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Method for preparing saccharomycete surface atom transfer imprinted adsorbent

A technology of imprinted adsorption and atom transfer, applied in chemical instruments and methods, alkali metal oxides/hydroxides, inorganic chemistry, etc., to achieve the effect of less by-products, high activity and rapid reaction

Inactive Publication Date: 2014-05-28
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the preparation of imprinted polymeric adsorbents by imprinting on the surface of yeast by atom transfer radical polymerization.

Method used

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  • Method for preparing saccharomycete surface atom transfer imprinted adsorbent
  • Method for preparing saccharomycete surface atom transfer imprinted adsorbent
  • Method for preparing saccharomycete surface atom transfer imprinted adsorbent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Preparation of yeast surface-loaded initiator

[0030] Saccharomyces cerevisiae was dispersed in a mixed solution of tetrahydrofuran and anhydrous triethylamine with a volume ratio of 25:1 (mL), wherein the yeast and anhydrous triethylamine were 0.1 g:1 mL by mass and volume, and kept in ice Nitrogen was passed through the bath to evacuate oxygen, and isobutyryl bromide with a volume ratio of 0.5:1 (mL) to anhydrous triethylamine was added dropwise, reacted at room temperature for 12 h, washed with ethanol three times, and dried in vacuum at 20 °C to obtain Yeast Initiator.

[0031] (2) Preparation of yeast surface imprinted adsorbents (MIPs)

[0032] Add the template molecule cephalexin to the mixed solution of methacrylic acid and ethylene glycol di(methacrylate) ester at a volume ratio of 1:6 (mL), wherein the mass of cephalexin and methacrylic acid The ratio is 0.1:1 (g), and then added to the mixed solution of methanol and distilled water with a volume ratio...

Embodiment 2

[0040] (1) Preparation of yeast surface-loaded initiator

[0041] Saccharomyces cerevisiae was dispersed in a mixed solution of tetrahydrofuran and anhydrous triethylamine with a volume ratio of 45:1 (mL), wherein the yeast and anhydrous triethylamine were 2 g:1 mL by mass and volume, and placed in ice After purging oxygen in the bath with nitrogen ， Add isobutyryl bromide with a volume ratio of 2.5:1 (mL) to anhydrous triethylamine dropwise, react at room temperature for 18 h, wash with ethanol three times, and vacuum-dry at 40 °C to obtain the yeast initiator.

[0042] (2) Preparation of yeast surface imprinted adsorbents (MIPs)

[0043] Add the template molecule cephalexin to the mixed solution of methacrylic acid and ethylene glycol di(methacrylate) ester at a volume ratio of 1:20 (mL), wherein the mass of cephalexin and methacrylic acid The ratio is 1.5:1 (g). Then add the mixed solution of methanol and distilled water with a volume ratio of 6:1 (mL), wherein the volu...

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Abstract

The invention relates to a method for preparing a saccharomycete surface atom transfer imprinted adsorbent and belongs to the technical field of the preparation of environmental materials. The atom transfer radical polymerization process is performed. The method comprises the following steps of: loading an initiator onto the surfaces of saccharomycetes to obtain a matrix material with the initiator, and preparing the saccharomycete surface imprinted adsorbent by taking cefalexin as template molecules, methacrylic acid as a functional monomer, ethylene glycol (methacrylic acid) diester as a cross-linking agent, and CuCl as a catalyst. The spherical imprinted adsorbent has remarkable thermal and magnetic stability. The research on the action between the template molecules and the functional monomer is conducted by using ultraviolet rays and shows that acting force exists. The static adsorption experiment is used for researching the adsorption equilibrium, dynamics and selective recognition performance of the prepared imprinted adsorbent. The result shows that the obtained saccharomycete surface imprinted adsorbent has relatively high adsorption capacity, quick absorption kinetic properties and remarkable cefalexin molecular recognition performance.

Description

technical field [0001] The invention relates to an atomic transfer imprinting adsorbent on the surface of yeast, a preparation method and an application thereof, and belongs to the technical field of environmental material preparation. Background technique [0002] Molecular imprinting technology uses the target analyte as a template molecule, combines structurally complementary functionalized polymer monomers with the template molecule through covalent or non-covalent bonds, and adds a cross-linking agent for polymerization reaction. After the reaction is completed The template molecules are eluted to form a technology of molecularly imprinted polymers (MIPs) with fixed hole size and shape and definite arrangement of functional groups. Surface imprinting technology, by establishing molecular recognition sites on the surface of the matrix material, has better solved some serious defects in the traditional molecular imprinting technology, such as deep embedding of active site...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F222/14C08F220/06C08J9/26B01J20/26B01J20/28B01J20/30
Inventor 李秀秀潘建明戴江栋孟敏佳徐龙城李春香闫永胜
Owner JIANGSU UNIV
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