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Biomacromolecular hydrogel biological stent and preparation method thereof

A bio-macromolecule and bio-scaffold technology is applied in the field of alginate hydrogel bio-scaffold and its preparation, which can solve the problems of residual, residual and high cost of bio-scaffolds

Active Publication Date: 2012-02-01
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] 2. The process is complicated. The preparation of biological scaffolds by the pressure drop / particle precipitation method requires the preparation of a polymer solution with an appropriate viscosity, the preparation of a pore-forming agent of a certain size, and the need to adjust the air pressure and vacuum pump to obtain an appropriate pressure drop and other tedious steps.
[0011] 3. Organic reagent residues, the polymer solution contains toxic solvent dimethylformamide, even if there is a solvent evaporation step in the preparation process, but there will still be residues in the biological scaffold; Residues in the bioscaffold during the process
[0012] Aiming at the problems of residual organic reagents, complex process and high cost in the preparation of biological scaffolds, the purpose of the present invention is to use layer-by-layer assembly technology to prepare alginate hydrogel biological scaffolds cross-linked by cross-linking agents in aqueous solution. In situ generation of CO from water-soluble carbonates 2 The method of foaming makes holes in the bioscaffold, providing a simple, practical, controllable, clean and low-cost method to prepare bioscaffolds

Method used

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  • Biomacromolecular hydrogel biological stent and preparation method thereof
  • Biomacromolecular hydrogel biological stent and preparation method thereof
  • Biomacromolecular hydrogel biological stent and preparation method thereof

Examples

Experimental program
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Effect test

preparation example 1

[0056] For the preparation process of alginate hydrogel bioscaffold, see figure 2 , the prepared tubular hydrogel bioscaffold see image 3 .

[0057] First cut the polycarbonate tube (a), seal both ends of the tube with polyethylene parafilm, and then immerse the polycarbonate tube in 30mg / mL sodium alginate aqueous solution for 5min (b), the surface of the polycarbonate tube adsorbs sodium alginate Then take out and immerse in 4mol / L GaGl 2 10min in aqueous solution to make it completely cross-linked (c), and then placed in pure water for 10min to wash excess Ca 2+ and Cl - (d). Repeat the above steps as needed to prepare multilayer hydrogel (e), and separate the multilayer hydrogel layer by layer to obtain tubular hydrogel bioscaffolds (f) with different diameters. To prepare porous hydrogel bioscaffolds, add CaCO to aqueous sodium alginate 3 Granules, mixed well, prepared as above steps containing CaCO 3 The granular tubular hydrogel bioscaffold was soaked in 1mol / L...

preparation example 2

[0062] First cut the polyethylene tube, seal both ends of the tube with polyethylene parafilm, immerse the polyethylene tube in 20mg / mL potassium alginate aqueous solution for 10min, take it out and immerse it in 3mol / L BaCl2 aqueous solution for 20min to make it completely cross-linked, then Soak in pure water for 20min to clean excess Ba 2+ and Cl - , and finally immersed in 10mg / mL chitosan aqueous solution for 20min to adsorb chitosan through electrostatic action. By repeating the above steps, a hydrogel bioscaffold containing two biomacromolecules, alginate and chitosan, can be prepared. To prepare porous hydrogel bioscaffolds, add BaCO to aqueous potassium alginate 3 Granules, mixed evenly, prepared as above steps containing BaCO 3 The granular tubular hydrogel bioscaffold was soaked in 2mol / L HCl aqueous solution to the BaCO in the scaffold 3 The particles completely dissolve to form CO 2 The porous hydrogel bioscaffold was prepared after washing with pure water. ...

preparation example 3

[0064] First cut the polycarbonate tube, seal both ends of the tube with polyethylene parafilm, and immerse the polycarbonate tube in a solution containing MgCO 3 The particles were soaked in 10mg / mL polyvinyl alcohol aqueous solution for 20min, then taken out and immersed in 3wt.% glutaraldehyde aqueous solution for 30min to make them completely cross-linked, and then placed in pure water for 60min to clean excess glutaraldehyde. Repeat the above steps as needed, will contain MgCO 3 Granular polyvinyl alcohol hydrogel bioscaffold soaked in 2mol / L HCl aqueous solution to MgCO in the scaffold 3 The particles completely dissolve to form CO 2 The porous polyvinyl alcohol hydrogel bioscaffold was prepared after washing with pure water. Evaporate the porous hydrogel bio-scaffold in a 90° C. dry oven and then immerse it in 3 wt.% gelatin aqueous solution to swell, so that the gelatin active component can be modified on the surface of the hydrogel bio-scaffold.

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Abstract

The invention discloses a biomacromolecular hydrogel biological stent and a preparation method thereof and more specifically relates to an alginate hydrogel biological stent and a preparation method thereof. In the invention, the alginate hydrogel biological stent cross-linked by using a cross-linking agent is prepared in a water system by applying a layer-by-layer assembly technology, and pores are formed in the biological stent by using a method of generating CO2 foams in situ by using water-insoluble carbonate. The invention provides the simple and practical method, which is strong in controllability and low in cleaning cost, for preparing the biological stent.

Description

technical field [0001] The present application relates to a biomacromolecule hydrogel bioscaffold and a preparation method thereof, more specifically, to an alginate hydrogel bioscaffold and a preparation method thereof. Background technique [0002] The preparation of bioscaffolds is one of the important contents of tissue engineering research. An ideal bioscaffold should have the following characteristics: 1. It has good biocompatibility, can promote cell adhesion and provide suitable microstructure for cell proliferation on its surface. Environment, no cytotoxicity when cultured in vitro, and will not cause inflammation and rejection of the body when implanted in the body; 2. It has a three-dimensional structure, and the interior is interconnected to form an open channel structure, which provides enough space for cells to adhere and is conducive to nutrients. and the transmission of metabolites; 3. It is biodegradable, matching the proliferation rate of cells, and the deg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/16A61L27/52A61L27/54A61L27/56A61L27/50A61F2/00A61F2/06A61F2/18A61F2/28C08L5/04C08L29/04C08L33/02C08L5/08C08J3/24C08J3/075A61F2/82
Inventor 李峻柏杜明春崔岳
Owner INST OF CHEM CHINESE ACAD OF SCI
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