Method for producing glycyrrhetinic acid through microbial fermentation transformation

A technology of microbial fermentation and glycyrrhetinic acid, which is applied in the field of microorganisms, can solve the problems of small microbial transformation of glycyrrhetinic acid and has not yet formed a large-scale level, so as to improve the utilization rate of raw materials, reduce waste liquid discharge and environmental pollution, and reduce industrial pollution. The effect of production costs
CN102363796AActive Publication Date: 2012-02-29中科医药行业生产力促进中心有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
中科医药行业生产力促进中心有限公司
Publication Date
2012-02-29

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Abstract

The invention provides a method for producing glycyrrhetinic acid through microbial fermentation transformation. The method comprises the following steps of: inoculating aspergillus flavus CGMCC No 5144 into a solid potato dextrose agar (PDA) slant culture medium to cultivate; inoculating 1 percent of the cultivated aspergillus flavus CGMCC No 5144 to a culture medium containing fresh seeds from the inclined surface to cultivate; inoculating 30 percent of the strains obtained from the seed culture medium to a fermentation tank containing a fresh fermentation culture medium and performing fermentation culture to obtain fermentation liquid containing the glycyrrhetinic acid; separating a glycyrrhetinic acid coarse product from the fermentation liquid by a membrane separation combined process; and preparing high-purity glycyrrhetinic acid by a purification process. In the glycyrrhetinic acid finally obtained by the method, the fermentation quantity reaches 4.38 g / L, the product purity is more than or equal to 98 percent, the conversion rate is more than or equal to 90 percent, and the technological scale level is reached.
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Description

technical field

[0001] The invention relates to the technical field of microorganisms, in particular to a technology for transforming and producing glycyrrhetinic acid with microorganisms. Background technique

[0002] Licorice is a traditional Chinese medicinal material in my country, known as "the old man of the country", sweet in taste and flat in nature. Guixin, lung, spleen, stomach. Invigorating the spleen and replenishing qi, clearing away heat and detoxification, eliminating phlegm and relieving cough, relieving spasm and relieving pain, harmonizing various medicines. For weakness of the spleen and stomach, lassitude and weakness, palpitation and shortness of breath, cough with copious phlegm, epigastric abdomen, extremity convulsion acute pain, carbuncle sore tumefacting virus. It can alleviate the toxicity and potency of drugs. Licorice is widely used in medicine, tobacco, food and other fields. Glycyrrhizic acid and glycyrrhetinic acid are the main active ingr...

Claims

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