Biological active early warning marking method for chemical industry wastewater treatment
A bioactive, chemical wastewater technology, applied in the field of environmental microbial molecular ecology, can solve the problems of no patent research report, inability to effectively monitor the operation of the sewage treatment system, etc., to ensure continuous and stable operation, easy and fast operation, saving manpower and material resources Effect
Inactive Publication Date: 2012-04-11
NANKAI UNIV
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Problems solved by technology
The above-mentioned patents are respectively related to the detection method of functional bacteria in sewage treatment and the analysis method of microbial community composition, which have a positive effect on promoting the stable operation of the sewage treatment system, but have not combined the change of community structure in the sewage treatment system with the detection of functional bacteria in the application sewage treatment system
The sewage treatment system operates as a whole, and the operation of the sewage treatment system cannot be effectively monitored only by analyzing the composition of functional bacteria or microbial community structures in the system
At present, there are no patent research reports on microbial community structure and functional bacteria in wastewater treatment
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Embodiment 1
[0037] The following is a specific application example to illustrate the chemical wastewater treatment system Biological Activity Early Warning Marking Method ,details as follows:
[0038] 1. Extraction of microbial genome total DNA from activated sludge in each process section of the comprehensive chemical wastewater treatment system, and strain screening and identification.
[0039] (1) Extraction of total DNA
[0040] The activated sludge samples were collected from the hydrolytic acidification tank, anoxic tank and aerobic tank of the sewage treatment plant in Hangu Industrial Park, Binhai New Area, Tianjin. After the sample was collected, the genome was extracted according to the following methods: ① Sample pretreatment: Measure 30 mL of activated sludge sample, centrifuge at 10,000 rpm, 4°C for 5 min, and discard the supernatant. Add 100mL TENP buffer (50mM Tris, 20mM EDTA, 100mM NaCl, 1%PVP, pH=10) to the precipitation, add 4 sterilized glass beads (diameter 6mm), vo...
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The invention relates to a biological active early warning marking method for chemical industry wastewater treatment, which specifically comprises the following steps of: extracting microorganism genome total DNA in active sludge of a sewage treatment system, and screening bacterial seeds by enrichment culture method and identifying; amplifying the microorganism genome total DNA by universal primers of V6-V8 areas of bacterial 16SrDNA; carrying out PCR-DGGE (Polymerase Chain Reaction Denaturing Gradient Gel Electrophoresis) analysis to the PCR product, and creating community succession mode in normal running system; finding bacterial strains closely related with COD (Chemical Oxygen Demand) change in the system, and using RAPD (Random Amplified Polymorphic DNA) technique to obtain specific molecular marker genes of pure culture strains; and directly judging if functional bacteria are existent for monitoring the sewage treatment system by PCR of specific molecular marker genes of functional bacteria. The invention solves the difficult problem that the current sewage treatment system cannot effectively and stably run, and the method is simple, convenient and fast to operate and can effectively monitor running situation of the sewage treatment system.
Description
technical field [0001] The invention relates to a biological activity early warning marking method for chemical wastewater treatment, which can be applied to the monitoring of the operation of sewage treatment systems, and belongs to the field of environmental microbial molecular ecology. Background technique [0002] The biological treatment of wastewater does not require the addition of chemicals, and there is no secondary pollution; under mild conditions, it can be completed efficiently and relatively thoroughly through enzyme catalysis, and the treatment cost is low; the requirements for wastewater quality are broad; the treatment effect is good, not only removing organic matter and pathogens , toxic substances, etc., can also remove odor, improve transparency, reduce chroma, etc. For the sewage treatment system, the stable operation of the sewage treatment system is usually ensured by monitoring the water quality, such as controlling and monitoring the chemical paramete...
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IPC IPC(8): C02F3/34
Inventor 马挺吕晶华李国强
Owner NANKAI UNIV
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