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Protease extracted by using squid viscera as raw materials and extraction method and application thereof

A technology of squid viscera and protease, which is applied in the direction of hydrolytic enzymes, etc., to achieve the effects of increasing varieties, good stability, and increasing the added value of fishery production

Inactive Publication Date: 2012-12-19
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no technology to extract protease from squid viscera at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The present embodiment utilizes squid viscera to extract protease, and its specific steps are as follows:

[0042] (1) Take 100g of fresh squid viscera and grind it until it becomes muddy with a homogenizer.

[0043] (2) Add 150 mL of 0.5% (mass percent concentration) NaCl solution to extract at room temperature, and the extraction time is 60 min to obtain an extract.

[0044] (3) Centrifuge at 3000r / min for 10min to remove the precipitation of the extract and the grease on the surface, and take the supernatant.

[0045] (4) Use edible acetic acid to adjust the pH value of the supernatant to about 4.5, centrifuge under the same conditions as above, remove the precipitate, and use the supernatant.

[0046] (5) Mix the supernatant obtained in step (4) with 1% (mass percent concentration) ferric sulfate solution in a ratio of 4:1 by volume, then let it settle for 30 minutes, centrifuge at 3000 r / min for 10 minutes to remove the precipitate, and The supernatant is the pro...

Embodiment 2

[0050] (1) Get 100g of fresh squid viscera and grind it until it becomes muddy with a homogenizer.

[0051] (2) Add 200 mL of 0.3% (mass percent concentration) NaCl solution to extract at room temperature, and the extraction time is 60 min to obtain an extract.

[0052] (3) Centrifuge at 3000r / min for 15min to remove the precipitation of the extract and the grease on the surface, and take the supernatant.

[0053] (4) Adjust the pH value of the supernatant obtained in step (3) to about 4.5 with edible acetic acid, centrifuge, and the conditions are the same as in step (3), remove the precipitate, and use the supernatant.

[0054] (5) The supernatant obtained in step (4) is mixed with 1% (mass percent concentration) aluminum sulfate solution in a ratio of 5: 1 by volume, then left to settle for 60 minutes, centrifuged, and the conditions are the same as in step (3), The resulting supernatant is the protease solution.

[0055] (6) Add 2g mannitol to the above-mentioned protease ...

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PUM

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Abstract

The invention discloses protease extracted by using squid viscera as raw materials and an extraction method and an application thereof. According to the invention, fresh or freezed squid viscera are used as raw materials; the squid viscera are crushed, and extracted by water or a table salt solution; the concentration of the table salt solution, the solid-liquid ratio, the temperature, and the extraction time are controlled during the extraction so as to obtain a high protease recovery rate; then protein is removed by methods of isoelectric point precipitation and flocculant addition precipitation so as to increase the protease purity; finally a liquid squid viscera protease enzyme preparation is obtained by concentration, stabilizer and preservative addition, and the like; or a solid squid viscera protease preparation is obtained by filler addition, drying and crushing. The invention realizes the application of squid viscera with increased value, increases the added value of fishery production, and reduces environment pollution caused by squid product production; the prepared squid protease can be used as an enzyme preparation for hydrolyzing various food protein; and the varietyof commercial protease is increased.

Description

technical field [0001] The invention belongs to the field of aquatic products and relates to comprehensive utilization of aquatic waste, in particular to a protease extracted from squid viscera as a raw material, an extraction method and application thereof. Background technique [0002] Generally speaking, the processing by-products composed of internal organs, skin, fins and ink after processing squid to obtain edible parts account for about 30% of the total proportion. The components of the processing by-products are: moisture 63%, crude fat 22.5%, crude protein 13.5%, other 1%. For these wastes, some of them are processed into squid slurry in China, and some of them are treated as garbage and buried on the spot. This is not only a huge waste of fishery resources, but also has hidden dangers of environmental pollution. With the development of modern marine processing industry and aquaculture industry, the comprehensive utilization of squid waste has been paid more and m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/64A23J3/34
Inventor 段杉尹青春
Owner SOUTH CHINA AGRI UNIV
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