High-throughput cell electrofusion microfluidic chip device based on microwell microelectrode array
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A technology of microelectrode array and microfluidic chip, which is applied in the field of biological cell electrofusion, can solve the problems of inability to accurately realize AB cell pairing and fusion, insufficient electric field strength to achieve fusion, and precise control of unfavorable cells between electrodes. Achieve the effects of improving biocompatibility and anti-oxidation, improving survival ability, and high fusion efficiency
Inactive Publication Date: 2011-12-28
RES COOPERATION FOUND OF YEUNGNAM UNIV
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[0007] However, the traditional cell electrofusion equipment also has some disadvantages: (1) Due to the large electrode spacing in the fusion tank, a high external driving voltage is required to achieve sufficient strength of cell queuing, fusion and compaction signals, often as high as several Hundreds of thousands of volts, high requirements on the electrical safety of the system, the cost of the system is also greatly increased; (2) The large distance between electrodes is not conducive to the precise control of cells, etc.
However, the electrode in this patent is a continuous electrode, and the side wall of the microchannel in the depre
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[0031] The structure and working process of the present invention will be further described below in conjunction with the drawings:
[0032] See figure 1 with figure 2 , This device consists of a microelectrode micropore array chip 1 and a microfluidic cover sheet 2 with top electrode 3.
[0033] Combine image 3 , Figure 4 , Figure 5 with Image 6 It can be seen that the structure of the microelectrode micropore array chip 1 is a quartz substrate layer 4, a microelectrode array layer 5, and a polymer micropore array layer 6 in order from bottom to top.
[0034] Among them, the microelectrode array layer 5 is processed on the quartz base layer 4 using micromachining technology (evaporation, sputtering, and photolithography). The microelectrode array layer 5 can be designed according to the flux required by the experiment. N microelectrode array, the microelectrode 8 is a disk with a diameter of 4-6 microns, the spacing between the microelectrodes 8 is 40 microns, and each micr...
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Abstract
The invention discloses a high-flux cell electrical fusion microfluidic chip device based on a microporous microelectrode array, which consists of a microelectrode microporous array chip and a microfluidic cover plate with top electrodes. In the invention, a micro electro mechanical system (MEMS) processing technique is adopted, and a microporous microelectrode array is constructed on a quartz substrate by using both a microelectrode array and a polymer microporous array; the microporous microelectrode array can better control cell pairing, so that a pair of cells fuses in each micro pore; the array structure can improve the flux of cell fusion; and the materials used for making the device disclosed by the invention have high biocompatibility and corrosion resistance and can ensure the reliability of chip and improve the safety of cell fusion. The device can be widely used in fields of genetics, animal and plant distant hybridization breeding, development biology, medicine screening, preparation of monoclonal antibody, mammalian cloning and the like.
Description
technical field [0001] The invention relates to a device for electrical fusion of biological cells. Specifically, the present invention relates to a chip for providing cell electrofusion, providing and generating the electric field intensity and electric field gradient required for cell alignment, electroporation, and fusion. Background technique [0002] Since the 1980s, cell electrofusion technology has been rapidly developed and widely used because of its advantages such as high efficiency, easy operation, non-toxicity to cells, easy observation, suitable for instrument application and standardized operation (see US patent literature : 4326934, April 27, 1982, Pohl; 441972, April 10, 1982, Pohl; 4578168, March 25, 1986, Hofman; 4695547, Sep 22, 1987, Hillard; 4699881, Oct 13, 1987, Matschke et al. ;). [0003] Cell electrofusion can be divided into two main stages: cell queuing and cell fusion. [0004] The principle of cell queuing is that when biological cells are in...
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