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Oligogalacturonide preparation method

A technology for oligogalactose and uronic acid, applied in the field of preparation of oligogalacturonic acid, can solve the problems of high separation cost, low separation amount, small amount of one-time preparation, etc., and achieves low separation cost, good separation effect, Simple and easy to operate effect

Inactive Publication Date: 2011-09-21
NORTHWEST UNIV
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Problems solved by technology

[0005] But above-mentioned method needs special pectinase in the process of preparation and separation of pectin oligosaccharides, the higher price ion-exchange chromatographic column or preparative high-performance liquid chromatography, separation cost is high, one-time preparation amount is little, and separation amount is low, above These separation methods cannot economically prepare and isolate pectin oligosaccharides

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  • Oligogalacturonide preparation method
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  • Oligogalacturonide preparation method

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Embodiment Construction

[0033] Below in conjunction with specific embodiment this method is described further:

[0034] The invention provides a method for preparing oligogalacturonic acid, comprising the following steps:

[0035] 1) Preparation of pectic acid:

[0036] First, extract pectin polysaccharides according to the existing extraction technology, for example, apple pectin polysaccharides can be extracted from apple peel or citrus, that is, after washing the fresh apple peel, heat it to 90°C for 10-15 minutes to inactivate the enzyme , After filtering through gauze and rinsing several times with warm water at about 50°C until the water is colorless. After crushing the treated apple peel, adjust the pH value to about 1.5-2.0 with HCl under the condition of solid-liquid ratio of 1:20, and extract at 85° C. for 1.5 h. Filtrate while hot and wash the filtrate 2-3 times with warm water to obtain a pectin extract, centrifuge to get the supernatant and concentrate. Protein removal by Savage metho...

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Abstract

The invention provides an oligogalacturonide preparation method which comprises the following steps: 1) preparing pectic acid; 2) preparing a pectin-oligosaccharide mixture by using the pectic acid as the substrate through enzymolysis; and 3) preparing the oligogalacturonide. The invention provides a low-cost, simple and practicable method which can be used for preparing the oligogalacturonide from the pectin-oligosaccharide mixture and can ensure the purity of the oligogalacturonide.

Description

technical field [0001] The present invention relates to a preparation method of oligogalacturonic acid, in particular to a method of using strong basic anion exchange resin as filler to separate pectin-oligosaccharide mixture by gradient elution to obtain oligosaccharides with a polymerization degree of 2-5. Polygalacturonic acid method. Background technique [0002] Pectin oligosaccharides refer to a class of oligosaccharides composed of 2 to 20 monosaccharide units obtained by chemical or enzymatic degradation of pectin. Studies in recent years have shown that pectin oligosaccharides, especially oligogalacturonic acid, have a variety of biological activities, and can be used as inducers of plant defense responses to induce plant resistance responses; they can be used as bifidobacteria proliferation factors, and are highly effective , It specifically promotes the growth of bifidobacteria and improves the structure of the flora; in addition, it has remarkable effects in con...

Claims

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Application Information

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IPC IPC(8): C07H3/06C07H1/08C12P19/14
Inventor 王仲孚黄琳娟
Owner NORTHWEST UNIV
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