A method for extracting malt limit dextrinase

A technology of limit dextrinase and malt, which is applied in the field of extracting malt limit dextrinase, can solve the problems of strict source requirements, restriction of use of bacterial source enzymes, etc., and achieve the effect of high enzyme activity

Inactive Publication Date: 2011-12-07
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, food-grade enzyme preparations have strict requirement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The first step is to dry the barley malt in an oven at 40°C for 24 hours to remove excess moisture;

[0028] In the second step, the above-mentioned barley malt after drying treatment is pulverized by a DLFU disc mill, and passed through a sieve with an aperture of 80 mesh for subsequent use;

[0029] The third step is to add reducing agent L-cysteine ​​hydrochloride in the acetic acid-sodium acetate buffer solution of 0.1mol / L, so that its concentration is 20mmol / L, and the pH value of the buffer solution is adjusted to 5.0;

[0030] The fourth step is to add the extraction buffer prepared in the third step to the malt powder. 20h, then centrifuged in a centrifuge with a rotating speed of 3000r / min for 15min, and the supernatant was taken to obtain a limit dextrinase extract with an enzyme activity of 413mU / g.

Embodiment 2

[0032] The first step is to dry the barley malt in an oven at 35°C for 36 hours to remove excess water;

[0033] In the second step, the above-mentioned barley malt after drying treatment is pulverized by a ZN-08 small pulverizer, and passed through a 40-mesh sieve for subsequent use;

[0034] The third step is to add reducing agent L-cysteine ​​hydrochloride in the 0.1mol / L acetic acid-sodium acetate buffer solution to make its solubility 25mmol / L, and adjust the pH value of the buffer solution to 5.5;

[0035] The fourth step is to add the extraction buffer prepared in the third step to the malt powder. 16h, and then centrifuged in a centrifuge with a rotating speed of 5000r / min for 10min, and the supernatant was taken to obtain a limit dextrinase extract with an enzyme activity of 433mU / g.

Embodiment 3

[0037] The first step is to dry the barley malt in an oven at 38°C for 30 hours to remove excess water;

[0038] The second step is to grind the above-mentioned dried barley malt with a grinding cup, and pass through a 60-mesh sieve for subsequent use;

[0039] The third step is to add reducing agent L-cysteine ​​hydrochloride to the 0.1mol / L acetic acid-sodium acetate buffer solution to make its solubility 15mmol / L, and adjust the pH value of the buffer solution to 6.0;

[0040] The fourth step is to add the extraction buffer prepared in the third step to the malt powder. 12h, and then centrifuged in a centrifuge at 4000r / min for 12min, and the supernatant was taken to obtain a limit dextrinase extract with an enzyme activity of 287mU / g.

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Abstract

The invention discloses a method for extracting malt limit dextrinase. The method includes: 1) crushing the dried barley malt with a pulverizer; 2) passing the crushed malt powder through a 40-80 mesh sieve to obtain pretreated malt powder; 3) adding 15-25mmol/LL - 0.1mol/L acetic acid-sodium acetate buffer solution with a pH value of 5.0~6.0 of cysteine ​​hydrochloride, adjust the solid-liquid ratio to 1:4~1:6g/mL, and place it at a speed of 80~120r/mL min in a water bath constant temperature oscillator, separated and extracted at a temperature of 35 to 45°C for 12 to 20 hours, and centrifuged in a centrifuge with a rotation speed of 3000 to 5000r/min for 10 to 15 minutes to obtain malt limit dextrinase. The limit dextrinase is preliminarily separated by this method, the process is simple, the target enzyme can be dissolved to the greatest extent, the extraction effect is good and the cost is low.

Description

technical field [0001] The invention relates to the field of food biotechnology, in particular to a method for extracting malt limit dextrinase. Background technique [0002] Limit dextrinase (limit dextrinase, EC 3.2.1.4), that is, pullulan 6-glucose hydrolase, is an endo-amylase. It can specifically catalyze the α-1,6 glycosidic bonds in the branch points of pullulan, pullulan, α-limit dextrin, β-limit dextrin and the like. [0003] Due to the high specificity of limit dextrinase to α-1,6 glycosidic bonds, it has been widely used in food, textile, washing and other industries, especially in food industries such as sugar production, starch processing and brewing. It plays an important role. In the sugar industry, the compound use of extreme dextrinase and other amylases can increase the saccharification rate, which has important commercial value; It has good utilization value; in the brewing industry, the fermentability of wort can be improved by adding limit dextrinase,...

Claims

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Application Information

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IPC IPC(8): C12N9/24
Inventor 胡飞冯倩倩
Owner SOUTH CHINA UNIV OF TECH
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