Digital holography-based three-dimensional micro-observation apparatus for cell dynamic of living organism
A technology of digital holography and observation devices, applied in the direction of measuring devices, optical devices, instruments, etc., can solve problems such as limitations, aberrations and distortions, and achieve the effects of convenient operation, good stability, and improved observation resolution
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[0017] The present invention will be further described in detail below in conjunction with the accompanying drawings.
[0018] see figure 1 As shown, the present invention is a dynamic three-dimensional microscopic observation device for biological living cells based on digital holography. Mirror 5, beam bender 6, fiber optic collimating mirror 7, living cell culture dish 8, microscope objective lens 9, relay imaging lens 10, depolarization beam splitter prism 11 and CMOS camera 12; Wherein, the first reflection mirror 3 and the second The structures of the two reflecting mirrors 5 are the same.
[0019] (1) Laser light source 1
[0020] In the present invention, the light source 1 is used to provide laser light 1a of 532nm, i.e. optical information, and the light source provides a single longitudinal mode laser with a center wavelength of 532nm, which can be selected as a Samba TM 532nm laser produced by Cobolt Company in Sweden.
[0021] (2) Optical coupling unit 2
[00...
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