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Tissue culture propagation method for curcuma soloensis

A technology of Solomon turmeric and tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve problems such as low reproduction coefficient and difficulty in expanding production scale, and achieve good growth, strong resistance, and robust test-tube seedlings. Effect

Inactive Publication Date: 2012-10-10
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of rhizomes for propagation is not only limited by seasons, but also has a low reproduction coefficient. It can only obtain a multiplication rate of 2-3 times per year. It is difficult to expand the production scale, so it is difficult to occupy a place in the flower market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Excavate the rhizome of Solomon turmeric from the field, remove the roots and leaves, rinse it with tap water, soak it in 0.1% carbendazim solution for 20 minutes, take it out and embed it in wet sand, and store it at 28°C Cultivate under dark conditions for 15 days, and the buds on the stems will germinate immediately to form new shoots of 5-7 cm. Cut off the leaves of the sprouts, keep the sprouts with a length of 0.5-2 cm as explants, soak them in alcohol with a volume fraction of 75% for 20 seconds, and then rinse them once with sterile water. After washing, use tweezers to clamp the sprouts out 0.1% mercuric chloride solution for disinfection for 15 minutes, rinsed with sterile water 8 times, after washing, inoculate the buds one by one into the bud induction medium for cultivation, the cultivation conditions are temperature 26°C, illuminance 1500lx, light The time is 12 hours / day. Adventitious buds can be induced after 30 days of culture. The height of the buds is...

Embodiment 2

[0031]Excavate the rhizome of Solomon turmeric from the field, remove the roots and leaves, rinse it with tap water, soak it in a 0.2% carbendazim solution for 10 minutes, take it out, and bury it with wet sand. Cultivate in a dark environment for 10 days, and the buds on the stems will germinate immediately to form new shoots of 5-7 cm. Cut off the leaves of the new buds, keep the buds with a length of 0.5-2 cm as explants, soak them in 70% alcohol for 30 seconds, and then rinse them with sterile water once. After washing, use tweezers to clamp the buds out Inject 0.2% mercuric chloride solution for disinfection for 10 minutes, rinse 8 times with sterile water, and inoculate the buds one by one into the bud induction medium for cultivation. 24 hours / day, after 20 days of cultivation, adventitious buds are induced, the height of the buds is about 3-5 cm, cut out and transferred to the bud induction medium for subculture proliferation, the culture conditions are temperature 30°...

Embodiment 3

[0035] Excavate the rhizome of Solomon turmeric from the field, remove the roots and leaves, rinse it with tap water, soak it in 0.15% carbendazim solution for 15 minutes, take it out and embed it in wet sand, and store it at 29°C Cultivate in a dark environment for 13 days, and the buds on the stems will germinate immediately to form new shoots of 5-7 cm. Cut off the leaves of the new buds, keep the buds with a length of 0.5-2 cm as explants, soak them in 75% alcohol for 25 seconds, and then rinse them with sterile water once. After washing, use tweezers to clamp the buds out and place The volume fraction is 10% sodium hypochlorite solution for disinfection for 20 minutes, rinsed with sterile water 4 times, after washing, the buds are inoculated one by one into the bud induction medium for cultivation, the cultivation conditions are temperature 28°C, illuminance 1800lx, light time 18 hours Adventitious buds were induced after 25 days of cultivation, and the height of the buds...

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PUM

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Abstract

The invention discloses a tissue culture propagation method for efficiently propagating curcuma soloensis. The curcuma soloensis plants are obtained by using totipotency of plant tissue cells and a plant tissue culture technology through material selection of external plants, sterilization, isolated culture, transplantation from bottles and daily culture management. By using the method, seeds canbe quickly propagated, the propagation speed can be increased and a large amount of test tube plantlets can be obtained, and low investment and high yield can be achieved; the test tube plantlets arerobust, have developed roots, and can be directly transplanted from the bottles without seedling adaptation; the survival rate can reach 100 percent; the test tube plantlets have quick emergence, high stability, robustness, good quality, strong resistance and good growth, and can flower in 6-8 months; and the method provides an effective path for meeting the requirements for a curcuma soloensis seedling market and a flower finished product.

Description

Technical field: [0001] The invention relates to a propagation and cultivation method of Solomon curcuma longa, in particular to a method for propagating Solomon curcuma longa using tissue culture technology. Background technique: [0002] Solomon turmeric (Curcuma soloensis Voel.), belonging to the genus Curcuma in the family Zingiberaceae, is a perennial bulbous herbaceous flower. Native to the Solomon Islands, it has been introduced and cultivated in Guangdong, my country, and performed well when planted in South China. [0003] The plant height of Solomon turmeric is 30-80 cm, the plants are clustered, the leaves are oblong, green, and can be potted or ground as a foliage plant. The flowering period is from April to October. The cylindrical spikes are extracted from the rhizomes. The inflorescence has dense bracts, the upper bracts are purple-red, and the lower bracts are green; It can be used as flower border and flower bed arrangement. In addition, Solomon turmeric ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 吴国江张施君刘念盛爱武
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI