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Method for preparing lucid ganoderma mycelium liquid from slag water generated in production of starch

A technology of Ganoderma lucidum mycelium and starch, which is applied in the directions of botanical equipment and methods, application, fertilizer mixture, etc., can solve the problems of many raw material components of liquid fermentation medium, can not save energy and manpower, complex production process, etc., and achieves reduction of raw materials. The process of preparation and high temperature steam sterilization, the effect of saving energy and manpower, and the value of a wide range of applications

Inactive Publication Date: 2012-03-14
甘肃盛亚肽生物科技有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The traditional method of preparing mycelium liquid by liquid fermentation of Ganoderma lucidum uses a liquid medium prepared from glucose, peptone, yeast extract, soybean flour, corn flour, vitamins, inorganic salts and distilled water. The shredded pieces are inoculated in the liquid medium, and the ganoderma lucidum mycelium liquid is prepared by aerobic fermentation. The disadvantages are that the liquid fermentation medium used has many raw materials, high cost, complicated production process, and can not save energy and manpower.

Method used

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  • Method for preparing lucid ganoderma mycelium liquid from slag water generated in production of starch
  • Method for preparing lucid ganoderma mycelium liquid from slag water generated in production of starch
  • Method for preparing lucid ganoderma mycelium liquid from slag water generated in production of starch

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1: see figure 1 , the method for preparing ganoderma lucidum mycelium fluid with the slag water produced by producing potato starch as a medium, its main feature is that the preparation steps are:

[0028] (1) Preparation of Ganoderma lucidum slant strains: Inoculate the ganoderma lucidum mycelium block in the mother seed test tube slant surface on the slag water agar slant medium produced by potato starch production, and activate it by static culture at 24-26°C for 4-6 days. Then replant it on the slack-water sawdust agar slant medium produced by the production of potato starch, and carry out the second activation by static culture at 24-26°C for 4-6 days, and it is a first-class strain;

[0029] The slag water agar slant medium produced by the production of potato starch: add 1.8 g of glucose and 1.8 g of agar to 80 ml of slag water produced by the production of potato starch, and the pH value is natural. Minutes of high-temperature steam sterilization, and t...

Embodiment 2

[0044] Example 2: see figure 1 , the method for preparing ganoderma lucidum mycelia liquid with the slag water that produces potato starch as medium, its steps are: its preparation step is the same as embodiment 1.

[0045] 1. Production and transplantation of test tube agar slant medium:

[0046] ①Preparation of culture medium: Add 2.2g of glucose and 2.2g of agar to 120ml of slag water produced by potato starch production, with a natural pH value. Under the condition of bacteria, it was divided into 15×1.5cm dry and sterilized test tubes, placed on a slope, and set aside after solidification; preparation of sawdust agar slant medium: add 5g of sawdust, glucose 2g, agar 2g and the rest are the same as above.

[0047] ②Transplantation culture: Inoculate the ganoderma lucidum mycelium block in the slant surface of the test tube of the parent species on the slant medium of the test tube and culture it statically at 26°C for 5 days, then transfer it to the slant medium of sawdu...

Embodiment 3

[0053] Embodiment 3: the method for preparing Ganoderma lucidum mycelia fluid with the bad water produced in beer production as a medium, the steps are: the preparation steps are the same as in Example 1.

[0054] 1. Production and transplantation of test tube agar slant medium:

[0055] ①Preparation of culture medium: add 1.8g of glucose and 1.8g of agar to 80ml of bad water produced by beer production, and the pH value is natural. Under the condition of bacterium, put it in a dry and sterilized test tube of 15×1.5cm, place it on an inclined plane, and wait for it to solidify for later use; preparation of sawdust agar inclined medium: add 4g of sawdust and 1.8 of glucose to 80ml of bad water produced by beer production. g, agar 1.8g, and the rest are the same as above.

[0056] ②Transplantation culture: Inoculate the ganoderma lucidum mycelium block in the slant surface of the test tube of the parent species on the slant medium of the test tube and culture it statically at 2...

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Abstract

The invention mainly relates to a method for preparing mycelium liquid by submerged fermentation of lucid ganoderma liquid, in particular to a method for preparing lucid ganoderma mycelium liquid from slag water generated in the production of starch. The method comprises the following steps of: culturing and acclimatizing lucid ganoderma bacteria in a culture medium prepared from slag water generated in production of potato starch, corn starch, bean starch or bear to obtain excellent strain; adding a small amount of sugar and inorganic salts in slag water generated in production of potato starch, corn starch, bean starch or bear; not sterilizing culture materials; inoculating a certain amount of strains; and carrying out aerobic fermentation culture to prepare lucid ganoderma mycelium liquid. According to the method, waste water generated by starch and bear production enterprises is used as raw material, no three waste pollution is not generated in the production process, the production process is simple, and convenient to operate, and energy and manpower can be saved; products can be applied to organic waste water purifying agent, various bioactive matters can be extracted from the products to be used for preparing materials of drugs for adjuvant treatment of tumors, Aids, senile diseases and to be used as an enzymic preparation and a flocculant in oil exploitation, food industry, medical treatment, fine chemical engineering and other industries.

Description

technical field [0001] The invention mainly relates to a method for preparing mycelium liquid by submerged fermentation of ganoderma lucidum liquid, in particular to a method for preparing ganoderma lucidum mycelium liquid by using dross water produced in starch production or beer. Background technique [0002] The traditional method of preparing mycelium liquid by liquid fermentation of Ganoderma lucidum uses a liquid medium prepared from glucose, peptone, yeast extract, soybean flour, corn flour, vitamins, inorganic salts and distilled water. The shredded pieces are inoculated in the liquid medium, and the ganoderma lucidum mycelia liquid is prepared through aerobic fermentation. The disadvantages are that the liquid fermentation medium used has many raw materials, high cost, complex production process, and can not save energy and manpower. Contents of the invention [0003] The purpose of the present invention is to avoid the deficiencies of the prior art and provide a ...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 孙宇孙涛王永帅孙荣高
Owner 甘肃盛亚肽生物科技有限责任公司
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