Method for storing endometrial stem cells

A technology of endometrial stem cells and cells, which is applied in the corresponding culture and storage technology field, and can solve problems such as ethical restrictions and difficulties in the source of stem cells
CN102154202AInactive Publication Date: 2011-08-17HANGZHOU S EVANS BIOSCI LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HANGZHOU S EVANS BIOSCI LTD
Publication Date
2011-08-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for storing endometrial stem cells, which comprises the following steps: 1) preparing a collection tool set and preparing a culture medium; 2) collecting menses; 3) performing a sterility test; and 4) subjecting endometrial stem cell to separation culture, namely, filtering mixed liquid, which is obtained by the step 2), in a collection tube 1, collecting single karyoplasts by using a density gradient centrifugation method, inoculating the obtained single karyoplasts in a Chang complete culture medium at an inoculation density of single karyoplasts of 1*10<5> to 1*10<6> / ml, and culturing in a culture tank which is at 37 DEG C and saturated humidity and contains CO2 at a volume percentage concentration of 5 percent; 5) amplifying and purifying cells; and 6) storing cells by freezing. When the method of the invention is used, a large number of high-purity target cells, namely endometrial stem cells, can be obtained.
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Description

Technical field

[0001] The present invention belongs to the related technology of cell separation, culture and preservation, especially separation of endometrial mesenchymal stem cells from menstrual blood, establishment of endometrial stem cell strains, and corresponding culture and preservation techniques. Background technique

[0002] Stem cell technology is one of the most popular technologies in life sciences today. Its research content covers almost all biomedical fields of life sciences. In addition to playing an important role in cell therapy, tissue / organ transplantation and gene therapy, it is also discovering new genes , Gene function analysis, developmental biology models and new drug development. In recent years, major breakthroughs have been made in the research of stem cells. In 1999 and 2000, the world's most authoritative American magazine "Science" listed the stem cell and human genome project as the top 10 scientific breakthroughs of the year, especially in 199...

Claims

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