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Method for producing Bacillus mucilaginosus liquid microbial inoculant from lignocellulose

A technology of Bacillus colloid and lignocellulose, which is applied in the field of biomass resource utilization and comprehensive utilization of agricultural waste, can solve the problems of unstable spore yield and low number of viable bacteria, and achieve value-added utilization and complete hydrolysis. , the effect of improving spore yield

Inactive Publication Date: 2016-05-11
北京中植科创生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are problems such as low number of viable bacteria and unstable spore yield in the traditional production of Bacillus colioids.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The pretreatment of embodiment 1 lignocellulosic raw material

[0036] Using corn stalks as raw materials, first crush them into 40-60 mesh (280-400um) particles with a pulverizer, make them into a suspension with a concentration of 20% (w / v%) with water, put them in water In a thermal reactor (model CJF-20L, Datong Datong Reactor Factory), treat it at 180°C and 1.2MPa for 10 minutes to loosen the fiber structure of the straw for subsequent processing.

Embodiment 2

[0037] The cellulase enzymatic hydrolysis of embodiment 2 lignocellulosic raw material

[0038] Add cellulase (SDC-A, Ningxia Xiasheng Industrial Group Co., Ltd.) to the suspension obtained in Example 1 according to the amount of 15 FPU / g straw, and react for 48 hours at 55 ° C, pH 4.8, and 100 rpm. The residue is removed by centrifugation to obtain an enzymatic hydrolyzate with a glucose concentration of 50-60 g / L.

Embodiment 3

[0039] Example 3 Lignocellulose enzymolysis liquid fermentation production of colloidal bacillus

[0040] In the enzymatic hydrolyzate of corn stalks obtained in Example 2, after adding dipotassium hydrogen phosphate, magnesium sulfate, ammonium sulfate, and yeast extract components, the colloidal bacillus (purchased from China Agricultural Microorganisms) was inserted according to the dosage of 10%. Preservation Management Center (Agricultural Culture Collection of China, ACCC, preservation number is ACCC19749), at 37 ℃, pH7.0, 200rpm under the conditions of fermentation culture, when the fermentation is carried out for 24h, the number of viable bacteria can reach 3.67 × 10 8 Individual / mL, the yield of spores is more than 85% at this time, and the fermentation is completed, and the thalline is collected by centrifugation.

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Abstract

The invention provides a method for producing a Bacillus mucilaginosus liquid microbial inoculant from lignocellulose. The method comprises the following steps: (1) pulverizing a lignocellulose raw material into 40-60-mesh particles, and carrying out hydrothermal treatment to obtain a suspension; (2) adding cellulase into the suspension obtained in the step (1), carrying out enzymolysis, and centrifugating to remove residues, thereby obtaining a glucose-containing enzymolysis solution; (3) adding culture medium components, inoculating Bacillus mucilaginosus, and culturing at 37 DEG C at the rate of 200 rpm under the pH value of 7.0 to obtain the Bacillus mucilaginosus cells; and (4) adding the Bacillus mucilaginosus cells obtained in the step (3) into an inorganic salt buffer system to obtain the Bacillus mucilaginosus liquid microbial inoculant. After the hydrothermal technique is adopted to pretreat the lignocellulose raw material, the degradation capacity of the cellulose is enhanced. The enzymolysis solution is utilized to perform the fermentation production of the Bacillus mucilaginosus microbial inoculant, thereby enhancing the viable count and implementing the value-added utilization of the lignocellulose.

Description

technical field [0001] The application relates to the fields of utilization of biomass resources and comprehensive utilization of agricultural waste, in particular to a method for producing a liquid Bacillus colloidus agent by using lignocellulosic raw materials. Background technique [0002] my country is a large agricultural production country with abundant lignocellulose raw materials. More than 700 million tons of crop straws are produced every year, among which corn straws, wheat straws and rice straws are the three major types of straws in my country. In addition to a small portion being utilized, hundreds of millions of tons of straw are burned directly or become solid waste every year, resulting in waste of resources and environmental pollution. [0003] Lignocellulosic raw materials account for 35-45% cellulose and 20-40% hemicellulose. If appropriate technology is used to degrade them into fermentable sugars, and then use microorganisms to further ferment to produc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/22C12N1/20C12R1/07
CPCC12N1/22C12N1/20
Inventor 王志芳梁慕姜海苓梁蒙梁敬
Owner 北京中植科创生物技术有限公司
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