Production method for improving validamycin fermentation level

A technology of fermentation level and production method, applied in the field of bioengineering, can solve problems such as affecting the growth of mycelium, affecting the growth and metabolism of bacteria, and abnormal fermentation, so as to ensure normal growth and metabolism, prolong life cycle, and improve fermentation level. Effect

Inactive Publication Date: 2011-09-21
ZHEJIANG QIANJIANG BIOCHEMICAL CO LTD
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, if the concentration of the material in the culture medium is too high and the material liquid is too viscous, it will not only increase the energy consumption of stirring power, but also bring about difficulties in sterilization, which will cause the fermentation to be contaminated by bacteria and lead to fermentation failure, causing great harm to large-scale production. On the other hand, the high concentration of materials in the medium will cause the problem of high osmotic pressure, which will inhibit and affect the growth of the mycelium in the early stage, and affect the normal growth and metabolism of the bacteria, resulting in fermentation abnormal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The effect of supplementing cornstarch liquefied liquid on the fermentation cycle and fermentation level was investigated in a 2300L fermenter. The mature seed culture solution was transplanted into the sterilized fermentation medium in the fermenter for cultivation, and when the total sugar concentration dropped below 4.0% by mass percentage, it was supplemented every 6 hours with a mass percentage of 35%. % of cornstarch liquefaction solution, the supplementary volume is 15-30L each time, the implementation steps and results are as follows.

[0017] ⑴Seed cultivation:

[0018] A 250L tank is used as a seed liquid culture tank, and the seed medium is: 6kg rice flour, 3kg peanut cake powder, 1kg yeast powder, 20g potassium dihydrogen phosphate, 30g sodium chloride, 75g soaked enemy, add water to volume 130L, pH before elimination Naturally, sterilize at 121°C for 30 minutes, cool to 40°C after sterilization, insert the spore suspension in two 250ml spore bottles, and c...

Embodiment 2

[0027] The effects of adding rice flour liquefied liquid on the fermentation cycle and fermentation level were investigated in a 2300L fermenter. The mature seed culture solution was transplanted into the sterilized fermentation medium in the fermenter for cultivation, and when the total sugar concentration dropped below 4.0% by mass percentage, it was supplemented every 6 hours with a mass percentage of 35%. % of rice flour liquefaction solution, the amount added each time is 15-30L, the implementation steps and results are as follows.

[0028] (1) Seed culture: with embodiment 1.

[0029] (2) Preparation of rice flour liquefaction liquid with a mass percentage of about 35%:

[0030] Use a 250L tank as a carbon source feeding tank, put in 60kg of rice flour, add water to 140L, stir, add 0.4kg of calcium chloride, adjust the pH to 6.2 with liquid caustic, add 400g of medium temperature a-amylase (enzyme activity: 2000u / g), then raise the temperature to 80°C and keep it warm...

Embodiment 3

[0037] at 40m 3 The effect of adding rice flour liquefaction liquid on the fermentation tank on the fermentation cycle and fermentation level was investigated. The mature seed culture solution was transplanted into the sterilized fermentation medium in the fermenter for cultivation, and when the total sugar concentration dropped below 4.0% by mass percentage, it was supplemented every 6 hours with a mass percentage of 35%. % of rice flour liquefaction solution, the volume of each addition is 300-600L, a total of 4 times, the implementation steps and results are as follows.

[0038] ⑴Seed cultivation:

[0039] A 7000L tank is used as a seed liquid culture tank, and the seed medium is: 160kg of rice flour, 80kg of peanut cake powder, 30kg of yeast powder, 0.6kg of potassium dihydrogen phosphate, 0.8kg of sodium chloride, 1.5kg of distilled water, and 3700L of water. Before sterilization, the pH is natural, sterilize at 121°C for 30 minutes, cool to 40°C after sterilization, in...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a production method for improving validamycin fermentation level, comprising the following steps: firstly, inoculating a spore fluid suspension prepared in a bacteria room into a sterilized seed culture medium in a seeding tank to obtain a matured seed nutrient solution; secondly, transplanting the matured seed culture solution by the differential pressure method into a disinfected fermentation culture medium in a fermentation cylinder for fermentation, during which a proper amount of carbon sources is added into the fermentation culture medium by the fed-batch method or in batches to accomplish validamycin ferment production if the total sugar concentration is lower than 4.0%. According to the invention, carbon source breed, supplement time and supplement modes are clear. According to supplement amounts of different carbon sources, the fermentation period can be prolonged from originally about 40 hours to 65-100 hours and the fermentation level is improved from originally 16-18g / L (ingredient A) to above 25g / L (ingredient A), thus greatly improving the validamycin fermentation level.

Description

technical field [0001] The invention relates to a method in the technical field of bioengineering, in particular to a production method for improving the fermentation level of Jinggangmycin. Background technique [0002] Jinggangmycin, also known as effective mycin abroad, is a water-soluble aminoglycoside antibiotic produced by Streptomyces hygroscopicus. It has the characteristics of high efficiency, low toxicity, no residue, etc., is friendly to the environment, and has no fungal drug resistance. At present, the area used in agricultural production in my country has reached 200 million mu, and it is the most widely used agricultural antibiotic in my country. At the same time, it is also the direct raw material for the production of two clinical medicines for diabetes, acarbose (Baisupine) and voglibose (Bexin). [0003] During the nearly 40 years of industrial production of Jinggangmycin, most people improved the fermentation level of Jinggangmycin through strain transf...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P19/46C12R1/55
Inventor 裘国寅常宏
Owner ZHEJIANG QIANJIANG BIOCHEMICAL CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap