Extraction method of bovine serum albumin

A technology of bovine serum albumin and an extraction method, which is applied in the field of preparation of albumin, can solve the problems of difficulty in guaranteeing the purity of albumin, difficulty in mass production, complicated production process, etc., and achieves low cost, easy storage and short process flow. Effect

Inactive Publication Date: 2011-11-16
河南省医药科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the above preparation method of bovine serum albumin, many high-cost salts (such as sodium citrate, sodium octanoate, etc.) and organic chemical substances (ethan

Method used

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Effect test

Embodiment 1

[0021] Embodiment 1 A method for extracting bovine serum albumin, comprising the following steps:

[0022] Fresh bovine blood was taken, centrifuged at 3000r / m for 10min, the supernatant was taken, and frozen at -20°C for later use. Take 30ml of fully melted bovine serum, add 30ml of deionized water, stir well, then add 20ml of 0.024M sodium palmitate solution at 70°C, and let stand for 75min. The pH of the mixed solution was measured to be 8.76, and 1.6ml of 1M HCl was added to bring the pH to 5.16, and the water bath was 1.5h (the temperature rose from 65°C to 70°C). Filter with a single layer of ordinary filter paper to obtain 60ml of filtrate, add 12ml of 3M HCl, 2.88g of NaCl, place at 4°C for 30min, use double-layer filtration, add 1.7ml of water to the precipitate, stir it into a paste, add 3.5ml of 1M NaOH, pH to 9.98, add 0.11ml of 1M HCl to pH8.45, and dialyze in deionized water for 12h. Put 13.5ml of the dialysate into a freeze dryer, and take it out after 48 hour...

Embodiment 2

[0023] Embodiment 2 A method for extracting bovine serum albumin, comprising the following steps:

[0024] Take fresh bovine blood, centrifuge at 3000r / m for 10min, take the supernatant, and freeze it at -20°C for later use.

[0025] Take 30ml of fully melted bovine serum, add 30ml of deionized water, stir well, then add 20ml of 0.024M sodium palmitate solution at 70°C, and let stand for 75min. The pH of the mixed solution was measured to be 8.78, and 1.7ml of 1M HCl was added to bring the pH to 5.17, and the water bath was 1.5h (the temperature rose from 65°C to 70°C). Filter with single-layer filter paper to obtain 60ml of filtrate, add 12ml of 3M HCl, 2.88g of NaCl, place at 4°C for 30min, use double-layer filtration, add 1.5ml of water to the precipitate, stir into a paste, add 3.0ml of 1M NaOH, pH to 10.04 , and then add 0.20ml of 1M HCl to pH8.51, and dialyze in deionized water for 12h. Put 12.5ml of the dialysate into the freeze dryer and take it out after 48 hours to...

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Abstract

The invention relates to an extraction method of bovine serum albumin, aiming to solve the technical problems of complexity, low purity, high production cost and difficulty in large-lot production of the production process of bovine serum albumin. The extraction method comprises the following steps of: adding a proper amount of sodium palmitate to the separated serum, standing, regulating the pH of the solution, heating in water bath for 1-2 hours, filtering, sequentially adding 3MHCl and sodium chloride to the filtrate, standing at 4 DEG C, filtering to obtain precipitate, adding a proper amount of water to the precipitate, regulating the pH for dissolving the precipitate, then regulating the pH of the solution to 8.0-9.0, dialyzing and lyophilizing. The extraction method of bovine serumalbumin has low process materials input, short process flow, simplicity of operation, high production efficiency and suitability for mass production, and the obtained product has high purity and goodquality and is easy to preserve.

Description

technical field [0001] The invention relates to a method for preparing albumin, in particular to a method for extracting bovine serum albumin. Background technique [0002] Bovine serum albumin (BSA), also known as the fifth component, is a globulin in bovine serum, containing 583 amino acid residues, with a molecular weight of 66.430 kDa and an isoelectric point of 4.7. , biochemical reagents widely used in various fields of pharmacy, and can also be used as medical and health food and condiment. [0003] The most commonly used preparation method of bovine serum albumin is to separate the serum from bovine blood, use ammonium sulfate for fractional precipitation, and obtain it through caprylic acid treatment; The method is to use 3.8% sodium citrate to anticoagulate blood to obtain plasma, heat and add sodium caprylate, add ethanol after adjusting the pH value, and then obtain bovine serum albumin after filtering, desalting, concentrating and freeze-drying. In the above p...

Claims

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Application Information

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IPC IPC(8): C07K14/765C07K1/14
Inventor 何美霞贺石林华海婴李志刚张黎蓉张红梅王芳张勇高元法崔西国丁利静
Owner 河南省医药科学研究院
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