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Fungus for degrading phthalate ester and application thereof

A phthalate ester and fungus technology, applied in the field of biological treatment of environmental organic pollutants, to achieve the effect of high-efficiency degradation ability

Inactive Publication Date: 2012-04-11
SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the existing PAEs polluted soil and water environment restoration technology, the primary purpose of the present invention is to provide a fungus that can efficiently and rapidly degrade PAEs

Method used

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  • Fungus for degrading phthalate ester and application thereof
  • Fungus for degrading phthalate ester and application thereof
  • Fungus for degrading phthalate ester and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Isaria sp. F 3 Screening, isolation and identification and its terephthalate degradation performance

[0020] Weigh 10g of fresh paddy soil contaminated by PAEs, put it into a 250mL Erlenmeyer flask with 90mL of deionized water and 3g of glass beads, shake at 30°C and 180r / min for 30min, and let it stand for 30min. Then get 10mL supernatant, add the fungus culture medium (MYEA) containing 140mL PAEs (its formula is: malt extract 0.2%; Yeast extract 0.2%, balance is 0.02% chloramphenicol solution, adjust pH value It can be 7.0; if it is made into a solid medium, 2% agar is added; the percentage is the mass-to-volume ratio) to carry out gradient acclimatization for 3 times. The concentrations of the three PAEs (DMP, DEP and DOP) in each gradient were 200, 400, and 600 mg / L (the total concentration of PAEs was 600, 1200, and 1800 mg / L), and each time 10 mL of bacterial liquid was transferred into 140 mL of fresh medium. Cultivate at 30°C and 120r / min for 7 days, and add ...

Embodiment 2

[0024] Isaria sp. F 3 Terephthalate Degradation

[0025] The selected fungi were picked from the inoculation loop, respectively inoculated with 5 g / L glucose solution, and cultured on a shaking table at 30°C and 120 r / min for 36 hours to induce spore germination and hyphal growth. Spectrophotometer measures the OD value at 660nm of the bacterial solution, and dilutes appropriately to make the concentration consistent (OD 660 ≈0.8). Add 10 mL of bacterial liquid to 90 mL of PAEs inorganic salt medium (the concentrations of the three PAEs are 0, 25, 50, 75, and 100 mg / L, respectively, and the total concentration is 0, 75, 150, 225, and 300 mg / L), at 25°C, 120r / Min cultured for 7 days, the non-biodegradable control was the non-inoculated PAEs inorganic salt medium. Three parallel samples were performed for each treatment, all operated under sterile conditions. After 7 days, samples were taken to analyze the PAHs content and biomass in the culture medium. See the experimenta...

Embodiment 3

[0029] Effects of growth factors on Isaria sp. F 3 Influence of degradation effect

[0030] 1. Select PAEs concentration, C:N and pH as influencing factors ((NH4 ) 2 SO 4 as N source), designed as L 27 (3 3 ) orthogonal experiment, the table header is shown in Table 1.

[0031] 2. Insert the same number of dominant fungal spores (OD 660 ≈0.8), set 3 repetitions; non-inoculated PAEs inorganic salt medium was used as control. Shaking culture (120rpm, 25°C) for 7 days, the degradation rate of ΣPAEs and the pH value of the bacterial solution were measured, and the results are shown in Table 2.

[0032] The preparation method of above-mentioned inorganic salt culture medium is (g·L -1 ): KH 2 PO 4 1.0g / L, (NH 4 ) 2 SO 4 1.0g / L, MgSO 4 ·7H 2 O 0.2g / L, CaCl 2 0.1g / L, FeCl 3 0.01g / L, pH 7.0; distilled water to 1.0L, pH 7.0, steam sterilization at 121°C for 30min.

[0033] The results in Table 2 show that: Isaria sp. F 3 The degradation effect on PAEs is better wh...

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Abstract

The invention belongs to the technical field of biological treatment of environmental organic pollutant and discloses a fungus for degrading phthalate ester and an application thereof in biological treatment of polluted soil and in environmental pollution remediation. The fungus for degrading phthalate ester is bundle pullulan (Isaria sp.) F3 with a preservation number of CCTCC No. M 2011299. The bundle pullulan (Isaria sp.) F3 has a total degrading rate of 52.7-66.2% in the mixed system of DMP (dimethyl phthalate), DEP (diethyl phthalate) and DOP (dinoctyl orthophthalate) within 7 days under a culturing condition; wherein the degrading rate of DMP is 61.5-78.4%, the degrading rate of DEP is 53.7-68.1%, and the degrading rate of DOP is 29.5-53.7%. The 300 mg / kg of PAEs in the sterilized soil is degraded to 69.0% within 30 days; the efficiency for soil phytoremediation polluted by DMP, DEP and DOP can be obviously improved. Compared to other strains, the fungus has efficient phthalate ester degradation capacity.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental organic pollutants, and in particular relates to a fungus strain degrading phthalate and its application in contaminated soil biological treatment and environmental pollution restoration. Background technique [0002] Phthalic acid esters, also known as phthalic acid esters (PAEs), are composed of a rigid planar aromatic hydrocarbon and two plastic non-linear aliphatic side chains, usually esters between phthalic anhydride and various alcohols The chemical reaction is obtained. [0003] PAEs widely exist in water, soil and air. With the widespread agricultural use of plastic films and the discharge of a large amount of urban sewage and garbage into farmland, phthalic acid esters (PAEs) have become one of the most common organic pollutants in farmland soil, and are the "second global pollutant". PCB Contaminants". Due to the estrogenic effects of PAEs, as well as te...

Claims

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Application Information

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IPC IPC(8): C12N1/14A62D3/02B09C1/10C12R1/645A62D101/28
Inventor 蔡信德郭杨韩蕊杜文婷杨子江李诗殷
Owner SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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