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Method for extracting and separating L-phenylalanine by utilizing combined technology of membrane separation and industrial chromatographic separation

A technology of phenylalanine and industrial chromatography, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve problems affecting product quality and yield, increase resin regeneration times, increase operating costs, etc., to achieve Effects of reduction of ionic impurities and pigment content, improvement of yield and quality, and reduction of production cost

Inactive Publication Date: 2011-08-24
WUXI JINGHAI AMINO ACID
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The general process of this process is as follows: the fermentation broth is filtered to remove the bacteria, and the separation and extraction of L-phenylalanine from the fermentation broth by this process has the following problems: one is to use the method of filtration to remove the bacteria. Second, the color value of the L-phenylalanine fermentation broth is relatively high, the protein content is high, and the decolorization of ordinary activated carbon is not complete and the dosage is large, which affects the product quality and yield; the third is the L-phenylalanine fermentation broth There are many impurity components and high content in the medium, and the direct use of ion exchange method will seriously pollute the resin, which will easily cause the damage of the resin structure and lose the regeneration ability, which will increase the operating cost. At the same time, the high content of ionic substances will quickly saturate the resin and increase the resin Regeneration times, resulting in large sewage discharge and other problems
Membrane separation technology has been used in the separation and purification of fermentation products. There have been successful precedents, but there is no report on the separation and extraction of L-phenylalanine from fermentation broth using membrane separation and industrial chromatographic separation technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 4L of L-phenylalanine fermentation broth was filtered through a microfiltration membrane with a molecular weight cut-off of 200,000 to remove bacteria, the operating pressure was 0.3MPa, the temperature was 30°C, and the volume of dialysis water was 1L. The filtrate completely removes the bacterial cells; then it is separated by a 1L industrial chromatographic column with a water consumption of 5 liters and a temperature of 30°C to obtain the separated L-phenylalanine supernatant. The separated supernatant was filtered through a sodium filter membrane with a molecular weight cut-off of 500D to remove small molecular impurities and pigments. The operating pressure was 1.2MPa, the temperature was 30°C, the dialyzed water volume was 1.5L, and then it was pre-concentrated through RO reverse osmosis membrane. The results are shown in Table 1 below.

[0026] Table 1

[0027]

Embodiment 2

[0029] 300L of L-phenylalanine fermentation broth was filtered through a microfiltration membrane with a molecular weight cut-off of 800,000 to remove bacteria, the operating pressure was 0.35MPa, the temperature was 30°C, and the dialyzed water volume was 90L. The filtrate completely removes the bacterial cells; then it is separated by an 80L industrial chromatographic column, with a water consumption of 500 liters and a temperature of 30°C to obtain the separated L-phenylalanine supernatant. The separated supernatant was filtered through a sodium filter membrane with a molecular weight cut-off of 500D to remove small molecular impurities and pigments. The operating pressure was 1.2MPa, the temperature was 30°C, the volume of dialysis water was 150L, ​​and then it was pre-concentrated through RO reverse osmosis membrane. The results are shown in Table 2 below.

[0030] Table 2

[0031]

Embodiment 3

[0033] L-phenylalanine fermentation broth 4.5M 3 Bacteria are removed by microfiltration with a molecular weight cut-off of 800,000. The operating pressure is 0.4MPa, the temperature is 30°C, and the volume of dialysis water is 1.5 tons. The filtrate completely removes the bacteria; then passes through 1M 3 The industrial chromatographic column separation, the water consumption is 7 tons, the temperature is 30 ℃, and the separated L-phenylalanine supernatant is obtained. The separated supernatant was filtered through a sodium filter membrane with a molecular weight cut-off of 1000D to remove small molecular impurities and pigments. The operating pressure was 1.2MPa, the temperature was 30°C, the volume of dialysis water was 1.5 tons, and then it was pre-concentrated through RO reverse osmosis membrane. The results are shown in Table 3 below.

[0034] table 3

[0035]

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PUM

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Abstract

The invention relates to a method for extracting and separating L-phenylalanine by utilizing the combined technology of membrane separation and industrial chromatographic separation, belonging to the field of biochemical engineering. The method comprises the following steps: (1) removing thalli from fermentation liquor; (2) performing industrial chromatography efficient separation of inorganic salt and other amino acids; (3) removing impurities and pigment from fermentation liquor; and (4) concentrating fermentation liquor to crystallize, and performing other steps. The method is characterized by mild conditions, simple operations, fewer separation steps, good selectivity and clean production method and overcomes the defects of the prior art, namely low yield, large amount of effluent and high production strength; and the yield and quality of L-phenylalanine can be obviously increased.

Description

technical field [0001] The invention relates to a method for extracting and separating L-phenylalanine, in particular to a method for separating and extracting L-phenylalanine from a fermentation liquid by using a combined technology of membrane separation and industrial chromatographic separation, and belongs to the field of biochemical industry. Background technique [0002] L-phenylalanine, as one of the eight essential amino acids for the human body, is widely used in the pharmaceutical and food additive industries. It is an important component of compound amino acid infusion. In the food processing industry, L-phenylalanine can be added to baked goods. In addition to strengthening the nutritional effect of phenylalanine, it also undergoes amino-carboxylation with sugars. Response to improve food aroma, and to supplement the amino acid balance of functional food needed by the human body. In the pharmaceutical industry, L-phenylalanine is an intermediate of some amino ac...

Claims

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Application Information

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IPC IPC(8): C07C229/36C07C227/40
Inventor 宁健飞蔡立明
Owner WUXI JINGHAI AMINO ACID