Shewanella-decolorationis-based microbial fuel cell and using method thereof
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A decolorizing Shewanella, fuel cell technology, applied in biochemical fuel cells, sustainable manufacturing/processing, climate sustainability, etc., to achieve rapid metabolism and reproduction, good application prospects, and simplified operation methods
Inactive Publication Date: 2012-01-11
GUANGDONG INST OF MICROORGANISM
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Among them, Geobacter is reported to be able to efficiently convert acetic acid and other substrates into electrical energy, with an efficiency of up to 99%, but Geobacter can only survive under strict anaerobic conditions. Therefore, Geobacter is used in microbial fuel cells. There are certain limitations in research and practical application. In experimental research, strict anaerobic conditions must be ensured by blowing nitrogen and other methods.
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Embodiment 1
[0028] This experiment includes two groups of devices, a control group that maintains an anaerobic environment by blowing nitrogen before starting, and an experimental group that does not blow nitrogen for static reaction.
[0029] The device is built and run as follows:
[0030] Step 1: Build a microbial fuel cell, such as figure 1 As shown: the anode chamber 4 and the cathode chamber 6 are glassy, with a volume of 250ml, separated by a Nafion 112 selective proton permeable membrane 7 .
[0031] Step 2: Put the anode electrode 3 and the cathode electrode 5 into the anode chamber and the cathode chamber respectively, and the electrode material is 2.5×4cm 2 Carbon paper (Toray, Japan), after the device is autoclaved, the anode electrode 3 and the cathode electrode 5 are connected through the external circuit wire 2, and an 800Ω external resistance 1 and a multimeter (UT71D) 8 are connected in series to the external circuit wire. Used to collect data.
[0032] Step 3: Add t...
Embodiment 2
[0036] This experiment included two sets of devices, one with sodium lactate added as an electron donor, and the other without sodium lactate as a control.
[0037] The device is built and run as follows:
[0038] Step 1: Build a microbial fuel cell, such as figure 1 As shown: the anode chamber 4 and the cathode chamber 6 are glassy, with a volume of 250ml, separated by a Nafion 112 selective proton permeable membrane 7 .
[0039] Step 2: Put the anode electrode 3 and the cathode electrode 5 into the anode chamber and the cathode chamber respectively, and the electrode material is 2.5×4cm 2Carbon paper (Toray, Japan), after the device is autoclaved, the anode electrode 3 and the cathode electrode 5 are connected through the external circuit wire 2, and an 800Ω external resistance 1 is connected to the external circuit wire (except when measuring the polarization curve) and Multimeter (UT71D) 8, the multimeter is used to collect data.
[0040] Step 3: Add the reaction solu...
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Abstract
The invention discloses a Shewanella-decolorationis-based microbial fuel cell and a using method thereof. The microbial fuel cell comprises an anode chamber, a cathode chamber, an anode electrode, a cathode electrode, a proton permeation film and an external circuit, wherein a microorganism is inoculated in the anode chamber; the anode chamber is also filled with a culture solution and electron donors of the microorganism; the cathode chamber is filled with cathode reaction liquid; and the microorganism is Shewanella decolorationis. The microbial fuel cell takes the Shewanella decolorationis as an anode microorganism; and the Shewanella decolorationis has extremely high environmental suitability and a variety of breathing mechanisms. In the nutrient-rich anode culture solution, the Shewanella decolorationis can metabolize and reproduce quickly, and consume dissolved oxygen to maintain an anaerobic environment around the anode electrode in an extremely short time; therefore, the step of blowing nitrogen to manufacture the anaerobic environment and keep an anaerobic reaction condition of a common microbial fuel cell before starting or in operation can be eliminated; an operation operating method is simplified; and the Shewanella-decolorationis-based microbial fuel cell has a good application prospect in the treatment and the recycling of environmental pollutants.
Description
Technical field: [0001] The invention relates to a microbial fuel cell and its use method, in particular to a microbial fuel cell based on Shewanella decolorationis and its use method. Background technique: [0002] Microbial fuel cell (MFC) has been rapidly developed and widely used in the research of environmental pollution control and energy conversion in recent years. This technology can directly convert the chemical energy of organic pollutants into electrical energy through the action of microorganisms. MFC has been applied to the degradation research of a variety of difficult-to-treat environmental pollutants, and has shown good application prospects, including a variety of industrial and domestic wastewater, landfill leachate, aromatic compounds, and trichlorethylene. [0003] A general microbial fuel cell includes an anode chamber, a cathode chamber, an anode electrode, a cathode electrode, a proton permeable membrane and an external circuit. Microbes are inoculate...
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