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Alkaline Bacilluspseudofirmus MC02 and application thereof

A technology of Bacillus firmus and strains, applied in the field of environmental biology, can solve the problems of undiscovered Bacillus firmus and unfavorable microbial growth, etc., and achieve the effect of strong humic acid reducing ability, wide application prospect, and wide electron utilization spectrum

Active Publication Date: 2012-02-01
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, alkaline conditions are relatively unfavorable to the growth of microorganisms. Under such harsh conditions, microorganisms may domesticate a set of mechanisms to better cope with harsh environments
At present, no reports of Bacillus firmus-like bacteria with Fe(III) reduction and humic acid reduction activities have been found.

Method used

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  • Alkaline Bacilluspseudofirmus MC02 and application thereof
  • Alkaline Bacilluspseudofirmus MC02 and application thereof
  • Alkaline Bacilluspseudofirmus MC02 and application thereof

Examples

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Embodiment Construction

[0013] Enrichment and isolation of strain MC02

[0014] 1) Tear off the anode carbon felt from the sludge microbial fuel cell with a pH of 9.0 and soak it in 50mL liquid enrichment medium (the configuration of the sludge microbial fuel cell is as described in CN200910041235.4), liquid enrichment and separation culture The composition of the base is: 16g AQDS (electron acceptor), 0.25g NH 4 Cl, 0.678g NaH 2 PO 4 2H 2 O, 0.1g KCl, 2.94g NaHCO 3 , 1.59g Na 2 CO 3 , 10.0mL vitamin solution, 10.0mL trace element solution (pH 9.0). Among them, the vitamin solution contains 2.0mg biotin, 2.0mg folic acid, VB6 10.0mg vitamin, 5.0mg thiamine, 5.0mg riboflavin, 5.0mg niacin, 5.0mg calcium pantothenate, 0.1mg vitamin per liter of deionized water B12, 5.0mg p-aminobenzoic acid, 5.0mg lipoic acid; the trace element solution contains 1.5g nitrilotriacetic acid, 3.0g MgSO per liter of deionized water 4 ·7H 2 O, 0.5g MnSO 4 ·H 2 O, 1.0g NaCl, 0.1g FeSO 4 ·7H 2 O, 0.1g CoCl ...

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Abstract

The invention discloses alkaline Bacilluspseudofirmus MC02 CGMCC (China General Microbiological Culture Collection Center): 4772 and application of the alkaline Bacilluspseudofirmus MC02 in reduction of iron oxides and humus. The Bacilluspseudofirmus MC02 is obtained by separating and purifying an alkaline sludge microbial fuel cell anode biofilm which well operates; and under the alkaline condition, the Bacilluspseudofirmus MC02 has strong Fe(III) and AQDS (anthraquinone-2,6-disulfonic acid) reducing capacity, broad electron utilization spectrum and wide application prospect.

Description

technical field [0001] The invention belongs to the field of environmental biotechnology, and in particular relates to a strain of Bacillus firmus ( Bacillus pseudofirmus ), and its application in humus reduction and iron reduction under alkaline anaerobic conditions. Background technique [0002] Humic respiration is a form of microbial respiration metabolism that is ubiquitous in anaerobic environments. Humus respiration, also known as quinone respiration, refers to the process in which microorganisms use humus as the only terminal electron acceptor to accept electrons provided by organic acids and other substances, couple energy generation, and support the growth of bacteria. Humus respiration is closely related to iron reduction. Humus respiration is of great significance in the biogeochemical cycle of elements, as follows: 1) During the process of humus respiration, microorganisms can directly oxidize organic matter or toluene, vinyl chloride and other environmental t...

Claims

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Application Information

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IPC IPC(8): C12N1/20C21B15/00A62D3/02C12R1/07A62D101/43
Inventor 马晨周顺桂张隽杨贵芹
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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