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Method for extracting nuciferine from lotus leaves

A technology of nuciferine and lotus leaf, applied in the field of separation of natural products, can solve the problems of low product content, poor quality, unsuitable for industrialization, etc., and achieve the effect of low energy consumption and good product quality

Inactive Publication Date: 2012-05-09
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, the existing technology has many shortcomings such as high extraction cost, low product content, poor quality or unsuitable for industrialization.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Grind the lotus leaf raw material, take 500g, add 6L of 1% hydrochloric acid aqueous solution, heat to 60°C to extract for 3 hours, filter, add 5L of 1% hydrochloric acid aqueous solution to the filter residue, extract at 60°C for 2 hours, filter, add the filter residue Extract 4L of 1% hydrochloric acid aqueous solution at 60°C for 1 hour, filter, combine the filtrate, pass the filtrate through the ADS-21 macroporous resin column, add the column liquid directly to the 0.3L 732 cationic resin column, wash the cationic resin to the bottom The column liquid is neutral, and then eluted with 1.2L of 6% sodium chloride solution, and the lower column liquid is collected. The lower column liquid passes through the nanofiltration membrane with a molecular weight cut-off of 200, and the inlet pressure is controlled at 0.6-0.8MPa. solution, the concentrated solution was adjusted to pH 8.5 with 3% NaOH solution, and the precipitate was filtered. The precipitate was refluxed with 80...

Embodiment 2

[0025] Grind the lotus leaf raw material, take 5Kg, add 60L of 1% hydrochloric acid aqueous solution, heat to 55°C for extraction for 3 hours, filter, add 50L of 1% hydrochloric acid aqueous solution to the filter residue, extract for 2 hours at 55°C, filter, add the filter residue 40L of 1% hydrochloric acid aqueous solution was extracted at 55°C for 2 hours, filtered, and the filtrates were combined. The filtrate passed through the AB-8 type macroporous resin column, and the next column liquid directly passed through the 3L D001 type cation resin column, and washed the cation resin to The lower column liquid is neutral, and then eluted with 18L of 8% sodium chloride solution, and the lower column liquid is collected. The lower column liquid passes through the nanofiltration membrane with a molecular weight cut-off of 200, and the inlet pressure is controlled at 0.6-0.8MPa. liquid, the concentrated solution was adjusted to pH 9 with 10% ammonia solution, and the precipitate wa...

Embodiment 3

[0027] Grind the lotus leaf raw material, take 10Kg, add 90L of 1% sulfuric acid aqueous solution, heat to 58°C for extraction for 3 hours, filter, add 84L of 1% sulfuric acid aqueous solution to the filter residue, extract for 3 hours at 58°C, and filter , add 80L of 1% sulfuric acid aqueous solution to the filter residue, extract at 55°C for 2 hours, filter and combine the filtrate, the filtrate passes through the NKA-II macroporous resin column, the next column liquid directly passes through the 6L 732 type cation resin column, and washes the cation The resin is neutral until the lower column liquid is eluted with 30L of 7% sodium chloride solution, and the lower column liquid is collected. The lower column liquid passes through a nanofiltration membrane with a molecular weight cut-off of 200, and the inlet pressure is controlled at 0.5-0.8MPa. Concentrate to obtain a concentrated solution, which is adjusted to pH 9.5 by adding 5% KOH solution to the concentrated solution, f...

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PUM

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Abstract

The invention provides a method for extracting nuciferine from lotus leaves, which is characterized by comprising the following steps: crushing lotus leaf materials; adding an acidic water solution the amount of which is 8-15 times that of the materials; extracting for 2-3 times while heating; adding the extracting solution into a macroporous resin column and a cation resin column; washing with water until the cation resin is neutral; eluting with 4-7 times of sodium chloride solution to remove active components, thus obtaining an eluent; adding a nanofiltration membrane, and concentrating to obtain a concentrated solution; and regulating the pH value to 8-10, precipitating, crystallizing the precipitate with ethanol for 2-3 times, and drying the obtain the product. The process has the advantages of simple operation, low energy consumption and less pollution in the production process, high product content and no residue of organic solvent, thereby being suitable for industrial popularization.

Description

Technical field: [0001] The invention belongs to the field of separation of natural products, in particular to a method for extracting nuciferine from lotus leaves. Background technique: [0002] Nuciferine is a kind of aporphine alkaloid, hydrophobic weak base. [0003] Molecular formula: C 19 h 21 NO 2 . Molecular weight: 295.38. [0004] Nuciferine is the main lipid-lowering active ingredient in lotus leaves, which has medicinal and dietary effects such as anti-free radicals, inhibition of hypercholesterolemia and arteriosclerosis. [0005] The lotus leaf is the dry leaf of lotus (Nelumbo nucifera Gaertn.), a plant of the water lily family. Traditional Chinese medicine believes that the lotus leaf is bitter in nature and flavor, flat, and returns to the liver, spleen, stomach, and heart meridian. It can treat headache, dizziness, edema, abdominal distension due to lack of food, diarrhea, leucorrhea, prolapse of the anus, vomiting blood, epistaxis, hemoptysis, blood ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D221/18
Inventor 李法庆杨成东刘东锋
Owner 苏州宝泽堂医药科技有限公司
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