This invention relates to a method for extracting
fish oil using
squid viscera, belonging to the field of
biotechnology for oil extraction, and includes the following steps: (1) Rinse fresh
squid viscera with
clean water and crush the viscera into a uniform
slurry for later use; (2)
Grind citrus peel to 40-60 mesh for later use; (3) Add 3-5g of citrus peel, 2-4g of
kombucha, and 1×10⁶-1×10⁸ CFU / g of
Aspergillus spore powder per
kilogram of
slurry, mix evenly, and heat at 28-30℃ at a speed of 80-100 r / min. n, ferment for 18-24 hours; (4) add an appropriate amount of water to the
fermentation product, with a material-to-
water volume ratio of 1:1-1:2, stir evenly, and preheat to the
enzymatic hydrolysis temperature; (5) use
neutral protease and
lipase for compound
enzymatic hydrolysis. After the
enzymatic hydrolysis is completed, raise the temperature of the material to 90-95℃ and maintain it for 10-15 minutes to destroy the
enzyme activity and terminate the reaction; (6) the enzymatic
hydrolysate is separated by a three-phase
centrifuge to obtain three components: the upper layer is crude
squid oil, the lower layer is
protein hydrolysate, and the middle layer is
solid residue. This invention uses the synergistic effect of
fermentation pretreatment and compound enzymatic
hydrolysis to significantly improve the
fish oil extraction rate and protect its quality.