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Spice extractum prepared by waste tobacco leaf, preparation method thereof and application thereof

A technology for waste tobacco leaves and tobacco flavors, which is applied in the field of tobacco flavor extracts, can solve the problems of low reutilization of waste tobacco leaves, achieve large-scale industrial production, avoid waste of resources, and reduce usage

Active Publication Date: 2011-07-13
HONGYUN HONGHE TOBACCO (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In short, the current level of reuse of waste tobacco leaves is relatively low. How to effectively use these tobacco leaf resources and turn waste into treasure has become the key for tobacco companies to save costs and improve technological innovation capabilities.

Method used

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  • Spice extractum prepared by waste tobacco leaf, preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Medium and Culture Conditions

[0032] The strains Acinetobacter ND12 used in the experiment were inoculated in 50 mL of LB liquid medium, and cultured at 37 °C for 12 h to obtain liquid strains. The liquid strains were transferred to 1 L of LB liquid medium according to the inoculum amount of 5%, and cultured with shaking at 37°C and 150 r / min for 12 h. Centrifuge the culture solution at 3500 rpm / min for 10 min, wash the precipitate with sterile water, centrifuge, and finally shake it evenly with 20 mL of sterile water, and dilute it 10 times before use to obtain the bacterial agent.

[0033] Fermentation treatment of waste tobacco leaves

[0034] 1) Fermentation by nicotine-degrading bacteria

[0035] For every 200 g of low-grade tobacco leaves, 40 mL of nicotine-degrading bacteria (Acinetobacter ND12) inoculum was sprayed (the control sample was sprayed with an equal volume of sterile water). The treated tobacco leaf samples were fermented in a 22°C, 60% co...

Embodiment 2

[0056] 1. Medium and culture conditions: With embodiment 1.

[0057] Fermentation treatment of waste tobacco leaves

[0058] 1) Fermentation by nicotine-degrading bacteria

[0059] For every 200 g of low-grade tobacco leaves, 40 mL of nicotine-degrading bacteria (Acinetobacter ND12) inoculum was sprayed (the control sample was sprayed with an equal volume of sterile water). The treated tobacco leaf samples were fermented in a 22°C, 60% constant temperature and humidity box for 10 days for the next experiment.

[0060] 2) Mixed fermentation treatment of esterase-producing bacteria and aroma-producing bacteria

[0061] The samples fermented by nicotine-degrading bacteria were sprayed with 20 mL of ferulic esterase-producing bacteria (Bacillus pumilus Van35) and aroma-producing bacteria (Bacillus subtilis) (the control sample was sprayed with an equal volume of sterile water). The treated tobacco leaf samples were fermented for 20 days in a 22°C, 60% constant temperature ...

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Abstract

The invention discloses a spice extractum prepared by waste tobacco leaf, a preparation method thereof and application thereof. The preparation method comprises the steps of: taking the waste tobacco leaf as a raw material; sequentially fermenting acinetobacter, bacillus pumilus and bacillus subtilis to reduce nicotine in the waste tobacco leaf; transforming ferulic acid in the tobacco leaf to generate flavor ingredients such as the 4-guaethol, the vanillin and the like; and extracting the flavor ingredients in the waste tobacco leaf after fermenting to prepare the spice extractum. The spice extractum comprises higher-content flavor ingredients which are mainly to be benzyl alcohol, solanone, vanillin, BETA-damascenone, dihydro kiwifruit lactone, megastigmatrienone, duvatriene diol and neophytadiene. The result of the aromaticflvouring smoke panel test shows that the spice extractum has the effects of obviously dispatching the tobacco aroma, fining and smoothing the alcohol and the smoke, reducing the pungency, covering up the impure smell, and improving the absorbed smell of a cigarette.

Description

technical field [0001] The invention belongs to the technical field of tobacco additives, more specifically, the invention relates to a tobacco spice extract prepared by using waste tobacco leaves. At the same time, the invention also relates to a preparation method of the spice extract and its application in the tobacco industry. Background technique [0002] Tobacco is one of the important pillars of our country's finances. The State Tobacco Monopoly Administration clearly stated in Article 7 of the "Eleventh Five-Year" China Cigarette Science and Technology Development Outline that it is necessary to "overcome the key technologies that affect the aroma and quality of tobacco leaves" and narrow the quality gap with foreign tobacco leaves in order to improve domestic and foreign market competitiveness. . [0003] In the process of tobacco planting and processing, a large amount of tobacco waste such as low-grade tobacco leaves, tobacco stems and tobacco dust is produced, ...

Claims

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Application Information

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IPC IPC(8): A24B3/12A24B3/14C12N1/20C12R1/08
Inventor 段焰青张克勤者为叶灵夏建军杨金奎王文元
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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