Preparation method of bacteria cellulose/hyaluronic acid composite

A technology of bacterial cellulose and bacterial cellulose membrane, which is applied in the field of crosslinking, can solve the problem of not realizing the transformation of membrane matrix material nutrition control release skin layer damage repair function

Active Publication Date: 2011-10-12
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these work reports all use pure bacterial cellulose as the membrane material, and have not realized the functional transfor

Method used

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  • Preparation method of bacteria cellulose/hyaluronic acid composite
  • Preparation method of bacteria cellulose/hyaluronic acid composite

Examples

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preparation example Construction

[0016] With reference to the drawings, the preparation method of the bacterial cellulose / hyaluronic acid composite material of the present invention includes the following steps:

[0017] In the first step, the activated Acetobacter xylinum strain is connected to the seed culture solution for shaking culture, the seed culture solution is connected to the fermentation culture solution, shaken, and then statically cultured at a culture temperature of 26-32°C. The amount of seed liquid is 1%-10%, and the bacterial cellulose membrane is prepared;

[0018] In the second step, the bacterial cellulose membrane prepared in the second step is rinsed with water and then placed in hot water. The temperature of the hot water is 80-90°C and the standing time is 3 hours. Then the bacterial cellulose membrane is taken out and placed Soak the membrane in an alkaline solution, then neutralize it with an acid buffer solution, soak the bacterial cellulose membrane in deionized water to neutral; the a...

Embodiment 1

[0025] Implementation Example 1: The preparation method of the bacterial cellulose / hyaluronic acid composite material of the present invention includes the following steps:

[0026] The first step is to take the activated Acetobacter xylinum strains and insert them into the seed culture solution, and culture at 29°C for 12 hours with shaking at a constant temperature of 120r·min. -1 . Add 5% of the inoculum to the fermentation broth, shake well, and incubate at a constant temperature of 29°C for one week to prepare a bacterial cellulose membrane.

[0027] The second step is the purification treatment of the product. The cellulose membrane prepared above is taken out and rinsed with tap water several times and then rinsed with deionized water; the rinsed membrane is placed in 80 ℃ water and treated for 3 hours; 1% sodium hydroxide Soak it in the solution, and place the soaking solution in a 40℃ water bath for 60 hours; neutralize the sodium hydroxide with an acetic acid-sodium aceta...

Embodiment 2

[0032] Implementation Example 2: The preparation method of the bacterial cellulose / hyaluronic acid composite material of the present invention includes the following steps: the first step is to take the activated Acetobacter xylinum strain into the seed culture solution, culture at 30°C with constant shaking for 8 hours, shake Bed speed 200r·min -1 . Add 3% of the inoculum to the fermentation broth, shake well, and incubate at a constant temperature of 30°C for one week to prepare a bacterial cellulose membrane.

[0033] The second step is the purification treatment of the product. The cellulose membrane prepared above is taken out and rinsed with tap water for several times and then rinsed with deionized water; the rinsed membrane is placed in 90℃ water and treated for 3 hours; 2% sodium carbonate solution Soak it and place the soaking solution in a water bath at 50°C for 48h; neutralize sodium hydroxide with a pH 4.5 acetic acid-sodium acetate buffer solution; soak the cellulos...

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Abstract

The invention discloses a preparation method of a bacteria cellulose/hyaluronic acid composite. The functional material is prepared according to the following steps of: pre-treating a bacteria cellulose wet film, impregnating slices of the bacteria cellulose wet film in a hyaluronic acid solution, and then freezing at low temperature and drying the hyaluronic acid-adsorbing bacteria cellulose slice to remove a certain moisture. The bacteria cellulose/hyaluronic acid composite is formed by means of crosslinking cellulose molecules and the hyaluronic acid under the action of carbodiimide. Through the crosslinking, not only is the stability of the hyaluronic acid improved, but also the wetting property, the air permeability and the biocompatibility of the bacteria cellulose are enhanced. According to the preparation method disclosed by the invention, a certain amount of the hyaluronic acid is crosslinked in the bacteria cellulose film, so that high flexibility and adhesion of the bacteria cellulose as well as the moisturizing, nourishing and skin moistening effects of the hyaluronic acid can be exerted, and the prepared bacteria cellulose/hyaluronic acid composite can be used in the fields, such as healing promoting dressings, self-nutrition type whitening masks, and the like.

Description

Technical field [0001] The invention belongs to the cross-linking technology of modified bacterial cellulose membrane with hyaluronic acid, in particular to a preparation method of bacterial cellulose composite material. Background technique [0002] Wound dressings and facial masks mainly produced from chemically synthesized raw materials have poor affinity with the skin, and the discarded materials after use will also cause pollution to the environment. The use of renewable raw materials, especially natural products, to produce such products is of great significance [0003] Plant cellulose is the most abundant natural high polymer in nature, and its research as a biological material has long attracted attention due to technical issues such as the content of plant cellulose, the extraction and refining of cellulose. Plant cellulose is rare due to research on facial masks. Different from plant cellulose, bacterial cellulose is not a structural component of bacterial cell walls, ...

Claims

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Application Information

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IPC IPC(8): C08L1/02C08L5/08C08J7/12C08J3/24C12P19/04A61L15/28A61Q19/02C12R1/02
Inventor 孙东平汪信何浩明杨加志
Owner NANJING UNIV OF SCI & TECH
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