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Preparation method of phyllemblic tannin

A technology of emblica and tannin, which is applied to chemical instruments and methods, medical preparations containing active ingredients, and pharmaceutical formulas, etc., can solve the problems of less research on tannin, achieve low cost, improve extraction efficiency, and good separation effect Effect

Inactive Publication Date: 2012-01-25
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, domestic research on tannins is less and still in its infancy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Grind the dried fruit of Amla emblica, take 1kg, put it into a supercritical extraction device, extract at a temperature of 35°C and a pressure of 20MPa for 100min, add 8L of 80% ethanol solution to the residue for ultrasonic extraction, and extract 3 times at an ultrasonic frequency of 25KHz , 1 hour each time, filter, combine the extracts, concentrate, add a 3% gelatin solution, stir until no precipitation occurs, centrifuge, dissolve the precipitate with 50% acetone aqueous solution, filter the gelatin to obtain a preliminary purified solution, and put on 1L HPD -400 macroporous resin column, after the adsorption is completed, first elute with 4L of water, then elute with 4L of 70% ethanol solution, control the flow rate to 3ml / min, collect the eluate rich in emblical tannin, concentrate, freeze-dry That is, 158 g of emblical tannin was obtained.

[0020]

Embodiment 2

[0022] Grind the dried fruit of Amla emblica, take 1kg, put it into a supercritical extraction device, extract at a temperature of 45°C and a pressure of 30MPa for 50min, add 5L of 70% ethanol solution to the residue for ultrasonic extraction, and extract twice at an ultrasonic frequency of 35KHz , 1 hour each time, filter, combine the extracts, concentrate, add a 6% gelatin solution, stir until no precipitation occurs, centrifuge, dissolve the precipitate with 90% acetone aqueous solution, filter the gelatin to obtain a preliminary purified solution, and put it on 1L HPD -400 macroporous resin column, after the adsorption is completed, first elute with 5L of water, then with 5L of 70% ethanol solution, control the flow rate at 4.5ml / min, collect the eluate rich in emblical tannins, concentrate, and freeze After drying, 207g of emblical tannin was obtained.

[0023]

Embodiment 3

[0025] Grind the dried fruit of Amla emblica, take 1kg, put it into a supercritical extraction device, extract at a temperature of 40°C and a pressure of 30MPa for 60min, add 10L of 70% ethanol solution to the residue for ultrasonic extraction, and extract twice at an ultrasonic frequency of 30KHz , 2 hours each time, filter, combine the extracts, concentrate, add a 5% gelatin solution, stir until no precipitation occurs, centrifuge, dissolve the precipitate with 70% acetone aqueous solution, filter the gelatin to obtain a preliminary purified solution, and put it on 1 LADS -17 macroporous resin column, after the adsorption is completed, first elute with 3L of water, then with 6L of 70% ethanol solution, control the flow rate at 3.5ml / min, collect the eluate rich in emblical tannins, concentrate, and freeze After drying, 122 g of emblical tannin was obtained.

[0026]

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PUM

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Abstract

The invention discloses a preparation method of phyllemblic tannin. In the preparation method, phyllemblic dried fruits are used as raw materials. The process flow mainly comprises three parts: firstly, extracting grease substances in a phyllemblic medicinal material with supercritical carbon dioxide fluid and carrying out ultrasound extraction to obtain an extraction solution; secondly, condensing the extraction solution, adding a gelatine solution and centrifuging, dissolving a precipitate with an acetone water solution and filtering gelatine to obtain a preliminary purified solution; and thirdly, feeding the preliminary purified solution to macroporous resin for purifying, eluting with water and an ethanol water solution, condensing an eluent, freezing and drying to obtain the phyllemblic tannin. The preparation method disclosed by the invention is carried out below 50 DEG C and the change of chemistry and structure of tannin components in the conventional heat reflow extraction process caused by over-high temperature is avoided, so that the tannin with higher purity can be obtained by using the method and the tannin content in the product is greater than 55 percent. In addition, the method has a short extraction period, is safe and is suitable for industrial production. The invention lays a foundation for deeply researching pharmacological activities of the phyllemblic tannin components and a medicinal preparation.

Description

technical field [0001] The invention relates to a method for preparing active ingredients of traditional Chinese medicine, in particular to a method for preparing tannin from emblica fruit. Background technique [0002] Phyllanthus emblica is the fruit of euphorbiaceae plant oil orange and emblica, which has the effects of reducing phlegm, promoting body fluid, relieving cough, and detoxifying. Distributed in Fujian, Guangdong, Guangxi, Yunnan, Guizhou, Sichuan, Taiwan and other places. Most of emblica are wild or semi-wild, almost free from industrial pollution, and are veritable raw materials for green food processing. [0003] The emblical fruit contains 45% tannin, and the immature fruit contains 30%-35% tannin, of which chebrinic acid, chebulagic acid, corilagin, Orthochebulic acid (terchebin), chebulacic acid (split acid) and gallic acid, ellagic acid. It also contains phyllemblic acid, emblicol, mucie acid, and rich vitamin C (1.0-1.8g / 100g). [0004] At present, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/47C07H13/08A61P1/02A61P11/04A61P11/14A61P29/00A61P31/04A61K131/00
CPCY02P20/54
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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