Preparation method and use of enterotoxigenic Escherichia coli egg yolk antibody powder

A technology of Escherichia coli and egg yolk antibodies, applied in the fields of application, anti-bacterial immunoglobulin, immunoglobulin from eggs, etc., can solve the problems of excessive drug residues, endangering human health, and drug resistance, and achieve high accuracy, Good stability and easy storage

Active Publication Date: 2012-01-04
TWINS FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that long-term addition of antibiotics and chemically synthesized drugs in feed will cause excessive drug residues in animal products, pollute the environment, and produce drug resistance, thus endangering human health

Method used

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  • Preparation method and use of enterotoxigenic Escherichia coli egg yolk antibody powder

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: A kind of preparation method of enterotoxic Escherichia coli egg yolk antibody powder comprises the following steps:

[0031] Select 600 healthy commercial laying hens, use porcine ETEC triple vaccine as the antigen to immunize laying hens, intramuscularly inject 1ml / feather to obtain specific IgY, boost immunization once every 15 days, the dose is 1ml / feather, a total of 2 booster immunizations. 50 days after the first immunization, eggs can be collected to prepare E. coli egg yolk antibody powder, and then booster immunization every 40 days to obtain eggs with sustained high titer IgY. The fresh egg yolk slurry is dehydrated and dried within a few seconds by spray drying technology into E. coli egg yolk antibody powder. The spray drying temperature should be controlled at an inlet temperature of 155°C and an outlet temperature of 80°C, and stay in the drying tower for 2 hours. Obtain enterotoxic E. coli egg yolk antibody powder.

Embodiment 2

[0032] Embodiment 2: a kind of preparation method of enterotoxic Escherichia coli egg yolk antibody powder, comprises the following steps:

[0033] Select 600 healthy commercial laying hens, use pig ETEC triple vaccine as the antigen to immunize laying hens, intramuscularly inject 0.25ml / feather, obtain specific IgY, boost immunization once every 18 days, the dose is 0.5ml / feather, a total of 2 booster immunizations Second-rate. 55 days after the first immunization, eggs can be collected to prepare E. coli egg yolk antibody powder, and then booster immunization every 40 days to obtain eggs with sustained high titer IgY. The fresh egg yolk slurry is dehydrated and dried within a few seconds by spray drying technology into E. coli egg yolk antibody powder. The spray drying temperature should be controlled at an inlet temperature of 140°C and an outlet temperature of 78°C, and it should be kept in the drying tower for 2.5 hours. Obtain enterotoxic E. coli egg yolk antibody powde...

Embodiment 3

[0036] Embodiment 3: a kind of preparation method of enterotoxic Escherichia coli egg yolk antibody powder, comprises the following steps:

[0037] Select 600 healthy commercial laying hens, use porcine ETEC triple vaccine as the antigen to immunize laying hens, intramuscularly inject 1ml / feather to obtain specific IgY, boost immunization once every 15 days, the dose is 1ml / feather, a total of 2 booster immunizations. 50 days after the first immunization, eggs can be collected to prepare E. coli egg yolk antibody powder, and then booster immunization every 40 days to obtain eggs with sustained high titer IgY. The fresh egg yolk pulp is dehydrated and dried within a few seconds by spray drying technology into E. coli egg yolk antibody powder. The spray drying temperature should be controlled at an inlet temperature of 135°C and an outlet temperature of 68°C, and it should be kept in the drying tower for 3 hours. Obtain enterotoxic E. coli egg yolk antibody powder.

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Abstract

The invention provides a preparation method and use of safe and environment-friendly enterotoxigenic Escherichia coli (ETEC) egg yolk antibody powder. The preparation method comprises: selecting a healthy commercial egg-laying chicken, immunizing the egg-laying chicken by using pig ETEC triple vaccine as an antigen and injecting through muscle according to a ratio of 0.5 to 2ml/feather, obtaining specific IgY, immunizing for 50 days for the first time, starting to collect eggs, and preparing Escherichia coli egg yolk antibody powder, wherein immunization is performed again every other 40 days for reinforcement, and high-titer IgY eggs can be obtained continuously. Fresh egg yolk pulp is quickly dewatered and dried in several seconds by a spray drying technique to form Escherichia coli egg yolk antibody powder, the spray drying temperature at an inlet is 120 to 155 DEG C, the spray drying temperature at an outlet is 60 to 80 DEG C, and the Escherichia coli egg yolk antibody powder is kept in a drying tower for 2 to 3 hours. The preparation method has the advantages that: the Escherichia coli egg yolk antibody powder can be used as a feed additive for various kinds of livestock and poultry and widely used in livestock and poultry production; and the Escherichia coli egg yolk antibody powder can improve the production performance of piglets in early weanling period, has obvious effects on prevention and treatment of diarrhea in piglets and overcomes the drawbacks of coarse egg yolk antibody.

Description

technical field [0001] The invention relates to an egg yolk antibody, in particular to a preparation method and application of an enterotoxic Escherichia coli egg yolk antibody powder extracted and purified from eggs. Background technique [0002] Egg yolk antibody refers to the antibody against a specific antigen extracted from immunized poultry eggs. When the poultry body is stimulated by foreign antigens, the B cells in the bursa of Fabricius differentiate into plasma cells and secrete specific antibodies into the blood circulation. When the blood flow When passing through the ovary, specific antibodies (mainly IgG) gradually accumulate in the egg cells to form egg yolk antibodies. Due to the role of egg cell receptors, the antibody content in poultry egg yolk is similar to or even higher than that in serum, but it has not attracted enough attention. [0003] In the field of veterinary medicine, IgG has long been isolated from serum, colostrum or monoclonal antibodies of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/12C07K16/02A23K1/16A23K10/20A23K10/22A23K10/28A23K10/30A23K20/142A23K20/158A23K20/26A23K20/28A23K50/30
CPCY02P60/87
Inventor 罗忠华尹荣华黄春玲
Owner TWINS FEED
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