Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting peganum harmala alkaloid

A technology of camel pod and alkaloids, which is applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problems of high energy consumption, large amount of toxic reagents, serious pollution, etc., and achieves low energy consumption, pollution reduction, and waste liquid reduction. produced effect

Inactive Publication Date: 2012-07-25
苏州宝泽堂医药科技有限公司
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] As mentioned above, existing methods exist: high energy consumption, serious pollution, and large amount of toxic reagents.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take Camelia seeds and crush them, take 5kg, add 100kg of 0.05% hydrochloric acid water to percolate for 20 hours, filter the percolation liquid, add 2LNK-9 macroporous resin column to absorb impurities, collect the column passing liquid, add hollow fiber with a molecular weight cut off of 3000 Plain membrane ultrafiltration, normal temperature, inlet pressure 0.6-0.8MPa, add appropriate amount of water for dialysis, collect the permeate, then add hollow cellulose membrane with a molecular weight cut-off of 200 to concentrate to obtain a concentrated solution, add ammonia water to the concentrated solution to adjust pH7.5, and then add Adsorbed on a 3L SPD100 macroporous resin column, washed with water until neutral, and then eluted with 21L of 60% ethanol. The collected eluate was concentrated under reduced pressure, and the concentrated solution was vacuum-dried to obtain 670g of camel alkaloid, which was detected by titration with a content of 98.2%.

Embodiment 2

[0021] Take the whole camel grass and crush it, take 5kg, add 75kg of 0.5% hydrochloric acid water to percolate for 15 hours, filter the percolation liquid, add 2LHZ806 macroporous resin column to absorb impurities, and add hollow cellulose membrane with molecular weight cut-off 6000 to the liquid collected through the column Ultrafiltration, normal temperature, inlet pressure 0.5-0.6MPa, add appropriate amount of water for dialysis, collect the permeate, then add hollow cellulose membrane with a molecular weight cut-off of 100 to concentrate to obtain a concentrated solution, add ammonia water to the concentrated solution to adjust pH8, then add 3L AB-8 Adsorbed on a macroporous resin column, washed with water until neutral, and then eluted with 12L of 80% ethanol, collected eluent was concentrated under reduced pressure, and the concentrated solution was vacuum-dried to obtain 135g of camel alkaloid, which was detected by titration with a content of 95.2%.

Embodiment 3

[0023] Take the camel root and crush it, take 5kg, add 100kg of 0.05% sulfuric acid water to percolate for 20 hours, filter the percolation liquid, add 2L S-8 macroporous resin column to absorb impurities, collect the column passing liquid, add a hollow Cellulose membrane ultrafiltration, normal temperature, inlet pressure 0.6-0.8MPa, add appropriate amount of water for dialysis, collect the permeate and then add hollow cellulose membrane with a molecular weight cut-off of 200 to concentrate to obtain a concentrate, add ammonia water to the concentrate to adjust pH7.5, and then Add 3L of HZ818 macroporous resin column for adsorption, wash with water until neutral, then elute with 18L of 70% ethanol, collect the eluate and concentrate under reduced pressure, and vacuum-dry the concentrated solution to obtain 162g of camel alkaloid, which is 97.2% by titration detection.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting peganum harmala alkaloid, which comprises the steps that 1) peganum harmala raw materials are smashed and mixed with acid water to be percolated to obtain diacolation liquid; 2) the diacolation liquid is filled into a macroporous resin column to be adsorbed, liquid passing through the macroporous resin column is filtered by an ultrafiltration membrane, and the liquid passing through the ultrafiltration membrane is concentrated by a nanofiltration membrane to obtain concentration liquid; and 3) a pH value of the concentration liquid is adjusted to 7.5 to 8 by adding ammonia, then the mixed solution is adsorbed by the macroporous resin column, the pH value is adjusted by water washing to neutral, then 4 to 7BV 60 to 80 percent of ethanol is used for elution, the elution liquid is collected, decompressed and concentrated, and the concentration liquid is vacuum dried to obtain a product. By adopting the method to produce the peganum harmala alkaloid, the technique is simple to operate, energy consumption is low, and pollution is small. By adopting the technique, the industrialized production is easy to realize.

Description

Technical field: [0001] The invention relates to a method for extracting camel bald alkaloids, in particular to a method for extracting and purifying camel bald alkaloids by adopting macroporous resin adsorption and membrane separation. Background technique: [0002] Peganum harmala L., also known as Artemisia camel, bitter bitter vegetable, stinky grass, and stinky peony, is the whole herb of the perennial herb of the genus Peganum of the family ZygopHyllaceae. It is flat in nature, bitter in taste, pungent, and poisonous. It has the functions of strengthening tendons, helping yang and warming yin, eliminating viscous body fluids, and dissipating cold and damp air. Indications for weakness of tendons, arthralgia, cough with profuse phlegm, hemiplegia, forgetfulness, dizziness, headache, and irregular menstruation. [0003] The whole camel grass contains alkaloids, steroids, flavonoids, anthraquinones, amino acids and other components. Among them, there are the most types ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P9/00A61P11/00A61P31/04A61P31/10A61P31/12A61P29/00A61P33/00A61P35/00A61P37/02A61K125/00A61K131/00
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com