Process method for extracting superoxide dismutase from root, stem and leaf of burdock

A process method, superoxide technology, applied in the direction of oxidoreductase, peptide preparation method, chemical instrument and method, etc., can solve the problems of loss of SOD, abandonment, environmental pollution, etc.

Active Publication Date: 2012-07-18
龚森淼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the burdock stems and leaves of the current burdock production bases in my country are all abandoned as a burden, causing environmental pollution
Little do they know that the most valuable resources are wasted, and at the same time, the environment is greatly polluted
There are also problems in the consumption of burdock rhizomes, which will greatly lose SOD, various proteases, various vitamins and various active ingredients through high-temperature processing

Method used

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  • Process method for extracting superoxide dismutase from root, stem and leaf of burdock

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] The cleaned burdock stems and leaves are mashed with a tissue masher, and 0.001%-0.1% sodium erythorbate is added in the mashing process to prevent SOD and other active components from being oxidized. Then homogenize with a homogenizer, centrifuge with a refrigerated centrifuge at 5,000rpm-10,000rpm for 5-15 minutes, and pass ultrafiltration twice on the obtained supernatant. For the first time, use an ultrafiltration membrane with a cut-off of more than 60,000 Daltons, usually a hollow fiber ultrafiltration membrane, with a working pressure of 0.15MPa; the second ultrafiltration is to use an ultrafiltration membrane with a cut-off of more than 10,000 Daltons Ultrafiltration, usually with hollow fiber ultrafiltration membrane, the working pressure is 0.15Mpa. The 60,000 to 10,000 Dalton concentrate obtained by two ultrafiltrations is a solution containing SOD substances. The first ultrafiltration membrane material: polysulfone, membrane area: 0.12M 2 : Diameter: 10cm,...

Embodiment 2

[0009] The burdock rhizomes that have been washed and undergone sugar conversion are mashed with a tissue masher, and 0.001%-0.1% sodium erythorbate is added in the mash process to prevent SOD and other active components from being oxidized. Then homogenize with a homogenizer, centrifuge with a refrigerated centrifuge at 5,000rpm-10,000rpm for 5-15 minutes, and pass ultrafiltration twice on the obtained supernatant. The first ultrafiltration is performed with an ultrafiltration membrane with a cut-off of 60,000 Daltons, usually a hollow fiber ultrafiltration membrane, and the working pressure is 0.15MPa; the second ultrafiltration is performed with an ultrafiltration membrane with a cut-off of 10,000 Daltons , usually with hollow fiber ultrafiltration membrane, the working pressure is 0.15Mpa. The 60,000 to 10,000 Dalton concentrate obtained by two ultrafiltrations is a solution containing SOD substances. The first ultrafiltration membrane material: polysulfone, membrane area...

Embodiment 3

[0011] The cleaned burdock stems and leaves are mashed with a tissue masher, and 0.001%-0.1% sodium erythorbate is added in the mashing process to prevent SOD and other active components from being oxidized. Then homogenize with a homogenizer, centrifuge with a refrigerated centrifuge at 5,000rpm-10,000rpm for 5-15 minutes, and pass ultrafiltration twice on the obtained supernatant. For the first time, ultrafiltration with a flat ultrafiltration membrane with a cutoff of more than 60,000 Daltons was used, and the working pressure was 0.17 MPa; the second ultrafiltration was performed with a flat ultrafiltration membrane with a cutoff of more than 10,000 Daltons, and the working pressure was 0.17Mpa. The 60,000 to 10,000 Dalton concentrate obtained by two ultrafiltrations is a solution containing SOD substances. The first ultrafiltration membrane material: polysulfone, membrane area: 0.15M 2 : Length: 30cm; Width: 15cm; Height: 5cm, 0.17MPa, ultrafiltration for 45 minutes. S...

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Abstract

The invention relates to a process method for extracting superoxide dismutase from a root, a stem and a leaf of a burdock, which comprises the following steps of: cleaning the root, the stem and the leaf of the burdock, organizing, smashing, homogenating and carrying out frozen separation; successively ultra-filtering separated supernate for two times; intercepting above sixty thousands of daltons of matters in the primary ultrafiltration and intercepting above ten thousands of daltons of matters in the secondary ultrafiltration; and intercepting solution passing through the primary ultrafiltration into ten thousands-sixty thousands of solution containing SOD (Superoxide Dismutase) by the secondary ultrafiltration, and after the solution containing the SOD intercepted in the secondary ultrafiltration is dried by PEG (Polyethylene Glycol), drying in a freeze drier to obtain a solid SOD matter.

Description

technical field [0001] The invention relates to a process for extracting superoxide dismutase SOD from roots, stems, fruits and leaves of plants, more specifically a process for extracting superoxide dismutase from roots, stems and leaves of burdock. Background technique [0002] Superoxide dismutase, English is Superoxide Dismutase, abbreviated as SOD, SOD exists in the cells of plants, animals and microorganisms, in the human body, SOD can remove excess superoxide anion free radicals and hydroxyl free radicals in the human body, and has anti-oxidative properties , anti-aging, enhancing immunity and various effects, more and more people pay attention to it. Due to the large-scale mad cow disease and poultry diseases that have occurred in the world in recent years, it is very easy to infect humans and cause huge disasters. The World Health Organization (WHO) appeals not to extract SOD from animal plasma and use it for humans. [0003] Plant SOD is relatively safe to use, bu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02A23K1/14C05F5/00C07K14/415C07K1/34A23L1/214A23L1/29A23L19/10A23L33/00
CPCY02A40/20
Inventor 龚家骅梅汝鸿梅宁
Owner 龚森淼
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