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Method for measuring water-soluble nitrogen content in plants and plant products

A technology for plant products and determination methods, applied in the direction of color/spectral property measurement, etc., can solve the problems of incomplete and thorough digestion products, low recovery rate of water-soluble nitrogen, inaccurate determination results, etc., and achieves simple steps and short processing cycles. , the result is accurate

Active Publication Date: 2013-11-27
CHINA TOBACCO GUANGDONG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] This high-pressure digestion has a good digestion effect on inorganic nitrogen, but for plant alkaloids, amino acids, water-soluble protein nitrogen and other water-soluble nitrogen compounds, such as nicotine, theophylline, valine, etc., due to their relatively Complicated, incomplete and incomplete digestion occurs when high-pressure digestion is used, resulting in low recovery of water-soluble nitrogen determination and inaccurate measurement results
For plants and plant samples, the water-soluble nitrogen is mainly plant alkaloids, amino acids, and water-soluble protein nitrogen, and plants and plant products also contain reducing components, such as sugars, which will react with oxidants , thereby affecting the digestion of nitrogen-containing components. When the above method is used to measure the water-soluble nitrogen, the digestion products obtained are not complete and thorough, resulting in inaccurate measurement results.

Method used

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  • Method for measuring water-soluble nitrogen content in plants and plant products
  • Method for measuring water-soluble nitrogen content in plants and plant products
  • Method for measuring water-soluble nitrogen content in plants and plant products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] 11 g of sodium nitrate was dissolved in 100 mL of distilled water to obtain a sodium nitrate stock solution with a mass concentration of 10.0%. Accurately pipette 1.0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, and 10.0mL of the standard stock solutions, and dilute to 100mL with water respectively to obtain standard sodium nitrate solutions of serial concentrations. Take 2 mL of the standard solution to be tested respectively, pour it into the sample tube of the continuous flow analyzer and measure it in parallel three times respectively, and get the average value. In the continuous flow analyzer, the sample injection volume is 0.1mL / min, the injection time is 100s, and the flushing time is 120s. First at 90°C, add 2mL of potassium persulfate solution with a molar concentration of 0.3mol / L in the standard solution to be tested, the injection volume of potassium persulfate solution is 0.8mL / min, then the mixed solution obtained at 90 ℃, under the acidic condition provided by hydroch...

Embodiment 2～3

[0079] 3.2446g of nicotine was dissolved with distilled water and the volume was adjusted to 200mL to obtain a nicotine stock solution with a mass concentration of 1.60%. Pipette 16.0 mL and 32.0 mL of the nicotine stock solution, and distill the volume to 100 mL with distilled water to obtain a nicotine solution to be tested. Pipette 2.0 mL of the two different concentrations of nicotine to be tested, pour them into the sample tubes of the continuous flow analyzer and measure in parallel three times respectively, and take the average value. In the continuous flow analyzer, the sample injection volume is 0.1mL / min, the injection time is 100s, and the flushing time is 120s. First at 90°C, 2mL of potassium persulfate solution with a molar concentration of 0.3mol / L was added to the nicotine solution to be tested, and the injection volume of the potassium persulfate solution was 0.8mL / min, and then the mixed solution obtained was Under the acidic conditions provided by hydrochlor...

Embodiment 4

[0088] 3.6032g of theophylline was dissolved in distilled water and adjusted to 100mL to obtain a stock solution of theophylline with a mass concentration of 3.48%. Pipette 5 mL of the theophylline stock solution, and distill the volume to 100 mL with distilled water to obtain the theophylline solution to be tested. Pipette 2.0mL described theophylline solution to be tested, pour into the sample tube of continuous flow analyzer and carry out three parallel measurements, get its average value, detection process is identical with the detection process of embodiment 2~3, obtains the ultraviolet ray of theophylline Spectrum.

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Abstract

The invention provides a method for measuring water-soluble nitrogen content in plants and plant products, which includes the following steps: extracting water-soluble nitrogen in a plant and a plant product to obtain a to-be-measured sample containing water-soluble nitrogen; adding potassium persulfate into the to-be-measured sample to obtain a mixed solution under the heating condition; performing ultraviolet catalysis and high-temperature heating to the mixed solution sequentially, so as to obtain a digestion product; detecting the digestion product to obtain ultraviolet spectrum data; and obtaining the water-soluble nitrogen content in the plant and the plant product as per the ultraviolet spectrum data and a predetermined standard curve. Through the adoption of the method provided by the invention, the digestion of the water-soluble nitrogen in the plant and the plant product is more complete, the digestion of the water-soluble nitrogen as well as the digestion of the reductive ingredient in the plant and the plant product can be realized, the reductive function of the to-be-measured sample is lost, the interference in the water-soluble nitrogen ingredient digestion is reduced, so that the digestion effect becomes more uniform, the recovery rate of the water-soluble nitrogen measurement is improved, and the accuracy of the measurement result is improved.

Description

technical field [0001] The invention relates to the technical field of analysis and determination of nitrogen content, in particular to a method for determining the content of water-soluble nitrogen in plants and plant products. Background technique [0002] Water-soluble nitrogen in plants and plant products, including non-protein nitrogen and protein nitrogen. Among them, amino acids in water-soluble protein or water-soluble non-protein nitrogen and reducing sugar components are prone to Maillard (Maillard, also known as Maillard, Maillard, Mena or carbonyl amide) reaction, resulting in brown-black large The molecular substance melanoidin also produces hundreds of intermediate molecules with different odors, including reducing ketones, aldehydes and heterocyclic compounds, etc. These substances provide food with a pleasant and delicious flavor and attractive color, so , the content of water-soluble nitrogen has an important influence on the quality of plants and plant pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33
Inventor 孔浩辉程志颖
Owner CHINA TOBACCO GUANGDONG IND
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