Method for producing glycyrrhetinic acid through microbial fermentation transformation
A technology of microbial fermentation and glycyrrhetinic acid, which is applied in the field of microorganisms, can solve the problems of small microbial transformation of glycyrrhetinic acid and has not yet formed a large-scale level, so as to improve the utilization rate of raw materials, reduce waste liquid discharge and environmental pollution, and reduce industrial pollution. The effect of production costs
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Embodiment 1
[0040] Inoculate Aspergillus flavus (Aspergillus flavus) CGMCC No 5144 into the Erlenmeyer flask containing the seed medium, the pH is natural, the inoculum size is 1%, the rotation speed is 200rpm, and the ventilation volume is 0.2m 3 / h, and the incubation time was 24 hours.
[0041] Transfer the above cultured strains into a 100L fermenter (with 60L of sterilized fermentation medium inside) according to the inoculum amount of 30%, adjust the fermentation temperature to 10°C, and the ventilation rate to 0.4m 3 / h, stirring speed 150rmp, fermentation 3d.
[0042] Investigate the production of glycyrrhetinic acid. Detection of glycyrrhetinic acid by vanillin sulfate method:
[0043] (1) Reference substance solution preparation
[0044] Accurately weigh 20 mg of glycyrrhetinic acid standard product dried at 100°C to constant weight, put it in a 20 ml measuring bottle, add absolute ethanol to the mark, and obtain a glycyrrhetinic acid standard solution with a content of 1 mg / ...
Embodiment 2
[0050] Inoculate Aspergillus flavus (Aspergillus flavus) CGMCC No 5144 into the Erlenmeyer flask containing the seed medium, the pH is natural, the inoculum size is 1%, the rotation speed is 200rpm, and the ventilation volume is 0.2m 3 / h, and the incubation time was 24 hours.
[0051] Transfer the above cultured strains into a 100L fermenter (with 60L of sterilized fermentation medium inside) according to the inoculum amount of 30%, adjust the fermentation temperature to 20°C, and the ventilation volume to 0.4m 3 / h, stirring speed 150rmp, fermentation 3d, strain fermentation concentration reached 3.1g / L.
[0052] Glycyrrhetinic acid detection method is the same as above.
Embodiment 3
[0054] Inoculate Aspergillus flavus (Aspergillus flavus) CGMCC No 5144 into the Erlenmeyer flask containing the seed medium, the pH is natural, the inoculum size is 1%, the rotation speed is 200rpm, and the ventilation volume is 0.2m 3 / h, and the incubation time was 24 hours.
[0055] Transfer the above cultured strains into a 100L fermenter (with 60L sterilized fermentation medium inside) according to the inoculum amount of 30%, adjust the fermentation temperature to 30°C, and the ventilation volume to 0.4m 3 / h, stirring speed 150rmp, fermentation 3d, bacterial strain fermentation concentration reaches 4.2g / L.
[0056] Glycyrrhetinic acid detection method is the same as above.
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