Method for preparing melanin and precursors thereof by utilizing complex enzyme

Abstract

The invention provides a method for preparing melanin and precursors thereof by utilizing a complex enzyme. The method comprises the following steps: utilizing the complex enzyme containing tyrosinase, which is extracted from bacillus fermentation liquid producing the melanin, taking tyrosine and / or a derivative thereof as a substrate, and generating the melanin and the precursors thereof by enzymatic reaction. The complex enzyme used by the method is stable in source, high in enzyme activity and high in production efficiency; the production of a series of the melanin precursors through the enzymatic reaction method is simple, convenient and easy to operate, the enzyme can be further used repeatedly through a carrier immobilization method, the cost is further reduced, the production efficiency is improved, and the prepared melanin precursors can be used as effective hair dyes and antioxidants.

Description

technical field [0001] The invention relates to the fields of fermentation and enzyme immobilization, in particular to a method for preparing melanin and its precursors by using complex enzymes. Background technique [0002] Melanin is an important class of natural pigment widely present in animals, plants and microorganisms. It has a complex structure and is generally a heterogeneous polyphenol-like polymer. Melanin in animal tissues does not exist in the form of a single melanin, but a mixture of two types of eumelanin and amelanin, so it shows a variety of colors of skin and coat. Melanin in the living body is formed by oxidizing tyrosine and cysteine ​​into eumelanin and demelanin through a series of enzymatic reactions through complex enzymes such as tyrosinase and tautomerase Natural melanin ( figure 1 and 2 ). The precursors of melanin include dopachrome, 5,6-dihydroxyindole, 5,6-dihydroxyindole-2-carboxylic acid, etc., which have anti-oxidation, anti-radiation an...

AI Technical Summary

Problems solved by technology

At present, there are two ways to produce melanin. One is to pickling the seed coats of grains such as black beans, black sesame, sorghum, etc., and the output is small and the cost is high; the other is to separate fermented black yeast from yeast cells. Difficult strain separation, complex equipment, large investment

[0004] CN1420144A extracts tyrosine oxidase from plants, uses tyrosine monohydric phenol derivatives as substrates, and controls enzymatic synthesis reaction conditions to synthesize melanin. The enzyme used is relatively simple, which affects the enzymatic efficiency and cannot be used repeatedly. In addition, the cost of extracting tyrosinase from plants is relatively high

[0005] The use of microorganisms to produce melanin, especially the use of complex enzymes extracted from the fermentation broth of melanin-producing bacillus to prepare natural melanin, thereby preparing hair dyes has not yet been reported

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