Method for extracting scopoletin from artemisia annua residues

A technology of scopoletin and Artemisia annua, which is applied in the field of extracting scopoletin, can solve the problems of immature and perfect industrial extraction methods, and achieve the effects of saving investment, high purity and simple operation

Inactive Publication Date: 2013-07-03
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been reported that it can be extracted from various plants such as Angelica dahurica, Angelica sinensis, Belladonna, Ding Gong vine, etc., there is no mature and perfect industrial extraction method
Especially the method of extracting scopoletin from the Artemisia annua residue after producing artemisinin has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Add 900kg of Artemisia annua residue into the extraction tank at one time, then add a total of 16000L of acetone (analytical pure) to the extraction tank, add in four times, each addition is 4000L, each time at 53°C and 0.06MP steam After depressing and reflux extraction for 3 hours, collect the extract by filtration, extract 4 times in total, introduce the extract into a concentration tank and concentrate in vacuum at 60°C (the first and second extracts are concentrated separately, the third and fourth extracts Combine and concentrate), recover the acetone solvent; obtain 60kg of crude extract, add 60kg of 80-120 mesh silica gel to mix the sample, and dry it under reduced pressure at 50°C, separate the dried silica gel on a silica gel column by column chromatography, and use petroleum ether (analytical Pure): Ethyl acetate (analytical pure) is eluted with a mixed solvent with a volume ratio of 6:1. During the elution process, different components will be eluted in seque...

Embodiment 2

[0018] Add 900kg of Artemisia annua residue into the extraction tank at one time, then add a total of 16000L of methanol (analytical pure) into the extraction tank, add in four times, each addition is 4000L, each time at 53°C and 0.06MP steam After depressing and refluxing for 2 hours, collect the extract by filtration, and extract 4 times in total. Put the extract into a concentration tank and concentrate in vacuum at 60°C (the first and second extracts are concentrated separately, and the third and fourth extracts Combine and concentrate), recover the methanol solvent; obtain 60kg of crude extract, add 60kg of 80-120 mesh silica gel to mix the sample, and dry it under reduced pressure at 50°C, separate the dried silica gel on a silica gel column by column chromatography, and use petroleum ether (analytical Pure): Ethyl acetate (analytical pure) is eluted with a mixed solvent with a volume ratio of 7:1. During the elution process, different components will be eluted in sequenc...

Embodiment 3

[0020] The same method as in Example 1 was used to extract scopoletin, except that the amount of the used extraction solvent acetone (analytical pure) was different, and the total amount of acetone added was 12000L: during column chromatography, the eluent was petroleum ether (analytical pure) : Ethyl acetate (analytical pure) mixed solvent with a volume ratio of 5:1. The obtained light yellow crude crystals of scopoletin were 1.22 kg.

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Abstract

The invention provides a method for extracting scopoletin. The method comprises the following process steps of: performing heating and refluxing extraction on artemisia annua residues from which artemisinin is extracted and which serves as the raw materials by using methanol, acetone, ethyl acetate or dichloromethane under the conditions of temperature of 53 DEG C and vapor pressure of 0.06 MPa, concentrating; separating by column chromatography; and crystallizing and the like. The artemisia annua residues serve as the raw materials, so the method can be in seamless connection with the production line of industrialized extraction of the artemisinin, investment of purchasing the raw materials is saved and the cost of the raw materials is greatly reduced. The method is easy to operate. By the method, the scopoletin can be effectively extracted; and the purity of the scopoletin in the obtained extracts is high.

Description

technical field [0001] The invention relates to the technical field of natural product separation engineering, in particular to a method for extracting scopoletin from Artemisia annua residues. Background technique [0002] Scopoletin is a coumarin compound that exists widely in nature. It has been reported that it can be extracted from various plants, such as Angelica dahurica, Angelica sinensis, Belladonna, Dinggongteng and so on. Scopoletin can regulate blood pressure and has antibacterial activity against different types of bacteria, including Escherichia coli, Staphylococcus aureus, Streptococcus, Klebsiella pneumoniae and Pseudomonas aeruginosa. Scopoletin has anti-inflammatory activity and is useful in the treatment of bronchial diseases and asthma. It also regulates the hormone serotonin, which helps reduce anxiety and depression. The applicant also found that scopoletin has obvious acaricidal activity against important agricultural pest mites such as Tetranychus c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/16
Inventor 丁伟秦培元张永强杨振国罗金香李阳
Owner SOUTHWEST UNIV
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