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Pharmaceutical preparation comprising supernatant of blood mononuclear cell culture

A supernatant, nuclear cell technology, used in drug combinations, surgical drugs, antipyretics, etc., can solve problems such as tissue damage that does not directly solve

Active Publication Date: 2011-11-23
APOSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other drugs, including digoxin, diuretics, amrinone, beta-blockers, lipid-lowering agents, and angiotensin-converting enzyme inhibitors, are used to stabilize the disease, but none of these treatments directly address the Tissue damage from ischemia and hypoxia

Method used

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  • Pharmaceutical preparation comprising supernatant of blood mononuclear cell culture
  • Pharmaceutical preparation comprising supernatant of blood mononuclear cell culture
  • Pharmaceutical preparation comprising supernatant of blood mononuclear cell culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1: Culture supernatants of peripheral blood mononuclear cells strongly enhance wound healing.

[0076]Unhealed skin ulcers are often resistant to most common treatments. Previous studies have shown that the administration of peripheral blood mononuclear cells (PBMCs) together with basic fibroblast growth factor is a useful treatment for diabetic gangrene. In this example it was investigated whether culture supernatants of PBMCs (non-irradiated or irradiated) were sufficient to induce enhanced wound healing in a mouse model. Furthermore, the effect of these supernatants on human primary fibroblasts (FB), keratinocytes (KC) and endothelial cells (EC) was analyzed.

[0077] Incubation of FB and KC with PBMC-derived supernatants revealed that both non-irradiated and irradiated cell supernatants strongly induced FB migration, but they had no effect on FB proliferation. In contrast, both supernatants were shown to be effective on the migratory and proliferative ca...

Embodiment 2

[0079] Example 2: Quiescent Peripheral Blood Mononuclear Cells (PBMCs) Demonstrate Low Activation Markers and Reduced Inflammatory Cells cytokine production

[0080] Activated peripheral blood mononuclear cells (PBMC) and their supernatant (SN) are thought to be beneficial in wound regeneration (Holzinger C et al. Eur J Vasc Surg. 1994 May;8(3):351-6). In Examples 1 and 2, it was shown that unactivated PBMCs and their derived SNs have beneficial effects in experimental acute myocardial infarction (AMI) and trauma models. Since non-activated PBMCs had to be confirmed experimentally, it was investigated whether culture of PBMCs resulted in enhanced T cell activation markers (CD69, CD25) or enhanced secretion of inflammatory cytokines (monocyte activation = TNFα, T cell activation =INFγ). In control experiments, cultured T cells were stimulated by CD3 mAb stimulation or phytohemagglutinin (PHA).

[0081] Method and Results

[0082] Venous blood was collected from healthy...

Embodiment 3

[0091] Example 3: Proliferative Activity of PBMCs Cultured in Physiological Solution

[0092] The purpose of this example was to demonstrate that specific (CD3), non-specific (lectin, PHA) and allogeneic T cells were utilized in comparison to 2-day (CD3, PHA) and 5-day (MLR) stimulation assays Triggered (Mixed Lymphocyte Reaction, MLR) immunoassay, PBMC had no proliferative activity.

[0093] Materials and methods

[0094] PBMCs were isolated from young healthy volunteers by Ficoll density gradient centrifugation and 1*10 per 200 μL 5 Each cell was resuspended in RPMI (Gibco, USA) containing 0.2% gentamicin sulfate (Sigma Chemical Co, USA), 1% L-glutamine (Sigma, USA). Responder cells were treated with anti-CD3 MoAb (10 μg / mL, BD, NJ, USA), PHA (7 μL / mL, Sigma Chemical Co, USA), or with irradiated allogeneic PBMC at a 1:1 ratio (for MLR )Stimulate. Incubate the plate for 48h or 5 days, then use 3 [H]-thymidine pulse 18h (3.7*10 4 Bq / well; Amersham Pharmacia Biotech, ...

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Abstract

The present invention relates to a topical pharmaceutical preparation for treating an inflammatory skin condition, preferably a condition associated with ischemia, comprising a supernatant of a physiological solution obtainable by cultivating peripheral blood mononuclear cells (PBMCs) or a subset thereof in a physiological solution free of PBMC-prolif erating and PBMC-activating substances for at least 1 h.

Description

technical field [0001] The present invention relates to a pharmaceutical formulation for the treatment of inflammatory skin diseases in vivo, preferably skin diseases associated with ischemia in vivo. Background technique [0002] Hypoxia, a condition of low oxygen, may occur when the lungs are damaged or blood flow is reduced. Ischemia, a reduction in blood flow, may be caused by a blood clot (thrombus) or any external circulatory event (embolus) or vascular disorder such as atherosclerosis that blocks an artery or vein. Decreased blood flow may come on suddenly and be short-lived (acute ischemia) or may be slow-onset and long-lasting or recur frequently (chronic ischemia). Acute ischemia is often accompanied by localized, irreversible tissue necrosis (infarction), while chronic ischemia is usually accompanied by transient hypoxic tissue trauma. However, chronic ischemia can lead to infarction if the decrease in perfusion is prolonged or severe. Infarctions usually occur...

Claims

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Application Information

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IPC IPC(8): A61K35/14A61K35/16A61K35/17
CPCA61K35/14A61K35/16A61K35/17A61P17/00A61P17/02A61P29/00A61P41/00A61P9/10A61K39/46433A61K39/461A61K39/4621
Inventor H·J·安科斯密特
Owner APOSCI
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