Method for preparing antarctic krill low-fluorine hydrolysis polypeptide

Abstract

The invention discloses a method for preparing antarctic krill low-fluorine hydrolysis polypeptide. The method comprises the following steps of: performing enzymolysis on antarctic krill powder used as raw materials, heating and inactivating enzyme, centrifuging, adding calcium oxide at different concentration, defluorinating, uniformly stirring, performing centrifugal separation, adjusting a solution until the solution has a certain pH value, and performing spray drying on supernatant obtained through separation to obtain the antarctic krill low-fluorine hydrolysis polypeptide of which the fluorine content is 10 to 30ppm. The process is easy to operate, the obtained product has low fluorine content, the nutrition constituents are damaged slightly, consumed time is short, and the process is simple and efficient, and is an ideal method for removing fluorine from enzymatic hydrolysate. The method lays the foundation for developing the multi-functional antarctic krill low-fluorine polypeptide.

Description

technical field [0001] The invention relates to a method for preparing small molecular polypeptides from Antarctic krill meal, in particular to a method for preparing low-fluorine hydrolyzed polypeptides. Background technique [0002] Antarctic krill is one of the most abundant biological species on earth. According to statistics, the biomass of Antarctic krill resources is conservatively estimated to be about 650 to 1 billion tons. Antarctic krill is the species with the highest protein content among the organisms discovered by humans so far. The protein content is high and excellent. The protein content of krill meat is 17.65%, and that of dried krill is as high as 65%, which is the second highest of other animal foods. three times. Antarctic krill contains all the amino acids necessary for the human body, especially the lysine content is very rich, accounting for 9.73% of the protein content. Antarctic krill enzymatic hydrolysate is rich in various amino acids, active ...

AI Technical Summary

Problems solved by technology

[0003] However, there is a problem of high fluorine content in Antarctic krill. Ocean University of China and Dalian University of Technology applied for patents for preparing low-fluorine protein from Antarctic krill in 2010 and 2011, respectively.

Similarly, Antarctic krill hydrolyzed polypeptide also has the problem of high fluorine content, which limits the further processing and utilization of Antarctic krill. Therefore, it is extremely important to develop low-fluorine hydrolyzed polypeptide technology for Antarctic krill

At present, there are few research reports on the removal of fluorine in Antarctic krill hydrolyzed polypeptide products. Ocean University of China adopts nanofiltration technology, electrodialysis technology and calcium chloride sedimentation method to remove fluorine in enzymatic hydrolyzate, and adopts nanofiltration technology and The method of electrodialysis defluorination, the loss of nutrients such as total nitrogen and amino nitrogen is relatively serious, and the loss of total nitrogen is more than 15-20%, and the comprehensive effect is not ideal

Calcium chloride sedimentation method is used for defluorination, the amount of calcium chloride added is 1.38% of the solution mass, and then the pH value of the solution must be adjusted, and other acid-base components must be repeatedly added to adjust the pH value of the solution. The fluorine content of the enzymatically hydrolyzed polypeptide can reach 20-30mg / kg, and the total nitrogen loss is about 8%. However, with this method, the amount of calcium chloride added is relatively large, which is likely to cause excess calcium residues and remove excess calcium. It will cause the loss of active ingredients and nutrients, waste energy, and the process is complicated. After mixing calcium chloride, it is necessary to adjust the pH value