Application of metal derivative containing chlorophyll and hydrolyzate thereof as plant growth regulator
A technology for plant growth regulators and metal derivatives, applied in plant growth regulators, plant growth regulators, chemicals for biological control, etc., can solve the problem of long residual effect, difficult accumulation, and unclear metabolic pathways at high concentrations Chemical properties and other issues, to achieve the effect of stable solution properties, reduced vigor, and excellent plant growth regulation activity
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Embodiment 1
[0031] Example 1: Chlorophyllase inhibitory activity (in vitro test) Accurately weigh 1.00 g of acetone powder made from fresh mung bean leaves, extract with 25 mL of phosphate buffer (pH 7.0-7.5) for 4.5 h, centrifuge at 4°C for 10 min, and take the The clear liquid is the chlorophyll enzyme liquid, the active enzyme concentration: 0.14mmol / L, the substrate chlorophyll concentration: 130ppm. Determination of the chlorophyllase inhibition rate of 0.4ppm~25.0ppm each concentration of chlorophyllase metal derivative sample solution (Gan Zhijun, Wang Xiaoyun, Zou Qi. Research on biochemical kinetic characteristics of wheat chlorophyllase. Northwest Botanical Journal, 2003, 23 (5): 750 ~754.), and calculate the IC 50 Value, Series Sample IC 50 The results are shown in the table below
[0032] Chlorophyllase inhibitory activity IC of a series of samples 50 *
[0033]
[0034] * : Substrate concentration 324ppm (0.36mM)
Embodiment 2
[0035] Example 2: Corn seed soaking germination rate and seedling growth time: April 2009; corn variety: Cuitian No. 1; sample: ferric chlorophyllin and ferric chlorophyllin; test method: 0.001ppm~1.0ppm sample solution Corn seeds were soaked for 18 hours, and the water soaking group was the control (CK), and then transferred to soil with the same soil quality and fertility for cultivation, and watered once every 2 days. Compared with the control after 10 days, examine the difference of its germination rate, plant height and main root length (all are average values), the results are shown in the table below
[0036] Comparison of Germination Rate and Seedling Growth of Soaked Corn Seeds
[0037]
[0038]
[0039] The germination rate, plant height and root length of each concentration of the test group were significantly different from those of CK. The test group not only had good growth, but also had rich and well-developed root system, many lateral roots, and main root...
Embodiment 3
[0040] Example 3: Chlorophyllase inhibition experiment in vivo, select mung bean seeds with plump grains and uniform size and soak them in 0.1 ppm solution of the novel plant growth regulator of the present invention for 4 hours, soak the seeds at a room temperature of 28° C. 0.1ppm solution culture in the box, when 1 to 2 pairs of true leaves grow (about 8 days later), use the leaves to make acetone powder, use water culture as a blank (inhibition rate: 0%), and measure the chlorophyllase activity of each treatment group , get the result in the following table
[0041]
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