Preparation method and application of Clenbuterol surface molecularly imprinted material

A surface molecular imprinting and reaction technology, which is applied in chemical instruments and methods, ion exchange, and other chemical processes, can solve the problems of too deep embedding of template molecules, irregular shapes, and low mechanical properties, and the adsorption equilibrium time is short , Simple preparation process and short elution time

Inactive Publication Date: 2012-06-13
杨挺
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The bulk polymerization method is currently the most widely used and the most mature and versatile method for preparing imprinted polymers. Its remarkable advantages are that the preparation process is relatively simple and the polymerization system is easy to control. Disadvantages such as leakage, irregular shape, and low mechanical properties have largely inhibited the application in real samples.

Method used

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  • Preparation method and application of Clenbuterol surface molecularly imprinted material
  • Preparation method and application of Clenbuterol surface molecularly imprinted material
  • Preparation method and application of Clenbuterol surface molecularly imprinted material

Examples

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Effect test

Embodiment 1

[0029] Weigh 30 g of silica gel (particle size 5 μm), add 150 mL of 10% methanesulfonic acid aqueous solution, stir at reflux temperature for 8 h, wash with distilled water until neutral, and dry at 65° C. for 12 h to obtain activated silica gel. Add 10g of activated silica gel to 400mL of water, add 10mL of 3-(methacryloyloxy)propyltrimethoxysilane, stir and react at 50°C for 24h, wash the product with water and ethanol, and dry at 65°C for 12h to obtain a double bond Composite silicone. Add 200mL of water and 3g of double-bonded silica gel into the flask, stir for 1h, then add 10mL of methacrylic acid, stir for 1h under the protection of N2, add 1mL of 20% ammonium persulfate solution, heat up to 70°C, stir for graft polymerization After 24 hours of reaction, grafted particles were obtained. Soxhlet extraction with ethanol for 24 hours, and then dried at 65°C for 12 hours before use. Take 50mL concentration as 1000mg·L -1 Clenbuterol aqueous solution, add 1g grafted parti...

Embodiment 2

[0036] Weigh 30 g of silica gel (particle size 40 μm), add 150 mL of 10% methanesulfonic acid aqueous solution, stir at reflux temperature for 8 h, wash with distilled water until neutral, and dry at 65° C. for 12 h to obtain activated silica gel. Add 5g of activated silica gel to 200mL of water, add 5mL of 3-(methacryloyloxy)propyltrimethoxysilane, stir and react at 40°C for 24h, wash the product with water and ethanol, and dry at 65°C for 12h to obtain a double bond Composite silicone. Add 200mL of water and 3g of double-bonded silica gel into the flask, stir for 1h, then add 10mL of methacrylic acid, stir for 1h under the protection of N2, add 1mL of 10% ammonium persulfate solution, heat up to 70°C, stir for graft polymerization After 24 hours of reaction, grafted particles were obtained. Soxhlet extraction with ethanol for 24 hours, and then dried at 65°C for 12 hours before use. Take 50mL concentration as 1000mg·L -1 Clenbuterol aqueous solution, add 1g grafted partic...

Embodiment 3

[0038] Weigh 30 g of silica gel (particle size 120 μm), add 150 mL of 20% hydrochloric acid aqueous solution, stir at reflux temperature for 8 h, wash with distilled water until neutral, and dry at 65° C. for 12 h to obtain activated silica gel. Add 5g of activated silica gel to 200mL of water, add 5mL of 3-(methacryloyloxy)propyltrimethoxysilane, stir and react at 60°C for 24h, wash the product with water and ethanol, and dry at 65°C for 12h to obtain a double bond Composite silicone. Add 200mL of water and 3g of double-bonded silica gel into the flask, stir for 1h, then add 10mL of methacrylic acid, stir for 1h under the protection of N2, add 1mL of 10% ammonium persulfate solution, heat up to 70°C, stir for graft polymerization After 24 hours of reaction, grafted particles were obtained. Soxhlet extraction with ethanol for 24 hours, and then dried at 65°C for 12 hours before use. Take 50mL of clenbuterol aqueous solution with a concentration of 500mg L-1, add 1g of grafte...

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Abstract

The invention relates to a method for preparing a Clenbuterol surface molecularly imprinted material. The method is characterized by comprising the following steps of: (1) activating silica gel; (2) bonding the double bond of the silica gel; (3) performing surface chemical grafting on the silica gel; and (4) preparing a product. The invention also discloses application of the surface molecularly imprinted material to the detection of Clenbuterol residues. Compared with the prior art, the method has the advantages that: the preparation process of the Clenbuterol surface molecularly imprinted material is simple and high in operability, and preparation cost is low; and the surface molecularly imprinted material is high in specificity, short in Clenbuterol adsorption balance time and elution time and good in elution effect.

Description

technical field [0001] The invention relates to a method for preparing a surface molecularly imprinted material, in particular to a method for preparing a surface molecularly imprinted material capable of detecting clenbuterol residues. The invention also discloses the application of the surface molecular imprinting material. Background technique [0002] Clenbuterol is a selective adrenergic receptor agonist, commonly known as "clenbuterol", which can easily cause accumulation in animal eyes, hair, liver, lung, kidney and other tissues. After eating food containing clenbuterol, humans will have serious side effects. The main symptoms are rapid heartbeat, trembling limbs, abdominal pain, dizziness, dyspnea, nausea and vomiting. Long-term consumption of foods containing clenbuterol can cause diseases of the human cardiovascular system and nervous system, and even cause chromosomal aberrations and induce malignant tumors. [0003] The serious harm caused by clenbuterol resid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/30B01J20/26B01D15/08
Inventor 杨挺吴银良陈国皇甫伟国
Owner 杨挺
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