Method for predicting susceptibility of coronary heart disease and kit thereof

A technology for susceptibility and coronary heart disease, applied in the fields of medical biotechnology and genetic diagnosis, can solve the problems of low sensitivity, long time, poor repeatability, etc., and achieve high sensitivity and specificity, easy operation, and stable results
CN102154445AInactive Publication Date: 2011-08-17TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
Publication Date
2011-08-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for predicting the susceptibility of coronary heart disease and a kit thereof. The method comprises the following steps of: 1, picking up the genome DNA of human peripheral blood leukocytes; 2, for the polymorphic locus, namely 396D / I of the DDAH1 gene in a tested individual, which is shown as SEQ ID 1 in a sequence table, specifically amplifying the polymorphic locus from the DNA by F and R oligonucleotide primer sequences shown as the SEQ ID 2 and 3 in the sequence table, and detecting different genotypes of the DDAH1 gene, namely 396 focus by using the Taqman probe base sequence shown as SEQ ID 4 and 5 in the sequence table; and 3, introducing the I / I, D / I and D / D genotypes of the DDAH1 gene, namely 396 focus of the tested individual into a non-conditional Logistic regression analysis model to predict the susceptibility of coronary heart disease. By the method, through the specific combination of the probe and different genotypes of the polymorphic locus, the different genotypes are detected.
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Description

technical field

[0001] The present invention relates to a method for predicting the susceptibility of coronary heart disease (coronary artery disease, CAD), specifically by measuring human dimethylarginine dimethylamine hydrolase 1 (DDAH1) gene polymorphism site -396D / I is used to predict the susceptibility of the population to coronary heart disease. This method can be used for auxiliary diagnosis, treatment, and new drug development of diseases, and belongs to the field of medical biotechnology and genetic diagnosis. Background technique

[0002] In recent years, the morbidity and mortality of coronary heart disease in my country have been rising rapidly, and it has become an important disease that has risen the fastest among the causes of death among Chinese residents and threatens the public health of our country. Traditional risk factors such as hypertension, diabetes, overweight and obesity, smoking and drinking, hyperlipidemia, etc. can cause atherosclerosis, but man...

Claims

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