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Method for preparing succinic acid from bagasse by fermentation

A technology for succinic acid and production of succinic acid, which is applied in the field of bioengineering, can solve the problem of high raw material cost of succinic acid, and achieve the effect of relieving the national energy crisis, good effect, simplified process procedures and cost

Inactive Publication Date: 2014-07-02
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] The technical purpose of the present invention is to provide a new method of fermenting and preparing succinic acid using bagasse as a carbon source or even replacing part of the nitrogen source, so that the preparation of succinic acid by fermentation of this method can further reduce the preparation cost of bagasse fiber hydrolyzed sugar solution , can also use bagasse to replace part of the expensive nitrogen source, solve the technical problem of high raw material cost of succinic acid produced by traditional fermentation, and realize the advantages of efficient use of renewable resources and environmental friendliness, showing its bright industrial application prospects

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  • Method for preparing succinic acid from bagasse by fermentation
  • Method for preparing succinic acid from bagasse by fermentation
  • Method for preparing succinic acid from bagasse by fermentation

Examples

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Embodiment 1

[0033] This example illustrates the steps of producing succinic acid by fermenting and culturing bagasse to produce succinic acid according to the present invention.

[0034] (1) Ultrasonic treatment of bagasse to make the sugar in it more favorable for hydrolysis: mix bagasse and dilute sulfuric acid at a mass volume ratio (w / v) of 15-20%, ultrasonic time is 20-60 min, and ultrasonic temperature is 15 ~25°C, ultrasonic frequency is 80-150 Hz.

[0035] Further, the volume content of the dilute sulfuric acid is 1%-2% (v / v).

[0036] (2) Prepare multi-component sugar solution by dilute acid hydrolysis method: heat the material solution obtained in step (1) to a temperature of 120-130°C, hydrolyze for 150-180 minutes, and obtain a hydrolyzate by suction filtration, adjust the pH to 6.0, 115 After being sterilized at ℃ for 15 minutes, it was used as a carbon source for later use.

[0037] (3) The spare carbon source is directly added to the fermentation medium without carbon sou...

Embodiment 2

[0044] The succinic acid-producing microbial strains used in this example are: Actinobacillus succinate NJ113 ( Actinobacillus succinogenes NJ113), the bacterium has applied for a patent and obtained authorization, and the patent authorization announcement number is CN100537744C.

[0045] Firstly, succinic acid was produced by fermenting the fiber hydrolyzed sugar liquid obtained from bagasse raw material without ultrasonic treatment as carbon source with different total sugar concentrations, in order to find out the optimum total sugar concentration suitable for the preparation of succinic acid from bagasse fiber sugar liquid. The results are shown in Table 1. The optimum total sugar concentration was 30 g / L, and 19.7 g / L succinic acid could be produced. The fermentation culture conditions described are: anaerobic, fermentation temperature 30°C, fermentation time 72 h; inoculum size: 10%.

[0046]

[0047] Example 2

[0048] The succinic acid-producing microbial stra...

Embodiment 3

[0054] The succinic acid-producing microbial bacterial strain that the embodiment adopts is: Actinobacillus succinogenes NJ113 ( Actinobacillus succinogenes NJ113), the bacterium has applied for a patent and obtained authorization, and the patent authorization announcement number is CN100537744C. Firstly, succinic acid was prepared by fermenting the bagasse hydrolyzed sugar solution without detoxification treatment with a total initial sugar concentration of 30 g / L as the carbon source. The results are shown in Table 4, and the yield of succinic acid was 23.2 g / L.

[0055] The traditional detoxification method refers to: use 1% to 3% activated carbon (w / v) and multi-component sugar solution prepared by dilute acid hydrolysis as reactants, mix well, pH range 4-6, place in In a water bath, stir at a constant speed at 40-60°C, and the reaction can be completed in 30 minutes. The fermentation culture conditions described are: anaerobic, fermentation temperature 37°C, fermentatio...

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Abstract

The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing succinic acid by fermenting bagasse. The method of the present invention includes the steps of succinic acid producing bacteria strain activation, seed cultivation and fermentation culture to produce succinic acid, and its technical gist is that the steps of fermenting and cultivating succinic acid production include ultrasonic treatment of bagasse, hydrolysis with dilute acid The method involves the steps of preparing multi-component sugar solution, adding spare carbon source directly to the fermentation medium without carbon source, saving the amount of nitrogen source, and inoculating to produce acid. Using this method to ferment and prepare succinic acid can further reduce the preparation cost of bagasse fiber hydrolyzed sugar liquid, and can also replace part of the expensive nitrogen source with bagasse, which solves the technical problem of high raw material cost of traditional fermentation to produce succinic acid, and realizes high efficiency. The use of renewable resources, environmental friendliness and other advantages show its bright industrial application prospects.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing succinic acid by fermenting bagasse. Background technique [0002] Succinic acid, commonly known as succinic acid, as a common natural organic acid, widely exists in animals, plants and microorganisms. Many anaerobic microorganisms use succinic acid as the main end product of their energy metabolism. As an excellent C4 platform compound, succinic acid can be widely used in medicine, fine chemical products and biodegradable polymer precursors, such as 1,4-butanediol, tetrahydrofuran, fatty acid, γ-butane Intermediates such as lactones, and these chemicals have great market potential. [0003] Under the threat of food shortage, exploring the production of succinic acid from lignocellulosic raw materials has become an important component of the development strategy of chemical raw materials, and it is also a research hotspot. Lignocellulos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/46C12R1/01C12R1/19C12R1/15
Inventor 姜岷奚永兰戴文宇张九花徐蓉陈可泉韦萍
Owner NANJING TECH UNIV
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