the 35% part weighs 4.8g, and the ECG content is 92%; it is separated by gel, and 90% ethanol is u...

Embodiment 3

[0039] Embodiment 3 Preparation of monomer EGCG and ECG compounds of the present invention

[0040] The green tea extract in Example 1 is adsorbed by polyamide resin; it is adsorbed according to the amount of 2L resin (fully swollen in 25°C water) of 1kg of tea leaves. Elute with % ethanol for three column volumes and concentrate to obtain an enriched catechin mixture weighing 73 g, in which the EGCG content is 30.4%, and the ECG content is 6.7%.

[0041] The above-mentioned catechin mixture is adsorbed by reversed-phase chromatography filler RP-C18, and eluted with 15%, 25%, 35%, and 95% concentration of ethanol aqueous solution for 3 times of column volume respectively, and the eluted fractions of 25% and 35% are collected respectively After concentration, 25% part weighs 22g, and the EGCG content is 91%; after concentration, 35% part weighs 4.8g, and ECG content is 93%; Gel is used to separate, and 95% ethanol is used as mobile equal degree elution, according to the analysi...

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Abstract

The invention discloses a method for preparing catechin monomers, which comprises the following steps of: pretreating water extraction solution of tea or tea tailings; enriching a catechin mixture; adsorbing the catechin mixture by using a reversed-phase chromatograph packing; performing gradient elution; performing gel filtration chromalography on eluent; and concentrating, crystallizing, and drying. The invention also provides catechin monomers of epigallocatechin gallate (EGCG) and epicatechin gallate (ECG) prepared by the method. The method is simple and practicable, low in cost, stable in process and easy to promote. The EGCG and ECG monomers produced by the method can be used for preparing health foods and cosmetics, or used as standards.

Description

Technical field: [0001] The invention relates to an extraction process of traditional Chinese medicine, in particular to a preparation method of high-purity monomeric catechin. Background technique: [0002] Tea catechin is the main functional component of green tea, which has many health care and pharmacological effects such as fat-lowering weight loss, anti-aging, anti-oxidation, anti-bacteria, anti-virus, etc., and has attracted widespread attention in recent years. Tea catechins mainly include catechin (C), epicatechin (EC), gallocatechin (GC), epigallocatechin (EGC), catechin gall Catechin-3-O-gallate, CG, epicatechin-3-O-gallate, ECG, gallocatechin-3-O-gallate, GCG ), the structures of epigallocatechin-3-O-gallate (EGCG) are shown below, and the compounds EGCG and ECG are the two compounds with the highest content and the best activity, and are in great demand. [0003] [0004] [0005] R 1 R 2 [0006] C H H [0007] CG H Galoyl [0008] GC OH H [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62A61K31/353A61K8/49A61P3/04A61P3/06A61P39/06A61P31/04A61P31/12
Inventor 温尧林
Owner 温尧林
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