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Method for freezing and concentrating marine bioactive substances

A technology of freeze concentration and marine biology, which is applied in chemical instruments and methods, preparation of organic compounds, preparation of cyanide reaction, etc. It can solve problems such as poor appearance and color, increased separation cost, and fishy and bitter taste, so as to avoid membrane fouling Problems and solutions to the effect of protein denaturation

Inactive Publication Date: 2011-12-07
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The color and flavor of liquid products obtained by traditional separation methods such as screen filtration and thermal concentration methods are not good, and precipitation and stratification are often prone to occur during placement. Powder products made from solutions containing active substances often have Rough powder, poor appearance and color, fishy and bitter taste, easy to absorb moisture and agglomerate
Membrane separation technology is much more efficient in separating marine biologically active substances than traditional methods, but the effect is not good when applied to industrial production, and the membrane is easily polluted, which greatly increases the cost of separation
Secondly, during the membrane separation process, some enzyme activities may be affected under the action of high-speed shear force

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Take 5 kg of aquatic raw materials with fresh abalone organ protein as the main component.

[0013] (2) After adding water and mixing, add protease to enzymatically hydrolyze to obtain abalone viscera protein hydrolyzate, the total solid content is 5%, and the amino acid content accounts for 48% of the total solid content.

[0014] (3) The abalone viscera protein hydrolyzate was frozen and concentrated, and the temperature of the refrigerant dropped to -18°C, at which point the ice crystals continued to grow.

[0015] (4) Ice crystals are separated, and the separated liquid is poured back into the concentration tank to freeze and concentrate until the total solid content reaches 13%. At this time, the amino acid content in the solution accounts for 64% of the total solid content, and the small molecule amino acids are purified and concentrate.

Embodiment 2

[0017] (1) Take 5 kilograms of protein raw materials obtained after removing bones and grease from fresh herring and herring scraps.

[0018] (2) After adding water and mixing, add protease to enzymatically hydrolyze to obtain medlar protein hydrolyzate, the total solid content is 5.5%, and the amino acid content accounts for 51% of the total solid content.

[0019] (3) Freezing and concentrating the hydrolyzate of medlar protein, the temperature of the refrigerant drops to -25°C, and the ice crystals continue to grow at this time.

[0020] (4) Ice crystals are separated, and the separated liquid is poured back into the concentration tank to freeze and concentrate until the total solid content reaches 13.5%. At this time, the amino acid content in the solution accounts for 72% of the total solid content, and the small molecule amino acid is purified and concentrate.

Embodiment 3

[0022] (1) Take 5 kg of shrimp protein raw material obtained after removing oil from fresh shrimp heads and shells.

[0023] (2) After adding water and mixing, add protease to hydrolyze to obtain shrimp protein hydrolyzate, the total solid content concentration is 5.8%, and the amino acid content accounts for 50% of the total solid content.

[0024] (3) The shrimp protein hydrolyzate is frozen and concentrated, and the temperature of the refrigerant drops to -35°C, and the ice crystals continue to grow at this time.

[0025] (4) Ice crystals are separated, and the separated liquid is poured back into the concentration tank to freeze and concentrate until the total solid content reaches 13.9%. At this time, the amino acid content in the solution accounts for 78% of the total solid content, and the small molecule amino acids are purified and concentrate.

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Abstract

The invention relates to a method for freezing and concentrating and separating marine biological active substances. The method is to hydrolyze aquatic raw materials to obtain aquatic protein hydrolyzate, which contains small molecular amino acids, polypeptides, proteins, polysaccharides and other macromolecular active substances, and then Aquatic protein hydrolyzate is processed by freezing and concentrating, taking advantage of the different diffusion speeds of small molecular amino acids and macromolecules such as peptides, proteins, and polysaccharides in the liquid. The small molecular amino acids diffuse quickly and remain in the liquid and are concentrated. 1. The diffusion speed of polysaccharide is slow, and it is wrapped or entrained by ice crystals in the process of freeze concentration and crystallization, so that it can be separated. The invention uses freeze concentration for the separation of marine biological active substances, can concentrate and separate the hydrolyzate of aquatic protein at low temperature, maintain the biological activity and flavor of the hydrolyzate to the greatest extent, solve the problem of protein denaturation caused by heat concentration, and avoid membrane separation. Membrane fouling, and the effect on the activity of some enzymes under the action of high-speed shear force.

Description

technical field [0001] The invention belongs to the field of aquatic product processing, and more specifically relates to a method for freezing, concentrating and separating marine biological active substances. Background technique [0002] Polypeptides, proteins, polysaccharides and other macromolecules in marine organisms have complex chemical structures and high biological activities, and are rich resources for health care products and new drug screening. [0003] At present, the research on the separation of macromolecular active substances in aquatic enzymatic hydrolyzate mainly focuses on membrane separation technology. The color and flavor of liquid products obtained by traditional separation methods such as screen filtration and thermal concentration methods are not good, and precipitation and stratification are often prone to occur during placement. Powder products made from solutions containing active substances often have The powder is rough, the appearance is n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07C229/00C07C227/40C08B37/00
Inventor 陈锦权方婷
Owner FUJIAN AGRI & FORESTRY UNIV