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Ginsenoside Re extraction and separation method

A technology for extracting ginsenosides and solvents, which is applied in the field of extraction and separation of ginsenoside Re, can solve the problems of not being suitable for industrial production, and achieve the effects of environmental protection, low production cost, and short cycle

Inactive Publication Date: 2012-05-23
CHANGCHUN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method is characterized in that ginsenoside Re is separated by macroporous adsorption resin or HSCCC technology, and the starting material is ginseng or ginseng energy extract. Although the obtained sample has high purity, it is not suitable for industrial production

Method used

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  • Ginsenoside Re extraction and separation method
  • Ginsenoside Re extraction and separation method
  • Ginsenoside Re extraction and separation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Soaking and decoction of raw materials

[0031] Add the 60% ethanol solution into a steam distillation device at a weight ratio of raw material to solution of 1:15, soak at room temperature for 1 hour, heat to keep it in a slightly boiling state for 3 hours, filter while hot, collect the filtrate, and repeat extraction The operation is once, the extraction time is 0.5 hours, and the filtrates are combined.

[0032] 2. Concentration of extract

[0033] The filtrate obtained in step 1 was concentrated to 1 / 3 of the original extract volume under reduced pressure.

[0034] 3. Ethanol precipitation

[0035] Add 3 times the volume of ethanol solution to the concentrated solution obtained in step 2 for precipitation. The ethanol concentration is 30%. After standing for 24 hours, filter and collect the filtrate.

[0036] 4. Reconcentration of the extract

[0037] The filtrate obtained in step 3 is again concentrated under reduced pressure to 1 / 4 of the original volume.

[0038] 5. Organ...

Embodiment 2

[0043] Soaking and decoction of raw materials

[0044] Add the 90% ethanol solution into the steam distillation device at a weight ratio of raw material to solution of 1:5, soak at room temperature for 1 hour, heat to keep it in a slightly boiling state for 1 hour, filter while hot, collect the filtrate, and repeat extraction The operation was performed twice, the extraction time was 0.5 hours, and the filtrate was combined.

[0045] Concentration of extract

[0046] The filtrate obtained in step 1 was concentrated to 1 / 4 of the original extract volume under reduced pressure.

[0047] Ethanol precipitation

[0048] Add 3 times the volume of ethanol solution to the concentrated solution obtained in step 2 for precipitation. The ethanol concentration is 60%. After standing for 24 hours, filter and collect the filtrate.

[0049] Reconcentration of extract

[0050] The filtrate obtained in step 3 is again concentrated under reduced pressure to 1 / 4 of the original volume.

[0051] Organic solv...

Embodiment 3

[0056] Sample liquid chromatography identification:

[0057] Apparatus and chromatographic conditions: high performance liquid chromatograph Agilent 1200SL, DAD detector, Chemstation workstation, ginsenoside Re reference substance purchased from China Pharmaceutical and Biological Products Laboratory (batch number: 110754-200822), chromatographic column: SDBC 18 1.8μm, 4.8×50mm, using acetonitrile-water as mobile phase, gradient elution, flow rate 1ml·min -1 , Detection wavelength is 203nm, column temperature is 25℃.

[0058] Test result: Calculated by normalization method with peak area as an index, the content of ginsenoside Re accounted for more than 80% of the total saponins.

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Abstract

The invention discloses a ginsenoside Re extraction and separation method. The method is characterized in that raw materials used in the extraction method are from buds, stems, leaves and fruits of ginseng, American ginseng, Korean ginseng and the like. The extraction steps comprise ethanol refluxing, condensation, alcohol precipitation, organic solvent extraction, standing, filtering, drying and the like. The extraction method is characterized by comprising steps of extraction of the raw materials to obtain an extract product, condensation, standing, filtering and the like. After filtering, a white powder is obtained. By the utilization of saponin solubility and content difference, one-step extraction and separation is realized. Macroporous adsorption resin or HSCCC technology is not utilized for separation. By HPLC detection of the product obtained after separation, the peak area is used as an index to calculate that the Re content accounts for more than 80% of the total saponin content by a normalization method. By the adoption of the method provided by the invention, a new idea for industrial extraction of ginsenoside can be developed.

Description

Technical field: [0001] The invention relates to a method for extracting and separating ginsenoside Re and belongs to the field of medicine and health care. Background technique: [0002] Ginsenoside Re (ginsenoside-Re) is one of the ginsenosides in the ginsenosides, and one of the most abundant saponins in ginseng, especially in flowers, stems and leaves. Modern pharmacological research has proved the pharmacological activity of ginsenoside Re in anti-oxidative stress damage, improving immunity, anti-cancer, nootropics, etc., and explained its pharmacological mechanism from the molecular level, which not only corroborates the ancient Chinese medical books The usage of ginseng, and further suggests that ginsenoside Re may have broad application prospects in the treatment of myocardial infarction, treatment of senile dementia, and promotion of intellectual development. [0003] Due to the complex structure of ginsenosides, it is still impossible to synthesize any kind of saponins m...

Claims

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Application Information

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IPC IPC(8): C07J17/00
Inventor 李慧贾冰刘淑莹
Owner CHANGCHUN UNIV OF CHINESE MEDICINE
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