Method for rapid determination of oil content in Camellia oleifera seeds by using near-infrared diffuse reflectance spectroscopy (NIRS)

A rapid determination of Camellia oleifera seed technology, applied in the field of edible vegetable oil quality analysis, to achieve the effect of good application value

Inactive Publication Date: 2012-02-15
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
View PDF4 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, domestic and foreign studies on NIRS analysis of the o

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapid determination of oil content in Camellia oleifera seeds by using near-infrared diffuse reflectance spectroscopy (NIRS)
  • Method for rapid determination of oil content in Camellia oleifera seeds by using near-infrared diffuse reflectance spectroscopy (NIRS)
  • Method for rapid determination of oil content in Camellia oleifera seeds by using near-infrared diffuse reflectance spectroscopy (NIRS)

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0039] Example 1

[0040] A near-infrared diffuse reflectance spectroscopy (NIRS) method for quickly determining the oil content of camellia seeds, which is characterized in that the near-infrared diffuse reflectance spectroscopy analysis technique measures the oil content in different varieties of camellia seeds, and consists of the following steps:

[0041] The first step is the selection of high-quality camellia varieties

[0042] Select high-quality varieties from 120-150 Camellia seed varieties, with oleic acid content of 65%-85%, preferably 70%-80%, linoleic acid content of 5%-20%, preferably 7%-12%, palmitic acid content 3%-15%, preferably 7%-11%, stearic acid content 1%-5%, preferably 1%-3%;

[0043] The second step is to determine the basic data of camellia seed samples

[0044] The Soxhlet extraction method was used to determine the oil content of camellia seed oil, and the content of fatty acids in the camellia oil was analyzed by gas chromatography.

[0045] The third step i...

Example Embodiment

[0054] Example 2 Determination of oil content of different varieties of camellia seeds

[0055] Crush the dried camellia seeds into powder (containing the seed shell, the sample moisture content is less than 10%), weigh a certain amount of camellia seed powder, and record the weight m 0 . Put it into the filter paper tube, stuff absorbent cotton into the upper part, compress the sample, and put it into the extractor. Add 180 mL of petroleum ether (60℃-90℃) into the extraction bottle, and extract at 80℃ for 6 hours. After the extract is evaporated and the solvent is concentrated, the tea oil is obtained. Weigh the oil in m 1 . Calculate the oil content of the corresponding variety w=m 1 / m 0 . The oil content of 130 varieties of camellia seeds is shown in Table 3 (samples No. 1 to No. 120 are from Jiangxi).

[0056] In this example, different varieties of camellia seed samples from Yunnan, Jiangxi, Zhejiang and other places were selected. There are 26 varieties with oil content o...

Example Embodiment

[0057] Example 3 Determination of fatty acid composition in different varieties of camellia oil by gas chromatography

[0058] The selected varieties in this example are Guangxi (Lingyun, Liuzhou Rongshui, Fangchengdongzhong, Baise, Fengshan, Rong'an, Tianyang, Nandan, Ordinary Camellia), white camellia oil, safflower tea oil, Yunnan Funingzhan 129 samples (as shown in Table 3) including 1 to 120 samples from North Korea and Jiangxi were dropped in a small amount into a 10mL test tube, and 2mL 0.5mol / L NaOH-CH was added 3 OH, shake well, put it in a 60°C water bath for methyl esterification for 30 minutes, take it out and add 5 mL of n-hexane, shake it well, take out the supernatant after standing, and perform GC chromatographic analysis.

[0059] GC analysis conditions: SE-30 is selected for the chromatographic column; flame ionization detector (FID) for hydrogen; inlet temperature is 280°C, detector temperature is 280°C, column temperature is 200°C, lasts 5 minutes, and heats up a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for rapid determination of oil content in Camellia oleifera seeds by using NIRS. According to the invention, a high-quality Camellia oleifera seed variety is selected from over 130 Camellia oleifera seed varieties, and the selected Camellia oleifera seed variety contains 70% to 80% of tea oil oleic acid, 7% to 12% of linoleic acid, 7% to 11% of palmitic acid and 1% to 3% of stearic acid; chemical values of oil content in selected Camellia oleifera seeds are analyzed, and a precise analysis model is established by combining the chemical values with NIRS; combined pretreatment methods of first derivative, SG (Savitzky-Golay filter) and MSC (multiplicative signal correction) produce a best effect, optimal wave bands are 4200.20 to 4088.35/cm and 4666.89-4639.89.89/cm, and an optimal regression method is the method of partial least squares; oil content in Camellia oleifera seed samples to be determined can be obtained by analyzing near-infrared spectrogram of the Camellia oleifera seed samples to be determined by using an established correction model, and a correlation coefficient between analytical values and predicted values can reach 0.8978. The method provided in the invention can be used for rapid and accurate determination of oil content in unknown Camellia oleifera seeds and has a good application value in high-quality breeding and quality analysis of Camellia oleifera.

Description

technical field [0001] The invention relates to the field of edible vegetable oil quality analysis, in particular to a method for quickly measuring the oil content of camellia oleifera seeds by near-infrared diffuse reflectance spectroscopy (NIRS). Background technique [0002] Camellia oleifera (Camellia oleifera), an evergreen small tree in the family Theaceae, is a unique resource in my country. It is as famous as oil palm, olive and coconut as the world's four largest woody edible oil source tree species. Camellia oleifera is mainly distributed in more than ten provinces such as Hunan, Jiangxi, Guangxi, and Zhejiang in my country. The unsaturated fatty acid content in camellia oil is as high as 93%, of which oleic acid is 74% to 87%, linoleic acid is 7% to 14%, and its monounsaturated fatty acid content is the highest among edible oils at present. In addition, tea oil is also rich in vitamin E, vitamin D, carotene, phospholipids, etc. Long-term consumption of tea oil is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/35G01N21/3563G01N21/359
Inventor 王成章原姣姣陈虹霞周昊叶建中
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap