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Method for extracting immune globulin from yak blood

An immunoglobulin and yak technology, applied in the field of natural medicine extraction, can solve the problems of less research on purification purity and yield, insufficient utilization of yak value, and unutilized blood, etc. less toxic effect

Inactive Publication Date: 2011-11-02
苏州北商智业管理咨询有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the yak is slaughtered for meat, its blood is often not used, and all of it is discharged as sewage, which not only makes the value of the yak unable to be fully utilized, but also increases the environmental burden
[0004] The commonly used methods for extracting immunoglobulin from blood are salting-out method (such as sodium polyphosphate flocculation method, ammonium sulfate salting-out method), organic solvent precipitation method (such as cold ethanol separation method, octanoic acid precipitation method), organic polymer Precipitation, denaturation precipitation, cellulose chromatography, gel chromatography, ultrafiltration, etc. Many scholars have done some in-depth research on the extraction of immunoglobulins, but there are many comprehensive methods for antibody purification. The bottleneck stage that limits the purification purity and yield—the crude extraction stage is less studied

Method used

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Experimental program
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Effect test

Embodiment 1

[0025] Add 0.3% sodium citrate to 100L of fresh yak blood, centrifuge at high speed to separate the blood, retain the plasma, concentrate it through a porous fiber ultrafiltration membrane with a molecular weight cut-off of 3000-10000D, wash with water, and take the retentate phase. The entrapped phase was ultrasonically extracted with 10 times the amount (V / W) of ethanol for 20 minutes, repeated twice, the extracts were combined, hydrochloric acid was added to adjust the pH to 7.3-7.7, the precipitate was obtained by centrifugation, and freeze-dried to obtain immunoglobulin with a purity of 78.7%.

Embodiment 2

[0027] Add 0.3% sodium citrate to 100L of fresh yak blood, centrifuge at high speed to separate the blood, retain the plasma, concentrate it through a porous fiber ultrafiltration membrane with a molecular weight cut-off of 3000-10000D, wash with water, and take the retentate phase. The entrapped phase was ultrasonically extracted with 15 times the amount (V / W) of ethanol for 30 min, repeated twice, the extracts were combined, hydrochloric acid was added to adjust the pH to 7.3-7.7, the precipitate was obtained by centrifugation, and freeze-dried to obtain immunoglobulin with a purity of 78.4%.

Embodiment 3

[0029] Add 0.3% sodium citrate to 100L of fresh yak blood, centrifuge at high speed to separate the blood, retain the plasma, concentrate it through a porous fiber ultrafiltration membrane with a molecular weight cut-off of 3000-10000D, wash with water, and take the retentate phase. The entrapped phase was ultrasonically extracted with 10 times the amount (V / W) of ethanol for 20 min, repeated twice, the extracts were combined, hydrochloric acid was added to adjust the pH to 7.3-7.7, the precipitate was obtained by centrifugation, and freeze-dried to obtain immunoglobulin with a purity of 79.1%.

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Abstract

The invention relates to a method for extracting immune globulin from yak blood. The method comprises the following steps: adding anticoagulant into fresh yak blood and then centrifuging the mixture, thereby acquiring blood plasma; utilizing an ultra-filtration membrane to purify and concentrate the blood plasma; ultrasonically extracting an interception phase with ethanol; and freeze-drying, thereby acquiring the immune globulin. The method provided by the invention is simple, high-speed, safe, pollution-free, low-cost, and low-carbon.

Description

Technical field: [0001] The invention belongs to the technical field of natural medicine extraction, and relates to a method for extracting immunoglobulin from yak blood. Background technique: [0002] Immunoglobulin (Ig for short) refers to a globulin that has antibody activity and can specifically bind to corresponding antigens, and is commonly found in the blood, tissue fluid, lymph fluid and exocrine fluid of mammals. Immunoglobulin plays an important immune and physiological regulatory role in animals, and is one of the most critical components of the immune system in animals. Immunoglobulins can be divided into five classes: IgG, IgA, IgM, IgD, and IgE. The main component of human serum immunoglobulin is IgG, which accounts for 70-75% of the total immunoglobulin, with a molecular weight of about 150,000 and sugar content of 2-3%. Although immunoglobulins vary widely, they all have similar structures. Antibody molecules are composed of two pairs of polypeptide chains...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/06C07K1/36C07K1/34C07K1/30
Inventor 刘宝岩张翼李法庆
Owner 苏州北商智业管理咨询有限公司
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