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Application of metal derivative containing chlorophyll and hydrolyzate thereof as plant growth regulator

A technology for plant growth regulators and metal derivatives, applied in plant growth regulators, plant growth regulators, chemicals for biological control, etc., can solve the problem of long residual effect, difficult accumulation, and unclear metabolic pathways at high concentrations Chemical properties and other issues, to achieve the effect of stable solution properties, reduced vigor, and excellent plant growth regulation activity

Active Publication Date: 2011-12-14
NANJING NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant growth regulators generally have the characteristics of promoting plant growth at low concentrations and inhibiting plant growth at high concentrations. They must be used in a narrow dosage range to exert their normal effects, which leads to many limitations and inconveniences in practical applications; plant endogenous hormones have natural metabolic pathways Compared with the exogenous synthetic plant growth regulators currently used, the dosage is relatively high, the metabolic pathway is unclear, the chemical properties are stable, and there are many residues or long residual effects. With the continuous expansion of the scope of application, its residue has attracted great attention (Zhao Min et al., Safety of plant growth regulators to crops and the environment, Journal of Environment and Health, 2007 (24) 5: 370-372), my country It has been stipulated that chemically synthesized plant growth regulators shall not be used in the form of fertilizers and pesticides in any green food production and processing (People's Republic of China Agricultural Industry Standard NY-T 393-2000 Guidelines for the Use of Green Food Pesticides; NY-T 394-2000 Green Food Fertilizer Usage Guidelines)

Method used

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  • Application of metal derivative containing chlorophyll and hydrolyzate thereof as plant growth regulator
  • Application of metal derivative containing chlorophyll and hydrolyzate thereof as plant growth regulator
  • Application of metal derivative containing chlorophyll and hydrolyzate thereof as plant growth regulator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Chlorophyllase inhibitory activity (in vitro test) Accurately weigh 1.00 g of acetone powder made from fresh mung bean leaves, extract with 25 mL of phosphate buffer (pH 7.0-7.5) for 4.5 h, centrifuge at 4°C for 10 min, and take the The clear liquid is the chlorophyll enzyme liquid, the active enzyme concentration: 0.14mmol / L, the substrate chlorophyll concentration: 130ppm. Determination of the chlorophyllase inhibition rate of 0.4ppm~25.0ppm each concentration of chlorophyllase metal derivative sample solution (Gan Zhijun, Wang Xiaoyun, Zou Qi. Research on biochemical kinetic characteristics of wheat chlorophyllase. Northwest Botanical Journal, 2003, 23 (5): 750 ~754.), and calculate the IC 50 Value, Series Sample IC 50 The results are shown in the table below

[0032] Chlorophyllase inhibitory activity IC of a series of samples 50 *

[0033]

[0034] * : Substrate concentration 324ppm (0.36mM)

Embodiment 2

[0035] Example 2: Corn seed soaking germination rate and seedling growth time: April 2009; corn variety: Cuitian No. 1; sample: ferric chlorophyllin and ferric chlorophyllin; test method: 0.001ppm~1.0ppm sample solution Corn seeds were soaked for 18 hours, and the water soaking group was the control (CK), and then transferred to soil with the same soil quality and fertility for cultivation, and watered once every 2 days. Compared with the control after 10 days, examine the difference of its germination rate, plant height and main root length (all are average values), the results are shown in the table below

[0036] Comparison of Germination Rate and Seedling Growth of Soaked Corn Seeds

[0037]

[0038]

[0039] The germination rate, plant height and root length of each concentration of the test group were significantly different from those of CK. The test group not only had good growth, but also had rich and well-developed root system, many lateral roots, and main root...

Embodiment 3

[0040] Example 3: Chlorophyllase inhibition experiment in vivo, select mung bean seeds with plump grains and uniform size and soak them in 0.1 ppm solution of the novel plant growth regulator of the present invention for 4 hours, soak the seeds at a room temperature of 28° C. 0.1ppm solution culture in the box, when 1 to 2 pairs of true leaves grow (about 8 days later), use the leaves to make acetone powder, use water culture as a blank (inhibition rate: 0%), and measure the chlorophyllase activity of each treatment group , get the result in the following table

[0041]

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PUM

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Abstract

The invention provides an application of a metal derivative containing chlorophyll and hydrolyzate thereof as a plant growth regulator. The metal derivative containing chlorophyll and hydrolyzate thereof is selected from chlorophyll metal chelate, a chlorophyllin metal chelate and a monomeric metal chelate obtained by separating a chlorophyllin mixture. The chlorophyll is a green pigment extracted from a natural plant raw material and provided with a phaeophytin mother ring structure. The chlorophyllin is a mixture of multiple dihydroporphin components obtained by hydrolyzing the chlorophyll.The monomeric chlorophyllin component is selected from dihydroporphin-e4, dihydroporphin-e6 or phaeophorbide a monomethyl ester. Metal ions comprise bivalent or trivalent transition metal manganese, iron, copper or zinc ions in an oxidation state. The plant growth regulator provided by the invention has the advantages of safety, non-toxicity, low residue, strong activity, good effect, stable product property, controllable and reliable quality and easiness in promotion and application; the chlorophyll content of the plant can be increased, the root growth is promoted, the germination ratio is increased, the stress resistance is enhanced and the yield is also increased.

Description

technical field [0001] The invention relates to a new application of a substance, in particular to the application of a metal derivative containing chlorophyll and its hydrolyzate as a novel plant growth regulator. Background technique [0002] Chlorophyll is the main pigment for photosynthesis in plants, widely exists in the chloroplasts of higher plants, and is the material basis of photosynthesis. In terms of chemical structure, chlorophyll is a kind of compound that chelates metal magnesium. Plant leaves contain about 0.25% of the total chlorophyll, which mainly consists of chlorophyll a, chlorophyll b, chlorophyll c, chlorophyll d and other isomers, which are naturally extracted Chlorophyll is a mixture of these isomers (monomers), and the common point of these monomer isomers is that they have a phorbin structure; chlorophyll has rich chemical reactivity and forms chelates with transition metals It is an important reaction of chlorophyll, and thus a class of products ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01P21/00
Inventor 任勇张列峰蒋慧王一凡童敏金佳敏陈黎明王乐天
Owner NANJING NORMAL UNIVERSITY
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