A plasmonic-photochemical method for grafting carboxyl groups on the surface of cell culture

Abstract

The invention discloses a plasma-photochemical method for grafting carboxyl on a cell culture surface. The method comprises the following steps of: preprocessing the cell culture surface in a plasma reaction chamber, and exposing in air or oxygen for 25 minutes to 24 hours; dissolving a monomer in water to form solution A; spreading the solution A to culture surface obtained by the step (2) uniformly to form a liquid surface layer; irradiating the culture surface on which the liquid surface layer is spread by ultraviolet (UV); and taking the culture surface out of the solution A, and cleaningby the water to obtain the cell culture surface on which the carboxyl is grafted. By the plasma-photochemical method, the carboxyl content of the cell culture surface is improved effectively without using an organic solvent and a light initiator; the damage of the organic solvent and the light initiator to the environment and human bodies in the production process and the potential impact of subsequent stem cell culture are avoided successfully; a step of removing residues is simplified, the production period is shortened, and the cost is saved.

Description

technical field [0001] The invention belongs to the field of material engineering, and in particular relates to a plasma-photochemical method for grafting carboxyl groups on the surface of cell culture. Background technique [0002] With the continuous development of modern science, especially the rapid progress of biomedicine, stem cell culture has become a very important basic work in many research and application fields. For a long time, people have generally used polystyrene culture plates or culture bottles as tools for cell culture. Polystyrene is a colorless and transparent thermoplastic, usually non-toxic, odorless, colorless transparent particles, glass-like brittle material, the general formula is [(CH2CHC6H5)n], the English name is Polystyrene, referred to as PS . Its products have excellent water resistance and moisture resistance. In people's production and life, polystyrene has a wide range of uses, and cell culture is one of the major uses. [0003] The ce...

AI Technical Summary

Problems solved by technology

However, there are some objective deficiencies in these traditional methods: various coating proteins (such as: Laminin) used as surface substrate coating materials are expensive, and it is a severe problem for the limited funds in any related topics. and these proteins are generally animal-derived proteins, which will bring certain risks to human stem cell therapy; the use of serum for stem cell culture has unclear components, complex sources, and differences between batches due to individual differences. This has increased the risk of experimental research and clinical application of stem cell culture; purely using plasma technology to modify the treatment is not ideal under serum-free culture conditions

First of all, the intervention of organic solvents and photoinitiators will inevitably cause residues, which will have certain side effects and risks for the subsequent experimental research and clinical application of stem cell culture

Secondly, the method itself using organic solvents is likely to cause harm to the environment and the human body.

Again, these substances themselves will generate higher costs, coupled with the cost of subsequent removal of residues, will make the application of the results of this technology very expensive

Finally, due to its own characteristics, the existing technology is complicated in practice, time-consuming and laborious