Method for preparing antarctic krill low-fluorine hydrolysis polypeptide

A technology of Antarctic krill and Antarctic krill meal, applied in the direction of fermentation, can solve problems such as unsatisfactory comprehensive effect, waste of energy, calcium residue, etc., achieve high practical application value, simple and easy operation, and small amount of calcium oxide Effect

Inactive Publication Date: 2012-07-11
辽渔集团有限公司
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AI Technical Summary

Problems solved by technology

[0003] However, there is a problem of high fluorine content in Antarctic krill. Ocean University of China and Dalian University of Technology applied for patents for preparing low-fluorine protein from Antarctic krill in 2010 and 2011, respectively.
Similarly, Antarctic krill hydrolyzed polypeptide also has the problem of high fluorine content, which limits the further processing and utilization of Antarctic krill. Therefore, it is extremely important to develop low-fluorine hydrolyzed polypeptide technology for Antarctic krill
At present, there are few research reports on the removal of fluorine in Antarctic krill hydrolyzed polypeptide products. Ocean University of China adopts nanofiltration technology, electrodialysis technology and calcium chloride sedimentation method to remove fluorine in enzymatic hydrolyzate, and adopts nanofiltration technology and The method of electrodialysis defluorination, the loss

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Mixing: mix the Antarctic krill dry powder with an oil content of 20% and water according to a mass ratio of 1:5, and then stir evenly;

[0017] (2) Enzymatic hydrolysis: carry out thermal enzymatic hydrolysis on the mixed krill meal suspension, select neutral protease for enzymatic hydrolysis, adjust the optimum pH value of the enzyme to 7.0, and the quality of the enzyme is the dry weight of krill meal. 0.5%, the enzymatic hydrolysis temperature is 50 °C, and the enzymatic hydrolysis time is 6 hours;

[0018] (3) Enzyme inactivation: after enzymolysis for a certain period of time, the enzyme is inactivated by boiling and heating, and the time is 5 minutes;

[0019] (4) Centrifugation: the solution after the enzyme inactivation is cooled and then centrifuged, the rotating speed is 3000rpm, the temperature is 10°C, and the centrifugation time is 30 minutes;

[0020] (5) Defluorination: defluorination is performed on the centrifuged supernatant, calcium oxide with 0...

Embodiment 2

[0025] (1) Mixing: mix the Antarctic krill dry powder with an oil content of 10% and water according to a mass ratio of 1:2, and then stir evenly;

[0026] (2) Enzymatic hydrolysis: carry out thermal enzymatic hydrolysis on the mixed krill meal suspension, select alkaline protease for enzymatic hydrolysis, adjust the optimum pH value of the enzyme to 9.0, and the quality of the enzyme is the dry weight of krill meal. 1.0%, the enzymatic hydrolysis temperature is 60°C, and the enzymatic hydrolysis time is 3 hours;

[0027] (3) Enzyme inactivation: after enzymolysis for a certain period of time, the enzyme is inactivated by heating in a boiling water bath, and the time is 10 minutes;

[0028] (4) Centrifugation: the solution after the enzyme inactivation is cooled and then centrifuged, the rotating speed is 5000rpm, the temperature is 20°C, and the centrifugation time is 20 minutes;

[0029] (5) Defluorination: defluorination is performed on the centrifuged supernatant, calcium...

Embodiment 3

[0034] (1) Mixing: mix the degreasing Antarctic krill dry powder with water in a ratio of 1:8 by mass, and then stir evenly;

[0035] (2) Enzymatic hydrolysis: carry out thermal enzymatic hydrolysis on the mixed krill meal suspension, select trypsin for enzymatic hydrolysis, adjust the optimum pH value of the enzyme to 7.5, and the quality of the enzyme is 2.0 of the dry weight of krill meal. %, the enzymatic hydrolysis temperature is 37 °C, and the enzymatic hydrolysis time is 6 hours;

[0036] (3) Enzyme inactivation: after enzymolysis for a certain period of time, the enzyme is inactivated by heating in a boiling water bath, and the time is 20 minutes;

[0037] (4) Centrifugation: the solution after the enzyme inactivation is cooled and then centrifuged, the rotating speed is 6000rpm, the temperature is 30°C, and the centrifugation time is 15 minutes;

[0038] (5) Defluorination: defluorination is performed on the centrifuged supernatant, calcium oxide of 0.8% of the solut...

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Abstract

The invention discloses a method for preparing antarctic krill low-fluorine hydrolysis polypeptide. The method comprises the following steps of: performing enzymolysis on antarctic krill powder used as raw materials, heating and inactivating enzyme, centrifuging, adding calcium oxide at different concentration, defluorinating, uniformly stirring, performing centrifugal separation, adjusting a solution until the solution has a certain pH value, and performing spray drying on supernatant obtained through separation to obtain the antarctic krill low-fluorine hydrolysis polypeptide of which the fluorine content is 10 to 30ppm. The process is easy to operate, the obtained product has low fluorine content, the nutrition constituents are damaged slightly, consumed time is short, and the process is simple and efficient, and is an ideal method for removing fluorine from enzymatic hydrolysate. The method lays the foundation for developing the multi-functional antarctic krill low-fluorine polypeptide.

Description

technical field [0001] The invention relates to a method for preparing small molecular polypeptides from Antarctic krill meal, in particular to a method for preparing low-fluorine hydrolyzed polypeptides. Background technique [0002] Antarctic krill is one of the most abundant biological species on earth. According to statistics, the biomass of Antarctic krill resources is conservatively estimated to be about 650 to 1 billion tons. Antarctic krill is the species with the highest protein content among the organisms discovered by humans so far. The protein content is high and excellent. The protein content of krill meat is 17.65%, and that of dried krill is as high as 65%, which is the second highest of other animal foods. three times. Antarctic krill contains all the amino acids necessary for the human body, especially the lysine content is very rich, accounting for 9.73% of the protein content. Antarctic krill enzymatic hydrolysate is rich in various amino acids, active ...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 余奕珂胥亚夫刘建君苏学锋王元好张庆香谭玉喜袁起新
Owner 辽渔集团有限公司
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