Method for disrupting cell walls of microalgae

Abstract

The invention discloses a method for breaking the cell wall of microalgae. The method mainly includes cell culture, adding microalgae, releasing hydrolytic enzymes, hydrolyzing the cell wall and taking out the original liquid of the microalgae; The bacteria are cultivated in a predetermined space by the culture medium, and microalgae are added in the cultured space according to the number of the bacteria. Adding microalgae is to add the microalgae to be decomposed into the space for cultivating the bacteria, so that the bacteria The body releases hydrolytic enzymes, which can hydrolyze the cell walls of microalgae. After the decomposition of the enzymes, the cell walls can be converted into sugars, and the cell walls of the microalgae will gradually form holes due to decomposition, while the oil in the microalgae can be When the pore diameter of the hole is larger than the volume of the oil, it floats to the culture medium of the bacterial cell culture, and the stock solution remaining in the microalgae can be taken out and classified for use after the cell wall is completely decomposed.

Description

technical field [0001] The invention relates to a method for breaking the cell wall of microalgae, and mainly relates to a biotechnology for microalgae and algae, which is used to facilitate the extraction of the contents of the microalgae and algae. Background technique [0002] With the development of the global economy and the rapid increase of the population, the human demand for oils has also increased. At present, the sources of oils are mainly plant and animal fats, but due to the huge demand, the current plant and animal fats can no longer meet human needs. Therefore, there are gradually existing technologies aimed at obtaining oil from algae. [0003] Because the oil content of most algae is extremely high, take microalgae as an example, microalgae are rich in protein, fat and carbohydrates, and some algae are rich in trace elements and minerals, that is, microalgae have photosynthetic organs such as chlorophyll , can effectively use solar energy to convert H throu...

AI Technical Summary

Problems solved by technology

[0002] With the development of the global economy and the rapid increase of the population, the human demand for oils has also increased. At present, the sources of oils are mainly plant and animal fats, but due to the huge demand, the current plant and animal fats can no longer meet human needs. demand, so there are gradually existing technologies aimed at obtaining oil from algae

[0003] Because the oil content of most algae is extremely high, take microalgae as an example, microalgae are rich in protein, fat and carbohydrates, and some algae are rich in trace elements and minerals, that is, microalgae have photosynthetic organs such as chlorophyll , can effectively use solar energy to convert H through photosynthesis 2 O.CO 2 and inorganic salts into organic compounds, so CO can be absorbed by microalgae 2 To slow down the greenhouse effect, while the reproduction method of microalgae is mainly binary fission, the cell cycle is short, and it is easy to carry out large-scale cultivation, but because the cell wall of microalgae is quite resistant and hard and resistant to degradation, and the cell wall It is composed of cellulose, and cellulose is not conducive to being decomposed by the human body and animals in the body, which in turn reduces the absorption efficiency of the nutrients rich in microalgae, so how to decompose and destroy the cell wall to obtain the original solution in microalgae becomes a technology worthy of research

[0004] As far as we know, the methods of breaking cell walls can be roughly classified into mechanical methods, physical methods, and chemical and biochemical methods. The mechanical methods mainly use mechanical actions to destroy cell walls by grinding or cutting. This method is more suitable for animal tissues. , is not suitable for targeting microorganisms, and is easy to indirectly destroy the original liquid components in cells; physical laws use various physical factors to destroy cell walls, which can be roughly classified into repeated freezing and thawing methods, ultrasonic treatment and other methods. The cells are frozen and then melted at room temperature, and the cell wall structure is broken after repeated several times. This method is suitable for tissue cells, and has a poor effect on microbial cells. The ultrasonic treatment method uses a certain power of ultrasound to make the cell wall violently vibrate and rupture. This method is simple to operate, but it is easy to denature and inactivate some sensitive active substances; the laws of chemistry and biochemistry are divided into methods such as enzymatic dissolution method and chemical infiltration method. The enzymatic dissolution method decomposes the cell wall through various hydrolytic enzymes. It is easy to cause product inhibition, which may affect the release rate of intracellular substances. The cost of lysozyme is low, and the cost of lysozyme is high, so it is not suitable for large-scale use, and different lysozyme must be selected for different strains, which also relatively limits the application of this method. The scope of application, the chemical osmosis method is to use organic solvents to change the permeability of the cell wall so that the contents can seep out. Although this method is relatively mild, due to the long time, the efficiency is relatively reduced. Therefore, regardless of the above or other methods, there are currently Considerable room for improvement or improvement

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