Integrated dynamic inoculation culture method for osteogenous seed cell and matched bioreactor thereof

Abstract

The invention discloses an integrated dynamic inoculation culture method for an osteogenous seed cell and a matched perfusion type bioreactor thereof. A peristaltic pump, a perfusion chamber, a liquid storage bottle, a channel converter and a connection tube constitute a double loop structure for perfusion inoculation and perfusion culture. When the double loop structure is used, two loops are switched by regulating the channel converter, thereby achieving the effect of integrated dynamic inoculation and dynamic culture. The dynamic inoculation and dynamic culture of the cell are carried out through the perfusion type bioreactor, thereby enhancing the inoculation efficiency and uniformity of the cell, enlarging the range and efficiency of supplying nutrition and oxygen and simultaneously providing the shearing strength stimulus to the osteogenous cell so that the inoculation and culture of the cell are integrally carried out in the same environment, thus omitting a conversion link. Compared with the existing cell inoculation and culture technology, the invention has the advantage that a proper environment is provided for adhesion, distribution and differentiation of the cell and tissue construction, thereby obviously enhancing the effect of tissue engineering bone construction.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for increasing the effect of tissue construction in tissue engineering bone construction in vitro, in particular to an integrated method for dynamic inoculation and cultivation of seed cells for tissue engineering bone construction and a supporting perfusion biological reaction The device can significantly improve the material transmission efficiency and range in the scaffold carrier, increase the cell seeding efficiency and culture effect, and facilitate the in vitro construction of tissue engineered bone. Background technique [0002] At present, bone defect is a common problem in orthopedic clinic. However, during the course of treatment, due to soft tissue embedding, improper internal fixation, lack of blood supply, osteoporosis, metabolic diseases, infection and other reasons, delayed union or nonunion of fractures requires a second operation. Bone grafting is one of the...

AI Technical Summary

Problems solved by technology

Statically seeded cells usually rely on gravity, negative pressure, or capillary action to enter the scaffold, which is difficult to control, resulting in inefficient cell seeding and uneven distribution within the scaffold

The nutrient supply and gas exchange required for cell growth in static culture are realized through the concentration gradient difference of substances, that is, the diffusion effect, which makes the transmission efficiency and range of nutrients and oxygen to the inside of the scaffold very limited, resulting in insufficient nutrition and oxygen supply inside the scaffold. , causing cells to survive only around the scaffold, resulting in "hollowing" of the constructed tissue

In addition, cell inoculation and cell culture are generally two independent steps, requiring manual transfer of the scaffold material inoculated into the culture container, which obviously increases the risk of microbial contamination and also affects the effect of tissue construction

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