Method for detecting heavy metal by utilizing molecular device based on graphene electrode

Abstract

The invention discloses a method for detecting heavy metal and a DNAzyme monomolecular connection device based on a graphene electrode special for same. A manufacturing method for the DNAzyme monomolecular connection device comprises the following steps of: 1) constructing a graphene transistor device array; 2) cutting a nanometer gap of 1-10nm in a graphene molecular device; and 3) connecting a designed DNAzyme sequence into the nanometer gap to obtain the DNAzyme monomolecular connection device. An electrical signal detection with heavy metal ion binding and identification is carried out byutilizing the manufactured DNAzyme monomolecular connection device, meanwhile, the interference factors are excluded; the heavy metal ions are tested in sensitivity and the like on the connection device; and the measurement conditions are determined according to the test results in the above steps, and the heavy metal ions are detected on the connection device. According to the invention, the high-sensitivity detection of the heavy metal can be realized through the electricity signal by utilizing the functionalized graphene electrode molecular device. The method is of great significance in detecting a trace amount of the heavy metal in the environment.

Description

technical field

[0001] The invention relates to a method for detecting heavy metals using a graphene electrode-based molecular device. Background technique

[0002] Heavy metals can not only exist for a long time, but also can be stored in organisms through the accumulation of food chains. The toxicity produced is difficult to detect. Moreover, the pollution of heavy metals is becoming more and more serious, which has attracted people's attention in medicine, food, environment and other aspects. At present, there are more and more researches on heavy metal detection methods, mainly including immunoassay, atomic spectrophotometry, stripping voltammetry, chromatography, test paper method, enzyme analysis method, biochemical sensor method, etc. Some existing methods lack Higher sensitivity is greatly affected by environmental factors, and it is urgently needed to establish a system of highly sensitive detection methods. (1: Malitesta C, Guascito M R. Biosensor Bioelectron, 200...

Images